In Vitro Antithrombotic Properties of Salmon (Salmo salar) Phospholipids in a Novel Food-Grade Extract
Marine and salmon polar lipids (PLs) extracted by conventional extractions with non-food-grade solvents (CE-salmon-PLs) possess antithrombotic bioactivities against platelet-activating factor (PAF) and thrombin. Similar effects of food-grade-extracted (FGE) marine PLs have not yet been reported. In...
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ftdoajarticles:oai:doaj.org/article:1e99ce9e07e140daa2297b3ad24b333e 2023-05-15T18:09:49+02:00 In Vitro Antithrombotic Properties of Salmon (Salmo salar) Phospholipids in a Novel Food-Grade Extract Alexandros Tsoupras Ronan Lordan Katie Shiels Sushanta Kumar Saha Constantina Nasopoulou Ioannis Zabetakis 2019-01-01T00:00:00Z https://doi.org/10.3390/md17010062 https://doaj.org/article/1e99ce9e07e140daa2297b3ad24b333e EN eng MDPI AG http://www.mdpi.com/1660-3397/17/1/62 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md17010062 https://doaj.org/article/1e99ce9e07e140daa2297b3ad24b333e Marine Drugs, Vol 17, Iss 1, p 62 (2019) salmon polar lipids platelet aggregation platelet-activating factor (PAF) thrombin phosphatidylcholine phosphatidylethanolamine LC-MS EPA DHA PUFA phospholipids Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.3390/md17010062 2022-12-30T23:39:53Z Marine and salmon polar lipids (PLs) extracted by conventional extractions with non-food-grade solvents (CE-salmon-PLs) possess antithrombotic bioactivities against platelet-activating factor (PAF) and thrombin. Similar effects of food-grade-extracted (FGE) marine PLs have not yet been reported. In this study, food-grade solvents were used to extract PLs from Irish organic farmed salmon (Salmo salar) fillets (FGE-salmon-PLs), while their antithrombotic bioactivities were assessed in human platelets induced by platelet aggregation agonists (PAF/thrombin). FGE-salmon-PLs were further separated by thin layer chromatography (TLC) into lipid subclasses, and the antithrombotic bioactivities of each subclass were also assessed. LC-MS was utilized to elucidate the structure-activity relationships. FGE-salmon-PLs strongly inhibited PAF-induced platelet aggregation, while their relevant anti-thrombin effects were at least three times more potent than the previously reported activities of CE-salmon-PLs. TLC-derived lipid fractions corresponding to phosphatidylcholines (PC) and phosphatidylethanolamines (PE) were the most bioactive lipid subclasses obtained, especially against thrombin. Their LC-MS analysis elucidated that they are diacyl- or alkyl-acyl- PC and PE moieties baring ω3 polyunsaturated fatty acids (PUFA) at their sn-2 position, such as eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA). Our results concerning the potent antithrombotic effects of FGE-salmon-PLs against both PAF and thrombin pathways strongly suggest that such food-grade extracts are putative candidates for the development of novel cardioprotective supplements and nutraceuticals. Article in Journal/Newspaper Salmo salar Directory of Open Access Journals: DOAJ Articles Marine Drugs 17 1 62 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
salmon polar lipids platelet aggregation platelet-activating factor (PAF) thrombin phosphatidylcholine phosphatidylethanolamine LC-MS EPA DHA PUFA phospholipids Biology (General) QH301-705.5 |
spellingShingle |
salmon polar lipids platelet aggregation platelet-activating factor (PAF) thrombin phosphatidylcholine phosphatidylethanolamine LC-MS EPA DHA PUFA phospholipids Biology (General) QH301-705.5 Alexandros Tsoupras Ronan Lordan Katie Shiels Sushanta Kumar Saha Constantina Nasopoulou Ioannis Zabetakis In Vitro Antithrombotic Properties of Salmon (Salmo salar) Phospholipids in a Novel Food-Grade Extract |
topic_facet |
salmon polar lipids platelet aggregation platelet-activating factor (PAF) thrombin phosphatidylcholine phosphatidylethanolamine LC-MS EPA DHA PUFA phospholipids Biology (General) QH301-705.5 |
description |
Marine and salmon polar lipids (PLs) extracted by conventional extractions with non-food-grade solvents (CE-salmon-PLs) possess antithrombotic bioactivities against platelet-activating factor (PAF) and thrombin. Similar effects of food-grade-extracted (FGE) marine PLs have not yet been reported. In this study, food-grade solvents were used to extract PLs from Irish organic farmed salmon (Salmo salar) fillets (FGE-salmon-PLs), while their antithrombotic bioactivities were assessed in human platelets induced by platelet aggregation agonists (PAF/thrombin). FGE-salmon-PLs were further separated by thin layer chromatography (TLC) into lipid subclasses, and the antithrombotic bioactivities of each subclass were also assessed. LC-MS was utilized to elucidate the structure-activity relationships. FGE-salmon-PLs strongly inhibited PAF-induced platelet aggregation, while their relevant anti-thrombin effects were at least three times more potent than the previously reported activities of CE-salmon-PLs. TLC-derived lipid fractions corresponding to phosphatidylcholines (PC) and phosphatidylethanolamines (PE) were the most bioactive lipid subclasses obtained, especially against thrombin. Their LC-MS analysis elucidated that they are diacyl- or alkyl-acyl- PC and PE moieties baring ω3 polyunsaturated fatty acids (PUFA) at their sn-2 position, such as eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA). Our results concerning the potent antithrombotic effects of FGE-salmon-PLs against both PAF and thrombin pathways strongly suggest that such food-grade extracts are putative candidates for the development of novel cardioprotective supplements and nutraceuticals. |
format |
Article in Journal/Newspaper |
author |
Alexandros Tsoupras Ronan Lordan Katie Shiels Sushanta Kumar Saha Constantina Nasopoulou Ioannis Zabetakis |
author_facet |
Alexandros Tsoupras Ronan Lordan Katie Shiels Sushanta Kumar Saha Constantina Nasopoulou Ioannis Zabetakis |
author_sort |
Alexandros Tsoupras |
title |
In Vitro Antithrombotic Properties of Salmon (Salmo salar) Phospholipids in a Novel Food-Grade Extract |
title_short |
In Vitro Antithrombotic Properties of Salmon (Salmo salar) Phospholipids in a Novel Food-Grade Extract |
title_full |
In Vitro Antithrombotic Properties of Salmon (Salmo salar) Phospholipids in a Novel Food-Grade Extract |
title_fullStr |
In Vitro Antithrombotic Properties of Salmon (Salmo salar) Phospholipids in a Novel Food-Grade Extract |
title_full_unstemmed |
In Vitro Antithrombotic Properties of Salmon (Salmo salar) Phospholipids in a Novel Food-Grade Extract |
title_sort |
in vitro antithrombotic properties of salmon (salmo salar) phospholipids in a novel food-grade extract |
publisher |
MDPI AG |
publishDate |
2019 |
url |
https://doi.org/10.3390/md17010062 https://doaj.org/article/1e99ce9e07e140daa2297b3ad24b333e |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Marine Drugs, Vol 17, Iss 1, p 62 (2019) |
op_relation |
http://www.mdpi.com/1660-3397/17/1/62 https://doaj.org/toc/1660-3397 1660-3397 doi:10.3390/md17010062 https://doaj.org/article/1e99ce9e07e140daa2297b3ad24b333e |
op_doi |
https://doi.org/10.3390/md17010062 |
container_title |
Marine Drugs |
container_volume |
17 |
container_issue |
1 |
container_start_page |
62 |
_version_ |
1766182479164604416 |