Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
In Brazil, human and canine visceral leishmaniasis (CVL) caused byLeishmania infantum has undergone urbanisation since 1980, constituting a public health problem, and serological tests are tools of choice for identifying infected dogs. Until recently, the Brazilian zoonoses control program recommend...
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ftdoajarticles:oai:doaj.org/article:1f0872519c404eada9ae8585090846cc 2023-05-15T15:07:13+02:00 Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection Mauro Maciel de Arruda Fabiano Borges Figueiredo Andreza Pain Marcelino José Ronaldo Barbosa Guilherme Loureiro Werneck Elza Ferreira Noronha Gustavo Adolfo Sierra Romero 2016-01-01T00:00:00Z https://doi.org/10.1590/0074-02760150364 https://doaj.org/article/1f0872519c404eada9ae8585090846cc EN eng Instituto Oswaldo Cruz, Ministério da Saúde http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016005003102&lng=en&tlng=en https://doaj.org/toc/1678-8060 1678-8060 doi:10.1590/0074-02760150364 https://doaj.org/article/1f0872519c404eada9ae8585090846cc Memórias do Instituto Oswaldo Cruz., Iss 0 (2016) dogs sensitivity specificity visceral leishmaniasis Leishmania infantum serology Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 article 2016 ftdoajarticles https://doi.org/10.1590/0074-02760150364 2023-01-08T01:32:12Z In Brazil, human and canine visceral leishmaniasis (CVL) caused byLeishmania infantum has undergone urbanisation since 1980, constituting a public health problem, and serological tests are tools of choice for identifying infected dogs. Until recently, the Brazilian zoonoses control program recommended enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays (IFA) as the screening and confirmatory methods, respectively, for the detection of canine infection. The purpose of this study was to estimate the accuracy of ELISA and IFA in parallel or serial combinations. The reference standard comprised the results of direct visualisation of parasites in histological sections, immunohistochemical test, or isolation of the parasite in culture. Samples from 98 cases and 1,327 noncases were included. Individually, both tests presented sensitivity of 91.8% and 90.8%, and specificity of 83.4 and 53.4%, for the ELISA and IFA, respectively. When tests were used in parallel combination, sensitivity attained 99.2%, while specificity dropped to 44.8%. When used in serial combination (ELISA followed by IFA), decreased sensitivity (83.3%) and increased specificity (92.5%) were observed. Serial testing approach improved specificity with moderate loss in sensitivity. This strategy could partially fulfill the needs of public health and dog owners for a more accurate diagnosis of CVL. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Memórias do Instituto Oswaldo Cruz 111 3 168 173 |
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dogs sensitivity specificity visceral leishmaniasis Leishmania infantum serology Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
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dogs sensitivity specificity visceral leishmaniasis Leishmania infantum serology Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 Mauro Maciel de Arruda Fabiano Borges Figueiredo Andreza Pain Marcelino José Ronaldo Barbosa Guilherme Loureiro Werneck Elza Ferreira Noronha Gustavo Adolfo Sierra Romero Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection |
topic_facet |
dogs sensitivity specificity visceral leishmaniasis Leishmania infantum serology Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
description |
In Brazil, human and canine visceral leishmaniasis (CVL) caused byLeishmania infantum has undergone urbanisation since 1980, constituting a public health problem, and serological tests are tools of choice for identifying infected dogs. Until recently, the Brazilian zoonoses control program recommended enzyme-linked immunosorbent assays (ELISA) and indirect immunofluorescence assays (IFA) as the screening and confirmatory methods, respectively, for the detection of canine infection. The purpose of this study was to estimate the accuracy of ELISA and IFA in parallel or serial combinations. The reference standard comprised the results of direct visualisation of parasites in histological sections, immunohistochemical test, or isolation of the parasite in culture. Samples from 98 cases and 1,327 noncases were included. Individually, both tests presented sensitivity of 91.8% and 90.8%, and specificity of 83.4 and 53.4%, for the ELISA and IFA, respectively. When tests were used in parallel combination, sensitivity attained 99.2%, while specificity dropped to 44.8%. When used in serial combination (ELISA followed by IFA), decreased sensitivity (83.3%) and increased specificity (92.5%) were observed. Serial testing approach improved specificity with moderate loss in sensitivity. This strategy could partially fulfill the needs of public health and dog owners for a more accurate diagnosis of CVL. |
format |
Article in Journal/Newspaper |
author |
Mauro Maciel de Arruda Fabiano Borges Figueiredo Andreza Pain Marcelino José Ronaldo Barbosa Guilherme Loureiro Werneck Elza Ferreira Noronha Gustavo Adolfo Sierra Romero |
author_facet |
Mauro Maciel de Arruda Fabiano Borges Figueiredo Andreza Pain Marcelino José Ronaldo Barbosa Guilherme Loureiro Werneck Elza Ferreira Noronha Gustavo Adolfo Sierra Romero |
author_sort |
Mauro Maciel de Arruda |
title |
Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection |
title_short |
Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection |
title_full |
Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection |
title_fullStr |
Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection |
title_full_unstemmed |
Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection |
title_sort |
sensitivity and specificity of parallel or serial serological testing for detection of canine leishmania infection |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2016 |
url |
https://doi.org/10.1590/0074-02760150364 https://doaj.org/article/1f0872519c404eada9ae8585090846cc |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Memórias do Instituto Oswaldo Cruz., Iss 0 (2016) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016005003102&lng=en&tlng=en https://doaj.org/toc/1678-8060 1678-8060 doi:10.1590/0074-02760150364 https://doaj.org/article/1f0872519c404eada9ae8585090846cc |
op_doi |
https://doi.org/10.1590/0074-02760150364 |
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Memórias do Instituto Oswaldo Cruz |
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111 |
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3 |
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