Calmodulin regulates the calcium homeostasis in mantle of Crassostrea gigas under ocean acidification
The biosynthesis of shell is a complicated calcification process in the marine bivalve, which can be severely impacted by ocean acidification (OA). Calmodulin (CaM) is a pivotal calcium regulator and thought to be crucial for calcification. In the present study, a CaM (designated CgCaM) with calcium...
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Online Access: | https://doi.org/10.3389/fmars.2022.1050022 https://doaj.org/article/1ee639e94dab4f2cb98e6fa760fa32af |
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ftdoajarticles:oai:doaj.org/article:1ee639e94dab4f2cb98e6fa760fa32af 2023-05-15T15:57:42+02:00 Calmodulin regulates the calcium homeostasis in mantle of Crassostrea gigas under ocean acidification Xiaoyu Xin Chang Liu Zhaoqun Liu Yukun Zhang Yuqian Gao Ting Zhu Lingling Wang Linsheng Song 2022-11-01T00:00:00Z https://doi.org/10.3389/fmars.2022.1050022 https://doaj.org/article/1ee639e94dab4f2cb98e6fa760fa32af EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2022.1050022/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.1050022 https://doaj.org/article/1ee639e94dab4f2cb98e6fa760fa32af Frontiers in Marine Science, Vol 9 (2022) ocean acidification calmodulin calcium homeostasis mantle Crassostrea gigas Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2022 ftdoajarticles https://doi.org/10.3389/fmars.2022.1050022 2022-12-30T23:21:30Z The biosynthesis of shell is a complicated calcification process in the marine bivalve, which can be severely impacted by ocean acidification (OA). Calmodulin (CaM) is a pivotal calcium regulator and thought to be crucial for calcification. In the present study, a CaM (designated CgCaM) with calcium-binding activity was identified from the Pacific oyster Crassostrea gigas with the objective to understand its possible role in the regulation of calcium homeostasis under acidification treatment. The open reading frame (ORF) of CgCaM was of 474 bp encoding a 17.5 kDa protein with four continuous EF-hand domains. CgCaM shared high similarity with CaMs from other invertebrates and vertebrates. The mRNA transcript of CgCaM was constitutively expressed in all detected tissues with the higher expression level in mantle, especially highest in the middle fold of the three folds of mantle. CgCaM was found to be mainly distributed in the mantle epithelium. When the oysters were exposed to acidified seawater, the expression level of CgCaM in the middle fold of mantle and the content of Ca2+ in this fold both decreased significantly. These results collectively suggested that CgCaM was involved in the regulation of calcium homeostasis in the middle fold of mantle under acidification treatment. Article in Journal/Newspaper Crassostrea gigas Ocean acidification Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Marine Science 9 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
ocean acidification calmodulin calcium homeostasis mantle Crassostrea gigas Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
ocean acidification calmodulin calcium homeostasis mantle Crassostrea gigas Science Q General. Including nature conservation geographical distribution QH1-199.5 Xiaoyu Xin Chang Liu Zhaoqun Liu Yukun Zhang Yuqian Gao Ting Zhu Lingling Wang Linsheng Song Calmodulin regulates the calcium homeostasis in mantle of Crassostrea gigas under ocean acidification |
topic_facet |
ocean acidification calmodulin calcium homeostasis mantle Crassostrea gigas Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
The biosynthesis of shell is a complicated calcification process in the marine bivalve, which can be severely impacted by ocean acidification (OA). Calmodulin (CaM) is a pivotal calcium regulator and thought to be crucial for calcification. In the present study, a CaM (designated CgCaM) with calcium-binding activity was identified from the Pacific oyster Crassostrea gigas with the objective to understand its possible role in the regulation of calcium homeostasis under acidification treatment. The open reading frame (ORF) of CgCaM was of 474 bp encoding a 17.5 kDa protein with four continuous EF-hand domains. CgCaM shared high similarity with CaMs from other invertebrates and vertebrates. The mRNA transcript of CgCaM was constitutively expressed in all detected tissues with the higher expression level in mantle, especially highest in the middle fold of the three folds of mantle. CgCaM was found to be mainly distributed in the mantle epithelium. When the oysters were exposed to acidified seawater, the expression level of CgCaM in the middle fold of mantle and the content of Ca2+ in this fold both decreased significantly. These results collectively suggested that CgCaM was involved in the regulation of calcium homeostasis in the middle fold of mantle under acidification treatment. |
format |
Article in Journal/Newspaper |
author |
Xiaoyu Xin Chang Liu Zhaoqun Liu Yukun Zhang Yuqian Gao Ting Zhu Lingling Wang Linsheng Song |
author_facet |
Xiaoyu Xin Chang Liu Zhaoqun Liu Yukun Zhang Yuqian Gao Ting Zhu Lingling Wang Linsheng Song |
author_sort |
Xiaoyu Xin |
title |
Calmodulin regulates the calcium homeostasis in mantle of Crassostrea gigas under ocean acidification |
title_short |
Calmodulin regulates the calcium homeostasis in mantle of Crassostrea gigas under ocean acidification |
title_full |
Calmodulin regulates the calcium homeostasis in mantle of Crassostrea gigas under ocean acidification |
title_fullStr |
Calmodulin regulates the calcium homeostasis in mantle of Crassostrea gigas under ocean acidification |
title_full_unstemmed |
Calmodulin regulates the calcium homeostasis in mantle of Crassostrea gigas under ocean acidification |
title_sort |
calmodulin regulates the calcium homeostasis in mantle of crassostrea gigas under ocean acidification |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmars.2022.1050022 https://doaj.org/article/1ee639e94dab4f2cb98e6fa760fa32af |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Ocean acidification Pacific oyster |
genre_facet |
Crassostrea gigas Ocean acidification Pacific oyster |
op_source |
Frontiers in Marine Science, Vol 9 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2022.1050022/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.1050022 https://doaj.org/article/1ee639e94dab4f2cb98e6fa760fa32af |
op_doi |
https://doi.org/10.3389/fmars.2022.1050022 |
container_title |
Frontiers in Marine Science |
container_volume |
9 |
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1766393382266994688 |