Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique
INTRODUCTION: Studies strongly indicate Dientamoeba fragilis as one of the causes of diarrhea in human immunodeficiency virus (HIV) patients. METHODS: The objective of the present study was to evaluate the prevalence of D. fragilis associated with the causes of diarrhea in 82 HIV/ AIDS patients hosp...
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ftdoajarticles:oai:doaj.org/article:1db3f8371f5e440e897ee0d24ed357d1 2023-05-15T15:09:27+02:00 Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique Juliana Alves Garcia Sergio Cimerman 2012-04-01T00:00:00Z https://doi.org/10.1590/S0037-86822012000200003 https://doaj.org/article/1db3f8371f5e440e897ee0d24ed357d1 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000200003&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000200003 https://doaj.org/article/1db3f8371f5e440e897ee0d24ed357d1 Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 2, Pp 156-158 (2012) Dientamoeba fragilis Diarréia AIDS Hematoxilina férrica simplificada Arctic medicine. Tropical medicine RC955-962 article 2012 ftdoajarticles https://doi.org/10.1590/S0037-86822012000200003 2022-12-30T21:37:16Z INTRODUCTION: Studies strongly indicate Dientamoeba fragilis as one of the causes of diarrhea in human immunodeficiency virus (HIV) patients. METHODS: The objective of the present study was to evaluate the prevalence of D. fragilis associated with the causes of diarrhea in 82 HIV/ AIDS patients hospitalized at the Instituto de Infectologia Emílio Ribas from September 2006 to November 2008. RESULTS: In total, 105 samples were collected from 82 patients. Unprotected sex was the most frequent cause of HIV infection (46.3%), followed by the use of injectable or non-injectable drugs (14.6%). Patients presented with viral loads of 49-750,000 copies/ mL (average: 73,849 ± 124,850 copies/mL) and CD4 counts ranging of 2-1,306 cells/mm³ (average: 159 ± 250 cells/mm³). On an average, the odds of obtaining a positive result by using the other techniques (Hoffman, Pons and Janer or Lutz; Ritchie) were 2.7 times higher than the chance of obtaining a positive result by using the simplified iron hematoxylin method. Significant differences were found between the methods (p = 0.003). CONCLUSIONS: The other techniques can detect a significantly greater amount of parasites than the simplified iron hematoxylin method, especially with respect to Isospora belli, Cryptosporidium sp., Schistosoma mansoni, and Strongyloides stercoralis, which were not detected using hematoxylin. Endolimax nana and D. fragilis were detected more frequently on using hematoxylin, and the only parasite not found by the other methods was D. fragilis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Ritchie ENVELOPE(-128.387,-128.387,54.916,54.916) Revista da Sociedade Brasileira de Medicina Tropical 45 2 156 158 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
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English |
topic |
Dientamoeba fragilis Diarréia AIDS Hematoxilina férrica simplificada Arctic medicine. Tropical medicine RC955-962 |
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Dientamoeba fragilis Diarréia AIDS Hematoxilina férrica simplificada Arctic medicine. Tropical medicine RC955-962 Juliana Alves Garcia Sergio Cimerman Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique |
topic_facet |
Dientamoeba fragilis Diarréia AIDS Hematoxilina férrica simplificada Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Studies strongly indicate Dientamoeba fragilis as one of the causes of diarrhea in human immunodeficiency virus (HIV) patients. METHODS: The objective of the present study was to evaluate the prevalence of D. fragilis associated with the causes of diarrhea in 82 HIV/ AIDS patients hospitalized at the Instituto de Infectologia Emílio Ribas from September 2006 to November 2008. RESULTS: In total, 105 samples were collected from 82 patients. Unprotected sex was the most frequent cause of HIV infection (46.3%), followed by the use of injectable or non-injectable drugs (14.6%). Patients presented with viral loads of 49-750,000 copies/ mL (average: 73,849 ± 124,850 copies/mL) and CD4 counts ranging of 2-1,306 cells/mm³ (average: 159 ± 250 cells/mm³). On an average, the odds of obtaining a positive result by using the other techniques (Hoffman, Pons and Janer or Lutz; Ritchie) were 2.7 times higher than the chance of obtaining a positive result by using the simplified iron hematoxylin method. Significant differences were found between the methods (p = 0.003). CONCLUSIONS: The other techniques can detect a significantly greater amount of parasites than the simplified iron hematoxylin method, especially with respect to Isospora belli, Cryptosporidium sp., Schistosoma mansoni, and Strongyloides stercoralis, which were not detected using hematoxylin. Endolimax nana and D. fragilis were detected more frequently on using hematoxylin, and the only parasite not found by the other methods was D. fragilis. |
format |
Article in Journal/Newspaper |
author |
Juliana Alves Garcia Sergio Cimerman |
author_facet |
Juliana Alves Garcia Sergio Cimerman |
author_sort |
Juliana Alves Garcia |
title |
Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique |
title_short |
Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique |
title_full |
Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique |
title_fullStr |
Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique |
title_full_unstemmed |
Detection of Dientamoeba fragilis in patients with HIV/AIDS by using a simplified iron hematoxylin technique |
title_sort |
detection of dientamoeba fragilis in patients with hiv/aids by using a simplified iron hematoxylin technique |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2012 |
url |
https://doi.org/10.1590/S0037-86822012000200003 https://doaj.org/article/1db3f8371f5e440e897ee0d24ed357d1 |
long_lat |
ENVELOPE(-128.387,-128.387,54.916,54.916) |
geographic |
Arctic Ritchie |
geographic_facet |
Arctic Ritchie |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 45, Iss 2, Pp 156-158 (2012) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822012000200003&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822012000200003 https://doaj.org/article/1db3f8371f5e440e897ee0d24ed357d1 |
op_doi |
https://doi.org/10.1590/S0037-86822012000200003 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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45 |
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2 |
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156 |
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158 |
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