Transcriptome Sequencing Reveals Large-Scale Changes in Axenic Aedes aegypti Larvae.
Mosquitoes host communities of microbes in their digestive tract that consist primarily of bacteria. We previously reported that Aedes aegypti larvae colonized by a native community of bacteria and gnotobiotic larvae colonized by only Escherichia coli develop very similarly into adults, whereas axen...
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ftdoajarticles:oai:doaj.org/article:1ddd12716a694531a0b6314750252b7b 2023-05-15T15:09:38+02:00 Transcriptome Sequencing Reveals Large-Scale Changes in Axenic Aedes aegypti Larvae. Kevin J Vogel Luca Valzania Kerri L Coon Mark R Brown Michael R Strand 2017-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005273 https://doaj.org/article/1ddd12716a694531a0b6314750252b7b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5245907?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005273 https://doaj.org/article/1ddd12716a694531a0b6314750252b7b PLoS Neglected Tropical Diseases, Vol 11, Iss 1, p e0005273 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005273 2022-12-31T01:35:10Z Mosquitoes host communities of microbes in their digestive tract that consist primarily of bacteria. We previously reported that Aedes aegypti larvae colonized by a native community of bacteria and gnotobiotic larvae colonized by only Escherichia coli develop very similarly into adults, whereas axenic larvae never molt and die as first instars. In this study, we extended these findings by first comparing the growth and abundance of bacteria in conventional, gnotobiotic, and axenic larvae during the first instar. Results showed that conventional and gnotobiotic larvae exhibited no differences in growth, timing of molting, or number of bacteria in their digestive tract. Axenic larvae in contrast grew minimally and never achieved the critical size associated with molting by conventional and gnotobiotic larvae. In the second part of the study we compared patterns of gene expression in conventional, gnotobiotic and axenic larvae by conducting an RNAseq analysis of gut and nongut tissues (carcass) at 22 h post-hatching. Approximately 12% of Ae. aegypti transcripts were differentially expressed in axenic versus conventional or gnotobiotic larvae. However, this profile consisted primarily of transcripts in seven categories that included the down-regulation of select peptidases in the gut and up-regulation of several genes in the gut and carcass with roles in amino acid transport, hormonal signaling, and metabolism. Overall, our results indicate that axenic larvae exhibit alterations in gene expression consistent with defects in acquisition and assimilation of nutrients required for growth. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 1 e0005273 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Kevin J Vogel Luca Valzania Kerri L Coon Mark R Brown Michael R Strand Transcriptome Sequencing Reveals Large-Scale Changes in Axenic Aedes aegypti Larvae. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Mosquitoes host communities of microbes in their digestive tract that consist primarily of bacteria. We previously reported that Aedes aegypti larvae colonized by a native community of bacteria and gnotobiotic larvae colonized by only Escherichia coli develop very similarly into adults, whereas axenic larvae never molt and die as first instars. In this study, we extended these findings by first comparing the growth and abundance of bacteria in conventional, gnotobiotic, and axenic larvae during the first instar. Results showed that conventional and gnotobiotic larvae exhibited no differences in growth, timing of molting, or number of bacteria in their digestive tract. Axenic larvae in contrast grew minimally and never achieved the critical size associated with molting by conventional and gnotobiotic larvae. In the second part of the study we compared patterns of gene expression in conventional, gnotobiotic and axenic larvae by conducting an RNAseq analysis of gut and nongut tissues (carcass) at 22 h post-hatching. Approximately 12% of Ae. aegypti transcripts were differentially expressed in axenic versus conventional or gnotobiotic larvae. However, this profile consisted primarily of transcripts in seven categories that included the down-regulation of select peptidases in the gut and up-regulation of several genes in the gut and carcass with roles in amino acid transport, hormonal signaling, and metabolism. Overall, our results indicate that axenic larvae exhibit alterations in gene expression consistent with defects in acquisition and assimilation of nutrients required for growth. |
format |
Article in Journal/Newspaper |
author |
Kevin J Vogel Luca Valzania Kerri L Coon Mark R Brown Michael R Strand |
author_facet |
Kevin J Vogel Luca Valzania Kerri L Coon Mark R Brown Michael R Strand |
author_sort |
Kevin J Vogel |
title |
Transcriptome Sequencing Reveals Large-Scale Changes in Axenic Aedes aegypti Larvae. |
title_short |
Transcriptome Sequencing Reveals Large-Scale Changes in Axenic Aedes aegypti Larvae. |
title_full |
Transcriptome Sequencing Reveals Large-Scale Changes in Axenic Aedes aegypti Larvae. |
title_fullStr |
Transcriptome Sequencing Reveals Large-Scale Changes in Axenic Aedes aegypti Larvae. |
title_full_unstemmed |
Transcriptome Sequencing Reveals Large-Scale Changes in Axenic Aedes aegypti Larvae. |
title_sort |
transcriptome sequencing reveals large-scale changes in axenic aedes aegypti larvae. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005273 https://doaj.org/article/1ddd12716a694531a0b6314750252b7b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 1, p e0005273 (2017) |
op_relation |
http://europepmc.org/articles/PMC5245907?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005273 https://doaj.org/article/1ddd12716a694531a0b6314750252b7b |
op_doi |
https://doi.org/10.1371/journal.pntd.0005273 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
1 |
container_start_page |
e0005273 |
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1766340797051961344 |