DNA Methylation and Temperature Stress in an Antarctic Polychaete, Spiophanes tcherniai
Epigenetic modifications of DNA and histones are a primary mechanism by which gene expression activities may be modified in response to environmental stimuli. Here we characterize patterns of methyl-cytosine composition in the marine polychaete emph{Spiophanes tcherniai} from McMurdo Sound, Antarcti...
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ftdoajarticles:oai:doaj.org/article:1dc6c2c7fca2444583b0baebe3e11ab6 2023-05-15T13:56:43+02:00 DNA Methylation and Temperature Stress in an Antarctic Polychaete, Spiophanes tcherniai Adam G. Marsh Annamarie ePasqualone 2014-05-01T00:00:00Z https://doi.org/10.3389/fphys.2014.00173 https://doaj.org/article/1dc6c2c7fca2444583b0baebe3e11ab6 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00173/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2014.00173 https://doaj.org/article/1dc6c2c7fca2444583b0baebe3e11ab6 Frontiers in Physiology, Vol 5 (2014) DNA Methylation Energy Metabolism epigenetics Antarctica cold acclimation polychaete Physiology QP1-981 article 2014 ftdoajarticles https://doi.org/10.3389/fphys.2014.00173 2022-12-31T12:21:22Z Epigenetic modifications of DNA and histones are a primary mechanism by which gene expression activities may be modified in response to environmental stimuli. Here we characterize patterns of methyl-cytosine composition in the marine polychaete emph{Spiophanes tcherniai} from McMurdo Sound, Antarctica. We cultured adult worms at two temperatures, -1.5 C (ambient control) and +4 C (warm treatment), for four weeks. We observed a rapid capacity for emph{S. tcherniai} organismal respiration rates and underlying catalytic rates of citrate synthase to acclimate at +4 C and return to control levels. We profiled changes in the methylation states of CpG sites in these treatments using an NGS strategy to computationally reconstruct and quantify methylation status across the genome. In our analysis we recovered 120,000 CpG sites in assembled contigs from both treatments. Of those, we were able to align 28,000 CpG sites in common between the two sample groups. In comparing these aligned sites between treatments, only 3,000 (11%) evidenced a change in methylation state, but over 85% of changes involved a gain of a 5-methyl group on a CpG site (net increase in methyation). The ability to score CpG sites as partially methylated among gDNA copies in a sample opens up a new avenue for assessing DNA methylation responses to changing environments. By quantitatively distinguishing a ``mixed'' population of copies of one CpG site, we can begin to identify dynamic, non-binary, continuous-response reactions in DNA methylation intensity or density that previously may have been overlooked as noise. Article in Journal/Newspaper Antarc* Antarctic Antarctica McMurdo Sound Directory of Open Access Journals: DOAJ Articles Antarctic McMurdo Sound Frontiers in Physiology 5 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
DNA Methylation Energy Metabolism epigenetics Antarctica cold acclimation polychaete Physiology QP1-981 |
spellingShingle |
DNA Methylation Energy Metabolism epigenetics Antarctica cold acclimation polychaete Physiology QP1-981 Adam G. Marsh Annamarie ePasqualone DNA Methylation and Temperature Stress in an Antarctic Polychaete, Spiophanes tcherniai |
topic_facet |
DNA Methylation Energy Metabolism epigenetics Antarctica cold acclimation polychaete Physiology QP1-981 |
description |
Epigenetic modifications of DNA and histones are a primary mechanism by which gene expression activities may be modified in response to environmental stimuli. Here we characterize patterns of methyl-cytosine composition in the marine polychaete emph{Spiophanes tcherniai} from McMurdo Sound, Antarctica. We cultured adult worms at two temperatures, -1.5 C (ambient control) and +4 C (warm treatment), for four weeks. We observed a rapid capacity for emph{S. tcherniai} organismal respiration rates and underlying catalytic rates of citrate synthase to acclimate at +4 C and return to control levels. We profiled changes in the methylation states of CpG sites in these treatments using an NGS strategy to computationally reconstruct and quantify methylation status across the genome. In our analysis we recovered 120,000 CpG sites in assembled contigs from both treatments. Of those, we were able to align 28,000 CpG sites in common between the two sample groups. In comparing these aligned sites between treatments, only 3,000 (11%) evidenced a change in methylation state, but over 85% of changes involved a gain of a 5-methyl group on a CpG site (net increase in methyation). The ability to score CpG sites as partially methylated among gDNA copies in a sample opens up a new avenue for assessing DNA methylation responses to changing environments. By quantitatively distinguishing a ``mixed'' population of copies of one CpG site, we can begin to identify dynamic, non-binary, continuous-response reactions in DNA methylation intensity or density that previously may have been overlooked as noise. |
format |
Article in Journal/Newspaper |
author |
Adam G. Marsh Annamarie ePasqualone |
author_facet |
Adam G. Marsh Annamarie ePasqualone |
author_sort |
Adam G. Marsh |
title |
DNA Methylation and Temperature Stress in an Antarctic Polychaete, Spiophanes tcherniai |
title_short |
DNA Methylation and Temperature Stress in an Antarctic Polychaete, Spiophanes tcherniai |
title_full |
DNA Methylation and Temperature Stress in an Antarctic Polychaete, Spiophanes tcherniai |
title_fullStr |
DNA Methylation and Temperature Stress in an Antarctic Polychaete, Spiophanes tcherniai |
title_full_unstemmed |
DNA Methylation and Temperature Stress in an Antarctic Polychaete, Spiophanes tcherniai |
title_sort |
dna methylation and temperature stress in an antarctic polychaete, spiophanes tcherniai |
publisher |
Frontiers Media S.A. |
publishDate |
2014 |
url |
https://doi.org/10.3389/fphys.2014.00173 https://doaj.org/article/1dc6c2c7fca2444583b0baebe3e11ab6 |
geographic |
Antarctic McMurdo Sound |
geographic_facet |
Antarctic McMurdo Sound |
genre |
Antarc* Antarctic Antarctica McMurdo Sound |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Sound |
op_source |
Frontiers in Physiology, Vol 5 (2014) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fphys.2014.00173/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2014.00173 https://doaj.org/article/1dc6c2c7fca2444583b0baebe3e11ab6 |
op_doi |
https://doi.org/10.3389/fphys.2014.00173 |
container_title |
Frontiers in Physiology |
container_volume |
5 |
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1766264292049420288 |