Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus
In this study, we describe the fate of fatty acids that are incorporated from the lumen by the posterior midgut epithelium of Rhodnius prolixus and the biosynthesis of lipids. We also demonstrate that neutral lipids (NL) are transferred to the haemolymphatic lipophorin (Lp) and that phospholipids re...
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Instituto Oswaldo Cruz, Ministério da Saúde
2013
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ftdoajarticles:oai:doaj.org/article:1d72c6d639214a1e9b20bd571775ee0f 2023-05-15T15:07:04+02:00 Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus Paula Rego Bittencourt-Cunha Livia Silva-Cardoso Giselle Almeida de Oliveira Jose Roberto da Silva Alan Barbosa da Silveira George Eduardo Gabriel Kluck Michele Souza-Lima Katia Calp Gondim Marilvia Dansa-Petretsky Carlos Peres Silva Hatisaburo Masuda Mario Alberto Cardoso da Silva Neto Georgia Correa Atella 2013-06-01T00:00:00Z https://doi.org/10.1590/S0074-0276108042013016 https://doaj.org/article/1d72c6d639214a1e9b20bd571775ee0f EN eng Instituto Oswaldo Cruz, Ministério da Saúde http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000400494&lng=en&tlng=en https://doaj.org/toc/1678-8060 1678-8060 doi:10.1590/S0074-0276108042013016 https://doaj.org/article/1d72c6d639214a1e9b20bd571775ee0f Memórias do Instituto Oswaldo Cruz., Vol 108, Iss 4, Pp 494-500 (2013) lipid metabolism lipoprotein lipid transport phospholipids midgut Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 article 2013 ftdoajarticles https://doi.org/10.1590/S0074-0276108042013016 2023-01-08T01:37:22Z In this study, we describe the fate of fatty acids that are incorporated from the lumen by the posterior midgut epithelium of Rhodnius prolixus and the biosynthesis of lipids. We also demonstrate that neutral lipids (NL) are transferred to the haemolymphatic lipophorin (Lp) and that phospholipids remain in the tissue in which they are organised into perimicrovillar membranes (PMMs). 3H-palmitic acid added at the luminal side of isolated midguts of R. prolixus females was readily absorbed and was used to synthesise phospholipids (80%) and NL (20%). The highest incorporation of 3H-palmitic acid was on the first day after a blood meal. The amounts of diacylglycerol (DG) and triacylglycerol synthesised by the tissue decreased in the presence of Lp in the incubation medium. The metabolic fates of 3H-lipids synthesised by the posterior midgut were followed and it was observed that DG was the major lipid released to Lp particles. However, the majority of phospholipids were not transferred to Lp, but remained in the tissue. The phospholipids that were synthesised and accumulated in the posterior midgut were found to be associated with Rhodnius luminal contents as structural components of PMMs. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Memórias do Instituto Oswaldo Cruz 108 4 494 500 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
lipid metabolism lipoprotein lipid transport phospholipids midgut Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
spellingShingle |
lipid metabolism lipoprotein lipid transport phospholipids midgut Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 Paula Rego Bittencourt-Cunha Livia Silva-Cardoso Giselle Almeida de Oliveira Jose Roberto da Silva Alan Barbosa da Silveira George Eduardo Gabriel Kluck Michele Souza-Lima Katia Calp Gondim Marilvia Dansa-Petretsky Carlos Peres Silva Hatisaburo Masuda Mario Alberto Cardoso da Silva Neto Georgia Correa Atella Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus |
topic_facet |
lipid metabolism lipoprotein lipid transport phospholipids midgut Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
description |
In this study, we describe the fate of fatty acids that are incorporated from the lumen by the posterior midgut epithelium of Rhodnius prolixus and the biosynthesis of lipids. We also demonstrate that neutral lipids (NL) are transferred to the haemolymphatic lipophorin (Lp) and that phospholipids remain in the tissue in which they are organised into perimicrovillar membranes (PMMs). 3H-palmitic acid added at the luminal side of isolated midguts of R. prolixus females was readily absorbed and was used to synthesise phospholipids (80%) and NL (20%). The highest incorporation of 3H-palmitic acid was on the first day after a blood meal. The amounts of diacylglycerol (DG) and triacylglycerol synthesised by the tissue decreased in the presence of Lp in the incubation medium. The metabolic fates of 3H-lipids synthesised by the posterior midgut were followed and it was observed that DG was the major lipid released to Lp particles. However, the majority of phospholipids were not transferred to Lp, but remained in the tissue. The phospholipids that were synthesised and accumulated in the posterior midgut were found to be associated with Rhodnius luminal contents as structural components of PMMs. |
format |
Article in Journal/Newspaper |
author |
Paula Rego Bittencourt-Cunha Livia Silva-Cardoso Giselle Almeida de Oliveira Jose Roberto da Silva Alan Barbosa da Silveira George Eduardo Gabriel Kluck Michele Souza-Lima Katia Calp Gondim Marilvia Dansa-Petretsky Carlos Peres Silva Hatisaburo Masuda Mario Alberto Cardoso da Silva Neto Georgia Correa Atella |
author_facet |
Paula Rego Bittencourt-Cunha Livia Silva-Cardoso Giselle Almeida de Oliveira Jose Roberto da Silva Alan Barbosa da Silveira George Eduardo Gabriel Kluck Michele Souza-Lima Katia Calp Gondim Marilvia Dansa-Petretsky Carlos Peres Silva Hatisaburo Masuda Mario Alberto Cardoso da Silva Neto Georgia Correa Atella |
author_sort |
Paula Rego Bittencourt-Cunha |
title |
Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus |
title_short |
Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus |
title_full |
Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus |
title_fullStr |
Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus |
title_full_unstemmed |
Perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of Rhodnius prolixus |
title_sort |
perimicrovillar membrane assembly: the fate of phospholipids synthesised by the midgut of rhodnius prolixus |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2013 |
url |
https://doi.org/10.1590/S0074-0276108042013016 https://doaj.org/article/1d72c6d639214a1e9b20bd571775ee0f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Memórias do Instituto Oswaldo Cruz., Vol 108, Iss 4, Pp 494-500 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000400494&lng=en&tlng=en https://doaj.org/toc/1678-8060 1678-8060 doi:10.1590/S0074-0276108042013016 https://doaj.org/article/1d72c6d639214a1e9b20bd571775ee0f |
op_doi |
https://doi.org/10.1590/S0074-0276108042013016 |
container_title |
Memórias do Instituto Oswaldo Cruz |
container_volume |
108 |
container_issue |
4 |
container_start_page |
494 |
op_container_end_page |
500 |
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1766338640398516224 |