PAH- and PCB-induced Alterations of Protein Tyrosine Kinase and Cytokine Gene Transcription in Harbor Seal (Phoca Vitulina) PBMC
Mechanisms underlying in vitro immunomodulatory effects of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) were investigated in harbor seal peripheral leukocytes, via real-time PCR. We examined the relative genetic expression of the protein tyrosine kinases (PTKs) Fyn an...
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ftdoajarticles:oai:doaj.org/article:1e1e1cf564de423083839d306827cb75 2024-09-15T18:10:38+00:00 PAH- and PCB-induced Alterations of Protein Tyrosine Kinase and Cytokine Gene Transcription in Harbor Seal (Phoca Vitulina) PBMC Jennifer C. C. Neale Thomas P. Kenny Ronald S. Tjeerdema M. Eric Gershwin 2005-01-01T00:00:00Z https://doi.org/10.1080/17402520500116624 https://doaj.org/article/1e1e1cf564de423083839d306827cb75 EN eng Wiley http://dx.doi.org/10.1080/17402520500116624 https://doaj.org/toc/1740-2522 https://doaj.org/toc/1740-2530 1740-2522 1740-2530 doi:10.1080/17402520500116624 https://doaj.org/article/1e1e1cf564de423083839d306827cb75 Clinical and Developmental Immunology, Vol 12, Iss 2, Pp 91-97 (2005) Immunologic diseases. Allergy RC581-607 article 2005 ftdoajarticles https://doi.org/10.1080/17402520500116624 2024-08-05T17:48:36Z Mechanisms underlying in vitro immunomodulatory effects of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) were investigated in harbor seal peripheral leukocytes, via real-time PCR. We examined the relative genetic expression of the protein tyrosine kinases (PTKs) Fyn and Itk, which play a critical role in T cell activation, and IL-2, a cytokine of central importance in initiating adaptive immune responses. IL-1, the macrophage-derived pro-inflammatory cytokine of innate immunity, was also included as a measure of macrophage function. Harbor seal PBMC were exposed to the prototypic immunotoxic PAH benzo[a]pyrene (BaP), 3,3',4,4',5,5'-hexachlorobiphenyl (CB-169), a model immunotoxic PCB, or DMSO (vehicle control). Exposure of Con A-stimulated harbor seal PBMC to both BaP and CB-169 produced significantly altered expression in all four targets relative to vehicle controls. The PTKs Fyn and Itk were both up-regulated following exposure to BaP and CB-169. In contrast, transcripts for IL-2 and IL-1 were decreased relative to controls by both treatments. Our findings are consistent with those of previous researchers working with human and rodent systems and support a hypothesis of contaminant-altered lymphocyte function mediated (at least in part) by disruption of T cell receptor (TCR) signaling and cytokine production. Article in Journal/Newspaper harbor seal Phoca vitulina Directory of Open Access Journals: DOAJ Articles Clinical and Developmental Immunology 12 2 91 97 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Immunologic diseases. Allergy RC581-607 |
spellingShingle |
Immunologic diseases. Allergy RC581-607 Jennifer C. C. Neale Thomas P. Kenny Ronald S. Tjeerdema M. Eric Gershwin PAH- and PCB-induced Alterations of Protein Tyrosine Kinase and Cytokine Gene Transcription in Harbor Seal (Phoca Vitulina) PBMC |
topic_facet |
Immunologic diseases. Allergy RC581-607 |
description |
Mechanisms underlying in vitro immunomodulatory effects of polycyclic aromatic hydrocarbons (PAHs) and polychlorinated biphenyls (PCBs) were investigated in harbor seal peripheral leukocytes, via real-time PCR. We examined the relative genetic expression of the protein tyrosine kinases (PTKs) Fyn and Itk, which play a critical role in T cell activation, and IL-2, a cytokine of central importance in initiating adaptive immune responses. IL-1, the macrophage-derived pro-inflammatory cytokine of innate immunity, was also included as a measure of macrophage function. Harbor seal PBMC were exposed to the prototypic immunotoxic PAH benzo[a]pyrene (BaP), 3,3',4,4',5,5'-hexachlorobiphenyl (CB-169), a model immunotoxic PCB, or DMSO (vehicle control). Exposure of Con A-stimulated harbor seal PBMC to both BaP and CB-169 produced significantly altered expression in all four targets relative to vehicle controls. The PTKs Fyn and Itk were both up-regulated following exposure to BaP and CB-169. In contrast, transcripts for IL-2 and IL-1 were decreased relative to controls by both treatments. Our findings are consistent with those of previous researchers working with human and rodent systems and support a hypothesis of contaminant-altered lymphocyte function mediated (at least in part) by disruption of T cell receptor (TCR) signaling and cytokine production. |
format |
Article in Journal/Newspaper |
author |
Jennifer C. C. Neale Thomas P. Kenny Ronald S. Tjeerdema M. Eric Gershwin |
author_facet |
Jennifer C. C. Neale Thomas P. Kenny Ronald S. Tjeerdema M. Eric Gershwin |
author_sort |
Jennifer C. C. Neale |
title |
PAH- and PCB-induced Alterations of Protein Tyrosine Kinase and Cytokine Gene Transcription in Harbor Seal (Phoca Vitulina) PBMC |
title_short |
PAH- and PCB-induced Alterations of Protein Tyrosine Kinase and Cytokine Gene Transcription in Harbor Seal (Phoca Vitulina) PBMC |
title_full |
PAH- and PCB-induced Alterations of Protein Tyrosine Kinase and Cytokine Gene Transcription in Harbor Seal (Phoca Vitulina) PBMC |
title_fullStr |
PAH- and PCB-induced Alterations of Protein Tyrosine Kinase and Cytokine Gene Transcription in Harbor Seal (Phoca Vitulina) PBMC |
title_full_unstemmed |
PAH- and PCB-induced Alterations of Protein Tyrosine Kinase and Cytokine Gene Transcription in Harbor Seal (Phoca Vitulina) PBMC |
title_sort |
pah- and pcb-induced alterations of protein tyrosine kinase and cytokine gene transcription in harbor seal (phoca vitulina) pbmc |
publisher |
Wiley |
publishDate |
2005 |
url |
https://doi.org/10.1080/17402520500116624 https://doaj.org/article/1e1e1cf564de423083839d306827cb75 |
genre |
harbor seal Phoca vitulina |
genre_facet |
harbor seal Phoca vitulina |
op_source |
Clinical and Developmental Immunology, Vol 12, Iss 2, Pp 91-97 (2005) |
op_relation |
http://dx.doi.org/10.1080/17402520500116624 https://doaj.org/toc/1740-2522 https://doaj.org/toc/1740-2530 1740-2522 1740-2530 doi:10.1080/17402520500116624 https://doaj.org/article/1e1e1cf564de423083839d306827cb75 |
op_doi |
https://doi.org/10.1080/17402520500116624 |
container_title |
Clinical and Developmental Immunology |
container_volume |
12 |
container_issue |
2 |
container_start_page |
91 |
op_container_end_page |
97 |
_version_ |
1810448224705576960 |