Monitoring antigenic variations of enterovirus 71: implications for virus surveillance and vaccine development.
Enterovirus 71 (EV71) causes life-threatening epidemics in Asia and can be phylogenetically classified into three major genogroups (A ∼ C) including 11 genotypes (A, B1 ∼ B5, and C1 ∼ C5). Recently, EV71 epidemics occurred cyclically in Taiwan with different genotypes. In recent years, human studies...
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ftdoajarticles:oai:doaj.org/article:1dfbeca1ba534a049266cd7f8740799f 2023-05-15T15:10:30+02:00 Monitoring antigenic variations of enterovirus 71: implications for virus surveillance and vaccine development. Min-Yuan Chia Wan-Yu Chung Pai-Shan Chiang Yeh-Sheng Chien Mei-Shang Ho Min-Shi Lee 2014-07-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003044 https://doaj.org/article/1dfbeca1ba534a049266cd7f8740799f EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4109910?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003044 https://doaj.org/article/1dfbeca1ba534a049266cd7f8740799f PLoS Neglected Tropical Diseases, Vol 8, Iss 7, p e3044 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003044 2022-12-31T14:27:54Z Enterovirus 71 (EV71) causes life-threatening epidemics in Asia and can be phylogenetically classified into three major genogroups (A ∼ C) including 11 genotypes (A, B1 ∼ B5, and C1 ∼ C5). Recently, EV71 epidemics occurred cyclically in Taiwan with different genotypes. In recent years, human studies using post-infection sera obtained from children have detected antigenic variations among different EV71 strains. Therefore, surveillance of enterovirus 71 should include phylogenetic and antigenic analysis. Due to limitation of sera available from children with EV71 primary infection, suitable animal models should be developed to generate a panel of antisera for monitoring EV71 antigenic variations. Twelve reference strains representing the 11 EV71 genotypes were grown in rhabdomyosarcoma cells. Infectious EV71 particles were purified and collected to immunize rabbits. The rabbit antisera were then employed to measure neutralizing antibody titers against the 12 reference strains and 5 recent strains. Rabbits immunized with genogroup B and C viruses consistently have a lower neutralizing antibody titers against genogroup A (≧ 8-fold difference) and antigenic variations between genogroup B and C viruses can be detected but did not have a clear pattern, which are consistent with previous human studies. Comparison between human and rabbit neutralizing antibody profiles, the results showed that ≧ 8-fold difference in rabbit cross-reactive antibody ratios could be used to screen EV71 isolates for identifying potential antigenic variants. In conclusion, a rabbit model was developed to monitor antigenic variations of EV71, which are critical to select vaccine strains and predict epidemics. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 7 e3044 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Min-Yuan Chia Wan-Yu Chung Pai-Shan Chiang Yeh-Sheng Chien Mei-Shang Ho Min-Shi Lee Monitoring antigenic variations of enterovirus 71: implications for virus surveillance and vaccine development. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Enterovirus 71 (EV71) causes life-threatening epidemics in Asia and can be phylogenetically classified into three major genogroups (A ∼ C) including 11 genotypes (A, B1 ∼ B5, and C1 ∼ C5). Recently, EV71 epidemics occurred cyclically in Taiwan with different genotypes. In recent years, human studies using post-infection sera obtained from children have detected antigenic variations among different EV71 strains. Therefore, surveillance of enterovirus 71 should include phylogenetic and antigenic analysis. Due to limitation of sera available from children with EV71 primary infection, suitable animal models should be developed to generate a panel of antisera for monitoring EV71 antigenic variations. Twelve reference strains representing the 11 EV71 genotypes were grown in rhabdomyosarcoma cells. Infectious EV71 particles were purified and collected to immunize rabbits. The rabbit antisera were then employed to measure neutralizing antibody titers against the 12 reference strains and 5 recent strains. Rabbits immunized with genogroup B and C viruses consistently have a lower neutralizing antibody titers against genogroup A (≧ 8-fold difference) and antigenic variations between genogroup B and C viruses can be detected but did not have a clear pattern, which are consistent with previous human studies. Comparison between human and rabbit neutralizing antibody profiles, the results showed that ≧ 8-fold difference in rabbit cross-reactive antibody ratios could be used to screen EV71 isolates for identifying potential antigenic variants. In conclusion, a rabbit model was developed to monitor antigenic variations of EV71, which are critical to select vaccine strains and predict epidemics. |
format |
Article in Journal/Newspaper |
author |
Min-Yuan Chia Wan-Yu Chung Pai-Shan Chiang Yeh-Sheng Chien Mei-Shang Ho Min-Shi Lee |
author_facet |
Min-Yuan Chia Wan-Yu Chung Pai-Shan Chiang Yeh-Sheng Chien Mei-Shang Ho Min-Shi Lee |
author_sort |
Min-Yuan Chia |
title |
Monitoring antigenic variations of enterovirus 71: implications for virus surveillance and vaccine development. |
title_short |
Monitoring antigenic variations of enterovirus 71: implications for virus surveillance and vaccine development. |
title_full |
Monitoring antigenic variations of enterovirus 71: implications for virus surveillance and vaccine development. |
title_fullStr |
Monitoring antigenic variations of enterovirus 71: implications for virus surveillance and vaccine development. |
title_full_unstemmed |
Monitoring antigenic variations of enterovirus 71: implications for virus surveillance and vaccine development. |
title_sort |
monitoring antigenic variations of enterovirus 71: implications for virus surveillance and vaccine development. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003044 https://doaj.org/article/1dfbeca1ba534a049266cd7f8740799f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 7, p e3044 (2014) |
op_relation |
http://europepmc.org/articles/PMC4109910?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003044 https://doaj.org/article/1dfbeca1ba534a049266cd7f8740799f |
op_doi |
https://doi.org/10.1371/journal.pntd.0003044 |
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PLoS Neglected Tropical Diseases |
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8 |
container_issue |
7 |
container_start_page |
e3044 |
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