Alterations in urine, serum and brain metabolomic profiles exhibit sexual dimorphism during malaria disease progression
Abstract Background Metabolic changes in the host in response to Plasmodium infection play a crucial role in the pathogenesis of malaria. Alterations in metabolism of male and female mice infected with Plasmodium berghei ANKA are reported here. Methods 1 H NMR spectra of urine, sera and brain extrac...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2010
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-9-110 https://doaj.org/article/1dd943e4b1d24af0bc2e6804d0d86725 |
id |
ftdoajarticles:oai:doaj.org/article:1dd943e4b1d24af0bc2e6804d0d86725 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:1dd943e4b1d24af0bc2e6804d0d86725 2023-05-15T15:15:12+02:00 Alterations in urine, serum and brain metabolomic profiles exhibit sexual dimorphism during malaria disease progression Sharma Shobhona Rege Mayuri Basant Angika Sonawat Haripalsingh M 2010-04-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-110 https://doaj.org/article/1dd943e4b1d24af0bc2e6804d0d86725 EN eng BMC http://www.malariajournal.com/content/9/1/110 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-110 1475-2875 https://doaj.org/article/1dd943e4b1d24af0bc2e6804d0d86725 Malaria Journal, Vol 9, Iss 1, p 110 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-110 2022-12-31T13:47:40Z Abstract Background Metabolic changes in the host in response to Plasmodium infection play a crucial role in the pathogenesis of malaria. Alterations in metabolism of male and female mice infected with Plasmodium berghei ANKA are reported here. Methods 1 H NMR spectra of urine, sera and brain extracts of these mice were analysed over disease progression using Principle Component Analysis and Orthogonal Partial Least Square Discriminant Analysis. Results Analyses of overall changes in urinary profiles during disease progression demonstrate that females show a significant early post-infection shift in metabolism as compared to males. In contrast, serum profiles of female mice remain unaltered in the early infection stages; whereas that of the male mice changed. Brain metabolite profiles do not show global changes in the early stages of infection in either sex. By the late stages urine, serum and brain profiles of both sexes are severely affected. Analyses of individual metabolites show significant increase in lactate, alanine and lysine, kynurenic acid and quinolinic acid in sera of both males and females at this stage. Early changes in female urine are marked by an increase of ureidopropionate, lowering of carnitine and transient enhancement of asparagine and dimethylglycine. Several metabolites when analysed individually in sera and brain reveal significant changes in their levels in the early phase of infection mainly in female mice. Asparagine and dimethylglycine levels decrease and quinolinic acid increases early in sera of infected females. In brain extracts of females, an early rise in levels is also observed for lactate, alanine and glycerol, kynurenic acid, ureidopropionate and 2-hydroxy-2-methylbutyrate. Conclusions These results suggest that P. berghei infection leads to impairment of glycolysis, lipid metabolism, metabolism of tryptophan and degradation of uracil. Characterization of early changes along these pathways may be crucial for prognosis and better disease management. Additionally, the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 1 110 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Sharma Shobhona Rege Mayuri Basant Angika Sonawat Haripalsingh M Alterations in urine, serum and brain metabolomic profiles exhibit sexual dimorphism during malaria disease progression |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Metabolic changes in the host in response to Plasmodium infection play a crucial role in the pathogenesis of malaria. Alterations in metabolism of male and female mice infected with Plasmodium berghei ANKA are reported here. Methods 1 H NMR spectra of urine, sera and brain extracts of these mice were analysed over disease progression using Principle Component Analysis and Orthogonal Partial Least Square Discriminant Analysis. Results Analyses of overall changes in urinary profiles during disease progression demonstrate that females show a significant early post-infection shift in metabolism as compared to males. In contrast, serum profiles of female mice remain unaltered in the early infection stages; whereas that of the male mice changed. Brain metabolite profiles do not show global changes in the early stages of infection in either sex. By the late stages urine, serum and brain profiles of both sexes are severely affected. Analyses of individual metabolites show significant increase in lactate, alanine and lysine, kynurenic acid and quinolinic acid in sera of both males and females at this stage. Early changes in female urine are marked by an increase of ureidopropionate, lowering of carnitine and transient enhancement of asparagine and dimethylglycine. Several metabolites when analysed individually in sera and brain reveal significant changes in their levels in the early phase of infection mainly in female mice. Asparagine and dimethylglycine levels decrease and quinolinic acid increases early in sera of infected females. In brain extracts of females, an early rise in levels is also observed for lactate, alanine and glycerol, kynurenic acid, ureidopropionate and 2-hydroxy-2-methylbutyrate. Conclusions These results suggest that P. berghei infection leads to impairment of glycolysis, lipid metabolism, metabolism of tryptophan and degradation of uracil. Characterization of early changes along these pathways may be crucial for prognosis and better disease management. Additionally, the ... |
format |
Article in Journal/Newspaper |
author |
Sharma Shobhona Rege Mayuri Basant Angika Sonawat Haripalsingh M |
author_facet |
Sharma Shobhona Rege Mayuri Basant Angika Sonawat Haripalsingh M |
author_sort |
Sharma Shobhona |
title |
Alterations in urine, serum and brain metabolomic profiles exhibit sexual dimorphism during malaria disease progression |
title_short |
Alterations in urine, serum and brain metabolomic profiles exhibit sexual dimorphism during malaria disease progression |
title_full |
Alterations in urine, serum and brain metabolomic profiles exhibit sexual dimorphism during malaria disease progression |
title_fullStr |
Alterations in urine, serum and brain metabolomic profiles exhibit sexual dimorphism during malaria disease progression |
title_full_unstemmed |
Alterations in urine, serum and brain metabolomic profiles exhibit sexual dimorphism during malaria disease progression |
title_sort |
alterations in urine, serum and brain metabolomic profiles exhibit sexual dimorphism during malaria disease progression |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-110 https://doaj.org/article/1dd943e4b1d24af0bc2e6804d0d86725 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 110 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/110 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-110 1475-2875 https://doaj.org/article/1dd943e4b1d24af0bc2e6804d0d86725 |
op_doi |
https://doi.org/10.1186/1475-2875-9-110 |
container_title |
Malaria Journal |
container_volume |
9 |
container_issue |
1 |
container_start_page |
110 |
_version_ |
1766345579482316800 |