Phytochemical studies and anti-ulcerative colitis effect of Moringa oleifera seeds and Egyptian propolis methanol extracts in a rat model
Objective: To analyze the phytochemical constituents, and to explore potential protective effect of the methanol extract of Moringa oleifera (M. oleifera) seeds and Egyptian propolis, each alone or concurrently administered on acetic acid-induced ulcerative colitis in rats. Methods: Eight groups of...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:1e5bc7594be94503837d27403d28b024 2023-05-15T15:12:46+02:00 Phytochemical studies and anti-ulcerative colitis effect of Moringa oleifera seeds and Egyptian propolis methanol extracts in a rat model Attia H Atta Samar M Mouneir Soad M Nasr Doaa Sedky Amany M Mohamed Shimaa A Atta Hassan Mohamed Desouky 2019-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.254603 https://doaj.org/article/1e5bc7594be94503837d27403d28b024 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=3;spage=98;epage=108;aulast=Atta https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.254603 https://doaj.org/article/1e5bc7594be94503837d27403d28b024 Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 3, Pp 98-108 (2019) moringa oleifera seeds propolis ulcerative colitis phytochemical Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2019 ftdoajarticles https://doi.org/10.4103/2221-1691.254603 2022-12-31T09:24:05Z Objective: To analyze the phytochemical constituents, and to explore potential protective effect of the methanol extract of Moringa oleifera (M. oleifera) seeds and Egyptian propolis, each alone or concurrently administered on acetic acid-induced ulcerative colitis in rats. Methods: Eight groups of 5 rats each were used: normal control group with distilled water, model group, two groups with M. oleifera seeds (100 and 200 mg/kg), two groups with propolis (50 and 100 mg/kg), one group with concurrent administration of both, and one group with prednisolone (reference drug). Macro-and microscopic picture, ulcer index and lesion scores, oxidative markers, inflammatory mediators, in vitro activity of the inflammatory enzymes and 1, 1-diphenyl-2-picrylhydrazyl free radicals scavenging activity were evaluated. The phytochemical constituents of both extracts were explored by GC-MS analysis. Results: Both treatments modulated the macro-and microscopic picture, decreased the ulcerative index, lesion score, oxidative markers and inflammatory mediators, and inhibited the COX-1 and COX-2 enzymes. Propolis appeared to be powerful free radicals scavenger. A powerful synergistic effect of both treatments in modulating the course of the disease was reported. GC-MS analysis of methanol extract of M. oleifera seeds and propolis revealed the presence of 50 and 34 compounds, respectively. Conclusions: M. oleifera seeds and propolis methanol extracts have modulated the course of acetic acid-induced ulcerative colitis. Moreover, both treatments induce a good synergistic effect against the disease. Isolation of the active constituents is recommended. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 9 3 98 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
moringa oleifera seeds propolis ulcerative colitis phytochemical Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
moringa oleifera seeds propolis ulcerative colitis phytochemical Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Attia H Atta Samar M Mouneir Soad M Nasr Doaa Sedky Amany M Mohamed Shimaa A Atta Hassan Mohamed Desouky Phytochemical studies and anti-ulcerative colitis effect of Moringa oleifera seeds and Egyptian propolis methanol extracts in a rat model |
topic_facet |
moringa oleifera seeds propolis ulcerative colitis phytochemical Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To analyze the phytochemical constituents, and to explore potential protective effect of the methanol extract of Moringa oleifera (M. oleifera) seeds and Egyptian propolis, each alone or concurrently administered on acetic acid-induced ulcerative colitis in rats. Methods: Eight groups of 5 rats each were used: normal control group with distilled water, model group, two groups with M. oleifera seeds (100 and 200 mg/kg), two groups with propolis (50 and 100 mg/kg), one group with concurrent administration of both, and one group with prednisolone (reference drug). Macro-and microscopic picture, ulcer index and lesion scores, oxidative markers, inflammatory mediators, in vitro activity of the inflammatory enzymes and 1, 1-diphenyl-2-picrylhydrazyl free radicals scavenging activity were evaluated. The phytochemical constituents of both extracts were explored by GC-MS analysis. Results: Both treatments modulated the macro-and microscopic picture, decreased the ulcerative index, lesion score, oxidative markers and inflammatory mediators, and inhibited the COX-1 and COX-2 enzymes. Propolis appeared to be powerful free radicals scavenger. A powerful synergistic effect of both treatments in modulating the course of the disease was reported. GC-MS analysis of methanol extract of M. oleifera seeds and propolis revealed the presence of 50 and 34 compounds, respectively. Conclusions: M. oleifera seeds and propolis methanol extracts have modulated the course of acetic acid-induced ulcerative colitis. Moreover, both treatments induce a good synergistic effect against the disease. Isolation of the active constituents is recommended. |
format |
Article in Journal/Newspaper |
author |
Attia H Atta Samar M Mouneir Soad M Nasr Doaa Sedky Amany M Mohamed Shimaa A Atta Hassan Mohamed Desouky |
author_facet |
Attia H Atta Samar M Mouneir Soad M Nasr Doaa Sedky Amany M Mohamed Shimaa A Atta Hassan Mohamed Desouky |
author_sort |
Attia H Atta |
title |
Phytochemical studies and anti-ulcerative colitis effect of Moringa oleifera seeds and Egyptian propolis methanol extracts in a rat model |
title_short |
Phytochemical studies and anti-ulcerative colitis effect of Moringa oleifera seeds and Egyptian propolis methanol extracts in a rat model |
title_full |
Phytochemical studies and anti-ulcerative colitis effect of Moringa oleifera seeds and Egyptian propolis methanol extracts in a rat model |
title_fullStr |
Phytochemical studies and anti-ulcerative colitis effect of Moringa oleifera seeds and Egyptian propolis methanol extracts in a rat model |
title_full_unstemmed |
Phytochemical studies and anti-ulcerative colitis effect of Moringa oleifera seeds and Egyptian propolis methanol extracts in a rat model |
title_sort |
phytochemical studies and anti-ulcerative colitis effect of moringa oleifera seeds and egyptian propolis methanol extracts in a rat model |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/2221-1691.254603 https://doaj.org/article/1e5bc7594be94503837d27403d28b024 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 9, Iss 3, Pp 98-108 (2019) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2019;volume=9;issue=3;spage=98;epage=108;aulast=Atta https://doaj.org/toc/2221-1691 2221-1691 doi:10.4103/2221-1691.254603 https://doaj.org/article/1e5bc7594be94503837d27403d28b024 |
op_doi |
https://doi.org/10.4103/2221-1691.254603 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
9 |
container_issue |
3 |
container_start_page |
98 |
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1766343402460282880 |