DNA methylation patterns provide insight into epigenetic regulation in the Pacific oyster ( Crassostrea gigas )
Abstract Background DNA methylation is an epigenetic mechanism with important regulatory functions in animals. While the mechanism itself is evolutionarily ancient, the distribution and function of DNA methylation is diverse both within and among phylogenetic groups. Although DNA methylation has bee...
Published in: | BMC Genomics |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2010
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1471-2164-11-483 https://doaj.org/article/1da9206fd4f944018ee7ae4866fff1a5 |
id |
ftdoajarticles:oai:doaj.org/article:1da9206fd4f944018ee7ae4866fff1a5 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:1da9206fd4f944018ee7ae4866fff1a5 2023-05-15T15:57:36+02:00 DNA methylation patterns provide insight into epigenetic regulation in the Pacific oyster ( Crassostrea gigas ) Gavery Mackenzie R Roberts Steven B 2010-08-01T00:00:00Z https://doi.org/10.1186/1471-2164-11-483 https://doaj.org/article/1da9206fd4f944018ee7ae4866fff1a5 EN eng BMC http://www.biomedcentral.com/1471-2164/11/483 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-11-483 1471-2164 https://doaj.org/article/1da9206fd4f944018ee7ae4866fff1a5 BMC Genomics, Vol 11, Iss 1, p 483 (2010) Biotechnology TP248.13-248.65 Genetics QH426-470 article 2010 ftdoajarticles https://doi.org/10.1186/1471-2164-11-483 2022-12-31T06:54:28Z Abstract Background DNA methylation is an epigenetic mechanism with important regulatory functions in animals. While the mechanism itself is evolutionarily ancient, the distribution and function of DNA methylation is diverse both within and among phylogenetic groups. Although DNA methylation has been well studied in mammals, there are limited data on invertebrates, particularly molluscs. Here we characterize the distribution and investigate potential functions of DNA methylation in the Pacific oyster ( Crassostrea gigas ). Results Methylation sensitive PCR and bisulfite sequencing PCR approaches were used to identify CpG methylation in C. gigas genes and demonstrated that this species possesses intragenic methylation. In silico analysis of CpGo/e ratios in publicly available sequence data suggests that DNA methylation is a common feature of the C. gigas genome, and that specific functional categories of genes have significantly different levels of methylation. Conclusions The Pacific oyster genome displays intragenic DNA methylation and contains genes necessary for DNA methylation in animals. Results of this investigation suggest that DNA methylation has regulatory functions in Crassostrea gigas , particularly in gene families that have inducible expression, including those involved in stress and environmental responses. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific BMC Genomics 11 1 483 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Biotechnology TP248.13-248.65 Genetics QH426-470 |
spellingShingle |
Biotechnology TP248.13-248.65 Genetics QH426-470 Gavery Mackenzie R Roberts Steven B DNA methylation patterns provide insight into epigenetic regulation in the Pacific oyster ( Crassostrea gigas ) |
topic_facet |
Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background DNA methylation is an epigenetic mechanism with important regulatory functions in animals. While the mechanism itself is evolutionarily ancient, the distribution and function of DNA methylation is diverse both within and among phylogenetic groups. Although DNA methylation has been well studied in mammals, there are limited data on invertebrates, particularly molluscs. Here we characterize the distribution and investigate potential functions of DNA methylation in the Pacific oyster ( Crassostrea gigas ). Results Methylation sensitive PCR and bisulfite sequencing PCR approaches were used to identify CpG methylation in C. gigas genes and demonstrated that this species possesses intragenic methylation. In silico analysis of CpGo/e ratios in publicly available sequence data suggests that DNA methylation is a common feature of the C. gigas genome, and that specific functional categories of genes have significantly different levels of methylation. Conclusions The Pacific oyster genome displays intragenic DNA methylation and contains genes necessary for DNA methylation in animals. Results of this investigation suggest that DNA methylation has regulatory functions in Crassostrea gigas , particularly in gene families that have inducible expression, including those involved in stress and environmental responses. |
format |
Article in Journal/Newspaper |
author |
Gavery Mackenzie R Roberts Steven B |
author_facet |
Gavery Mackenzie R Roberts Steven B |
author_sort |
Gavery Mackenzie R |
title |
DNA methylation patterns provide insight into epigenetic regulation in the Pacific oyster ( Crassostrea gigas ) |
title_short |
DNA methylation patterns provide insight into epigenetic regulation in the Pacific oyster ( Crassostrea gigas ) |
title_full |
DNA methylation patterns provide insight into epigenetic regulation in the Pacific oyster ( Crassostrea gigas ) |
title_fullStr |
DNA methylation patterns provide insight into epigenetic regulation in the Pacific oyster ( Crassostrea gigas ) |
title_full_unstemmed |
DNA methylation patterns provide insight into epigenetic regulation in the Pacific oyster ( Crassostrea gigas ) |
title_sort |
dna methylation patterns provide insight into epigenetic regulation in the pacific oyster ( crassostrea gigas ) |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1471-2164-11-483 https://doaj.org/article/1da9206fd4f944018ee7ae4866fff1a5 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
BMC Genomics, Vol 11, Iss 1, p 483 (2010) |
op_relation |
http://www.biomedcentral.com/1471-2164/11/483 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-11-483 1471-2164 https://doaj.org/article/1da9206fd4f944018ee7ae4866fff1a5 |
op_doi |
https://doi.org/10.1186/1471-2164-11-483 |
container_title |
BMC Genomics |
container_volume |
11 |
container_issue |
1 |
container_start_page |
483 |
_version_ |
1766393281687584768 |