Metabolic profiling of an Echinostoma caproni infection in the mouse for biomarker discovery.
BACKGROUND: Metabolic profiling holds promise with regard to deepening our understanding of infection biology and disease states. The objectives of our study were to assess the global metabolic responses to an Echinostoma caproni infection in the mouse, and to compare the biomarkers extracted from d...
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ftdoajarticles:oai:doaj.org/article:1ef0ff96e9e140eaa761bb115c3aa10d 2023-05-15T15:15:24+02:00 Metabolic profiling of an Echinostoma caproni infection in the mouse for biomarker discovery. Jasmina Saric Jia V Li Yulan Wang Jennifer Keiser Jake G Bundy Elaine Holmes Jürg Utzinger 2008-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000254 https://doaj.org/article/1ef0ff96e9e140eaa761bb115c3aa10d EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2432044?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000254 https://doaj.org/article/1ef0ff96e9e140eaa761bb115c3aa10d PLoS Neglected Tropical Diseases, Vol 2, Iss 7, p e254 (2008) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2008 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000254 2022-12-31T03:30:34Z BACKGROUND: Metabolic profiling holds promise with regard to deepening our understanding of infection biology and disease states. The objectives of our study were to assess the global metabolic responses to an Echinostoma caproni infection in the mouse, and to compare the biomarkers extracted from different biofluids (plasma, stool, and urine) in terms of characterizing acute and chronic stages of this intestinal fluke infection. METHODOLOGY/PRINCIPAL FINDINGS: Twelve female NMRI mice were infected with 30 E. caproni metacercariae each. Plasma, stool, and urine samples were collected at 7 time points up to day 33 post-infection. Samples were also obtained from non-infected control mice at the same time points and measured using (1)H nuclear magnetic resonance (NMR) spectroscopy. Spectral data were subjected to multivariate statistical analyses. In plasma and urine, an altered metabolic profile was already evident 1 day post-infection, characterized by reduced levels of plasma choline, acetate, formate, and lactate, coupled with increased levels of plasma glucose, and relatively lower concentrations of urinary creatine. The main changes in the urine metabolic profile started at day 8 post-infection, characterized by increased relative concentrations of trimethylamine and phenylacetylglycine and lower levels of 2-ketoisocaproate and showed differentiation over the course of the infection. CONCLUSION/SIGNIFICANCE: The current investigation is part of a broader NMR-based metabonomics profiling strategy and confirms the utility of this approach for biomarker discovery. In the case of E. caproni, a diagnosis based on all three biofluids would deliver the most comprehensive fingerprint of an infection. For practical purposes, however, future diagnosis might aim at a single biofluid, in which case urine would be chosen for further investigation, based on quantity of biomarkers, ease of sampling, and the degree of differentiation from the non-infected control group. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 2 7 e254 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jasmina Saric Jia V Li Yulan Wang Jennifer Keiser Jake G Bundy Elaine Holmes Jürg Utzinger Metabolic profiling of an Echinostoma caproni infection in the mouse for biomarker discovery. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Metabolic profiling holds promise with regard to deepening our understanding of infection biology and disease states. The objectives of our study were to assess the global metabolic responses to an Echinostoma caproni infection in the mouse, and to compare the biomarkers extracted from different biofluids (plasma, stool, and urine) in terms of characterizing acute and chronic stages of this intestinal fluke infection. METHODOLOGY/PRINCIPAL FINDINGS: Twelve female NMRI mice were infected with 30 E. caproni metacercariae each. Plasma, stool, and urine samples were collected at 7 time points up to day 33 post-infection. Samples were also obtained from non-infected control mice at the same time points and measured using (1)H nuclear magnetic resonance (NMR) spectroscopy. Spectral data were subjected to multivariate statistical analyses. In plasma and urine, an altered metabolic profile was already evident 1 day post-infection, characterized by reduced levels of plasma choline, acetate, formate, and lactate, coupled with increased levels of plasma glucose, and relatively lower concentrations of urinary creatine. The main changes in the urine metabolic profile started at day 8 post-infection, characterized by increased relative concentrations of trimethylamine and phenylacetylglycine and lower levels of 2-ketoisocaproate and showed differentiation over the course of the infection. CONCLUSION/SIGNIFICANCE: The current investigation is part of a broader NMR-based metabonomics profiling strategy and confirms the utility of this approach for biomarker discovery. In the case of E. caproni, a diagnosis based on all three biofluids would deliver the most comprehensive fingerprint of an infection. For practical purposes, however, future diagnosis might aim at a single biofluid, in which case urine would be chosen for further investigation, based on quantity of biomarkers, ease of sampling, and the degree of differentiation from the non-infected control group. |
format |
Article in Journal/Newspaper |
author |
Jasmina Saric Jia V Li Yulan Wang Jennifer Keiser Jake G Bundy Elaine Holmes Jürg Utzinger |
author_facet |
Jasmina Saric Jia V Li Yulan Wang Jennifer Keiser Jake G Bundy Elaine Holmes Jürg Utzinger |
author_sort |
Jasmina Saric |
title |
Metabolic profiling of an Echinostoma caproni infection in the mouse for biomarker discovery. |
title_short |
Metabolic profiling of an Echinostoma caproni infection in the mouse for biomarker discovery. |
title_full |
Metabolic profiling of an Echinostoma caproni infection in the mouse for biomarker discovery. |
title_fullStr |
Metabolic profiling of an Echinostoma caproni infection in the mouse for biomarker discovery. |
title_full_unstemmed |
Metabolic profiling of an Echinostoma caproni infection in the mouse for biomarker discovery. |
title_sort |
metabolic profiling of an echinostoma caproni infection in the mouse for biomarker discovery. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2008 |
url |
https://doi.org/10.1371/journal.pntd.0000254 https://doaj.org/article/1ef0ff96e9e140eaa761bb115c3aa10d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 2, Iss 7, p e254 (2008) |
op_relation |
http://europepmc.org/articles/PMC2432044?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0000254 https://doaj.org/article/1ef0ff96e9e140eaa761bb115c3aa10d |
op_doi |
https://doi.org/10.1371/journal.pntd.0000254 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
2 |
container_issue |
7 |
container_start_page |
e254 |
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1766345764657692672 |