Molecular Cloning, Expression Profiles of Insulin-Like Growth Factor-Binding Protein-1 (igfbp-1) and igfbp-2 and their regulation effects on growth of Yellowtail Kingfish (Seriola aureovittata)

• Main Authors: Wenjing ZHANG, Yongjiang XU, Aijun CUI, Bin WANG, Yan JIANG, Kaijie WANG, Heting ZHOU
• Format: Article in Journal/Newspaper
• Language: English; Chinese
• Published: Science Press, PR China 2023
• Subjects: cdna cloning; seriola aureovittata; igfbp-1; igfbp-2; tissue expression; growth regulation; Aquaculture. Fisheries. Angling; SH1-691; Atlantic salmon
• Online Access: https://doi.org/10.19663/j.issn2095-9869.20221213001; https://doaj.org/article/1ec1d6b8835d4784990c9cb222a7a81f

Insulin-like growth factor binding proteins (IGFBPs) play crucial roles in regulating biological activities of insulin-like growth factors (IGFs) and growth performance in vertebrates. IGFBP is a six-member protein family (IGFBP1–6) with a high affinity for IGF. It affects the distribution, stability, and biological activity of IGF by regulating the interaction between IGF ligands and receptors. Recently, IGFBP-1 was identified as a regulator of growth, reproduction, and development in bony fish, such as juvenile Atlantic salmon, where IGFBP-1 interacts with cortisol to regulate growth. IGFBP-1 regulates cell metabolism and growth through interaction with insulin in primary hepatocytes incubated in vitro in orange-spotted grouper (Epinephelus coioides). IGFBP-2 has a wide range of tissue expression characteristics in bony fish, and IGF regulation may occur through autocrine or paracrine pathways. For example, long-term fasting induced increased igfbp-2 mRNA expression in the liver of zebrafish. In addition, IGFBP-2 can inhibit the activity of IGF ligand through its high affinity with IGF to play an inhibitory and regulatory role in zebrafish growth. igfbp-2 mRNA expression was significantly up-regulated in Carassius auratus (goldfish) liver after fasting, and quickly recovered to normal levels after feeding. This indicated that igfbp-2 mRNA expression may be related to the anabolism and catabolism of goldfish, and is regulated by metabolic factors.Yellowtail kingfish (Seriola aureovittata) of the Carangidae family has high economic value and nutrition value. It is a warm and temperate oceanic fish with long distance migration characteristics that is distributed in the middle and upper oceans globally. This species is large with a fast growth rate, and is a promising candidate for aquaculture in land-based industrial circulating water system, deep-water cages, net pen systems, and aquaculture craft, etc. Our laboratory studied the regulatory mechanism of the rapid growth of yellowtail kingfish and cloned gh, ...