Essential oil from Saussurea costus inhibits proliferation and migration of Eca109 cells via mitochondrial apoptosis and STAT3 signaling
Objective: To investigate the effect and its underlying molecular mechanisms of essential oil from Saussurea costus in esophageal cancer cell line Eca109. Methods: The chemical composition of essential oil from Saussurea costus was investigated by gas chromatography-mass spectrometry (GC-MS). The an...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2022
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ftdoajarticles:oai:doaj.org/article:1e50da5b45194eb69d488938425cafc4 2023-05-15T15:10:38+02:00 Essential oil from Saussurea costus inhibits proliferation and migration of Eca109 cells via mitochondrial apoptosis and STAT3 signaling Xia-Fen Hu Wan-Xin Liu Ren Zhang Wei Zhang Chao Wang Meng Chen Rong Shu Xin-Zhou Yang Qiang Wang 2022-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.345517 https://doaj.org/article/1e50da5b45194eb69d488938425cafc4 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=6;spage=253;epage=261;aulast=Hu https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.345517 https://doaj.org/article/1e50da5b45194eb69d488938425cafc4 Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 6, Pp 253-261 (2022) saussurea costus essential oil gas chromatography- mass spectrometry apoptosis mitochondrial pathway stat3 esophageal cancer anti-proliferation anti-migration eca109 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.4103/2221-1691.345517 2022-12-30T21:37:01Z Objective: To investigate the effect and its underlying molecular mechanisms of essential oil from Saussurea costus in esophageal cancer cell line Eca109. Methods: The chemical composition of essential oil from Saussurea costus was investigated by gas chromatography-mass spectrometry (GC-MS). The anti-proliferative, anti-migrative, and apoptotic effects of essential oil from Saussurea costus against Eca109 cells were analyzed. Moreover, the expression of proteins associated with cell cycle, metastasis, and apoptosis was determined. Results: GC-MS analysis showed that essential oil from Saussurea costus was predominantly comprised of sesquiterpenes. Saussurea costus essential oil inhibited the viability of Eca109 cells in a dose-and time-dependent manner with IC50 values of (24.29±1.49), (19.16±2.27) and (6.97±0.86) μg/mL at 12, 24, and 48 h, respectively. The expression levels of target proteins in the cell cycle (phase G1/S), including cyclin D1, p21, and p53, were affected by Saussurea costus essential oil. The essential oil also downregulated the expression of metastasis-related proteins MMP-9 and MMP-2. Moreover, it induced apoptosis of Eca109 cells through the mitochondrial pathway, as well as inhibition of STAT3 phosphorylation. Conclusions: The essential oil from Saussurea costus exhibited anti-proliferative, anti-migrative, and apoptotic effects on Eca109 cells, and could be further explored as a potential anti-esophageal cancer agent. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 12 6 253 |
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ftdoajarticles |
language |
English |
topic |
saussurea costus essential oil gas chromatography- mass spectrometry apoptosis mitochondrial pathway stat3 esophageal cancer anti-proliferation anti-migration eca109 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
saussurea costus essential oil gas chromatography- mass spectrometry apoptosis mitochondrial pathway stat3 esophageal cancer anti-proliferation anti-migration eca109 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Xia-Fen Hu Wan-Xin Liu Ren Zhang Wei Zhang Chao Wang Meng Chen Rong Shu Xin-Zhou Yang Qiang Wang Essential oil from Saussurea costus inhibits proliferation and migration of Eca109 cells via mitochondrial apoptosis and STAT3 signaling |
topic_facet |
saussurea costus essential oil gas chromatography- mass spectrometry apoptosis mitochondrial pathway stat3 esophageal cancer anti-proliferation anti-migration eca109 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the effect and its underlying molecular mechanisms of essential oil from Saussurea costus in esophageal cancer cell line Eca109. Methods: The chemical composition of essential oil from Saussurea costus was investigated by gas chromatography-mass spectrometry (GC-MS). The anti-proliferative, anti-migrative, and apoptotic effects of essential oil from Saussurea costus against Eca109 cells were analyzed. Moreover, the expression of proteins associated with cell cycle, metastasis, and apoptosis was determined. Results: GC-MS analysis showed that essential oil from Saussurea costus was predominantly comprised of sesquiterpenes. Saussurea costus essential oil inhibited the viability of Eca109 cells in a dose-and time-dependent manner with IC50 values of (24.29±1.49), (19.16±2.27) and (6.97±0.86) μg/mL at 12, 24, and 48 h, respectively. The expression levels of target proteins in the cell cycle (phase G1/S), including cyclin D1, p21, and p53, were affected by Saussurea costus essential oil. The essential oil also downregulated the expression of metastasis-related proteins MMP-9 and MMP-2. Moreover, it induced apoptosis of Eca109 cells through the mitochondrial pathway, as well as inhibition of STAT3 phosphorylation. Conclusions: The essential oil from Saussurea costus exhibited anti-proliferative, anti-migrative, and apoptotic effects on Eca109 cells, and could be further explored as a potential anti-esophageal cancer agent. |
format |
Article in Journal/Newspaper |
author |
Xia-Fen Hu Wan-Xin Liu Ren Zhang Wei Zhang Chao Wang Meng Chen Rong Shu Xin-Zhou Yang Qiang Wang |
author_facet |
Xia-Fen Hu Wan-Xin Liu Ren Zhang Wei Zhang Chao Wang Meng Chen Rong Shu Xin-Zhou Yang Qiang Wang |
author_sort |
Xia-Fen Hu |
title |
Essential oil from Saussurea costus inhibits proliferation and migration of Eca109 cells via mitochondrial apoptosis and STAT3 signaling |
title_short |
Essential oil from Saussurea costus inhibits proliferation and migration of Eca109 cells via mitochondrial apoptosis and STAT3 signaling |
title_full |
Essential oil from Saussurea costus inhibits proliferation and migration of Eca109 cells via mitochondrial apoptosis and STAT3 signaling |
title_fullStr |
Essential oil from Saussurea costus inhibits proliferation and migration of Eca109 cells via mitochondrial apoptosis and STAT3 signaling |
title_full_unstemmed |
Essential oil from Saussurea costus inhibits proliferation and migration of Eca109 cells via mitochondrial apoptosis and STAT3 signaling |
title_sort |
essential oil from saussurea costus inhibits proliferation and migration of eca109 cells via mitochondrial apoptosis and stat3 signaling |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2022 |
url |
https://doi.org/10.4103/2221-1691.345517 https://doaj.org/article/1e50da5b45194eb69d488938425cafc4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 6, Pp 253-261 (2022) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=6;spage=253;epage=261;aulast=Hu https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.345517 https://doaj.org/article/1e50da5b45194eb69d488938425cafc4 |
op_doi |
https://doi.org/10.4103/2221-1691.345517 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
12 |
container_issue |
6 |
container_start_page |
253 |
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1766341630113087488 |