Madurella real-time PCR, a novel approach for eumycetoma diagnosis.
The genus Madurella comprising four species, M. fahalii, M. mycetomatis, M. pseudomycetomatis, and M. tropicana, represents the prevalent cause of eumycetoma worldwide. The four species are phenotypically similar and cause an invariable clinical picture, but differ markedly in their susceptibility t...
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ftdoajarticles:oai:doaj.org/article:1e649db94212483d9dc32a3e88c58780 2023-05-15T15:06:35+02:00 Madurella real-time PCR, a novel approach for eumycetoma diagnosis. Amir Arastehfar Wilson Lim Farnaz Daneshnia Wendy W J van de Sande Ahmed H Fahal Marie Desnos-Ollivier Gerrit S de Hoog Teun Boekhout Sarah A Ahmed 2020-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007845 https://doaj.org/article/1e649db94212483d9dc32a3e88c58780 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007845 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007845 https://doaj.org/article/1e649db94212483d9dc32a3e88c58780 PLoS Neglected Tropical Diseases, Vol 14, Iss 1, p e0007845 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007845 2022-12-31T12:51:32Z The genus Madurella comprising four species, M. fahalii, M. mycetomatis, M. pseudomycetomatis, and M. tropicana, represents the prevalent cause of eumycetoma worldwide. The four species are phenotypically similar and cause an invariable clinical picture, but differ markedly in their susceptibility to antifungal drugs, and epidemiological pattern. Therefore, specific identification is required for optimal management of Madurella infection and to reveal proper epidemiology of the species. In this study, a novel multiplex real-time PCR targeting the four Madurella species was developed and standardized. Evaluation of the assay using reference strains of the target and non-target species resulted in 100% specificity, high analytical reproducibility (R2 values >0.99) and a lowest detection limit of 3 pg target DNA. The accuracy of the real-time PCR was further assessed using biopsies from eumycetoma suspected patients. Unlike culture and DNA sequencing as gold standard diagnostic methods, the real-time PCR yielded accurate diagnosis with specific identification of the causative species in three hours compared to one or two weeks required for culture. The novel method reduces turnaround time as well as labor intensity and high costs associated with current reference methods. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 1 e0007845 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Amir Arastehfar Wilson Lim Farnaz Daneshnia Wendy W J van de Sande Ahmed H Fahal Marie Desnos-Ollivier Gerrit S de Hoog Teun Boekhout Sarah A Ahmed Madurella real-time PCR, a novel approach for eumycetoma diagnosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The genus Madurella comprising four species, M. fahalii, M. mycetomatis, M. pseudomycetomatis, and M. tropicana, represents the prevalent cause of eumycetoma worldwide. The four species are phenotypically similar and cause an invariable clinical picture, but differ markedly in their susceptibility to antifungal drugs, and epidemiological pattern. Therefore, specific identification is required for optimal management of Madurella infection and to reveal proper epidemiology of the species. In this study, a novel multiplex real-time PCR targeting the four Madurella species was developed and standardized. Evaluation of the assay using reference strains of the target and non-target species resulted in 100% specificity, high analytical reproducibility (R2 values >0.99) and a lowest detection limit of 3 pg target DNA. The accuracy of the real-time PCR was further assessed using biopsies from eumycetoma suspected patients. Unlike culture and DNA sequencing as gold standard diagnostic methods, the real-time PCR yielded accurate diagnosis with specific identification of the causative species in three hours compared to one or two weeks required for culture. The novel method reduces turnaround time as well as labor intensity and high costs associated with current reference methods. |
format |
Article in Journal/Newspaper |
author |
Amir Arastehfar Wilson Lim Farnaz Daneshnia Wendy W J van de Sande Ahmed H Fahal Marie Desnos-Ollivier Gerrit S de Hoog Teun Boekhout Sarah A Ahmed |
author_facet |
Amir Arastehfar Wilson Lim Farnaz Daneshnia Wendy W J van de Sande Ahmed H Fahal Marie Desnos-Ollivier Gerrit S de Hoog Teun Boekhout Sarah A Ahmed |
author_sort |
Amir Arastehfar |
title |
Madurella real-time PCR, a novel approach for eumycetoma diagnosis. |
title_short |
Madurella real-time PCR, a novel approach for eumycetoma diagnosis. |
title_full |
Madurella real-time PCR, a novel approach for eumycetoma diagnosis. |
title_fullStr |
Madurella real-time PCR, a novel approach for eumycetoma diagnosis. |
title_full_unstemmed |
Madurella real-time PCR, a novel approach for eumycetoma diagnosis. |
title_sort |
madurella real-time pcr, a novel approach for eumycetoma diagnosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0007845 https://doaj.org/article/1e649db94212483d9dc32a3e88c58780 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 1, p e0007845 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007845 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007845 https://doaj.org/article/1e649db94212483d9dc32a3e88c58780 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007845 |
container_title |
PLOS Neglected Tropical Diseases |
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14 |
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1 |
container_start_page |
e0007845 |
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