Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers
The apple fruit moth Argyresthia conjugella (Lepidoptera, Yponomeutidae) is a seed predator of rowan (Sorbus aucuparia) and is distributed in Europe and Asia. In Fennoscandia (Finland, Norway and Sweden), rowan fruit production is low every 2–4 years, and apple (Malus domestica) functions as an alte...
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ftdoajarticles:oai:doaj.org/article:1d53d1b506db4b38babddff90f6fbf77 2023-05-15T16:11:59+02:00 Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers Abdelhameed Elameen Hans Geir Eiken Ida Fløystad Geir Knudsen Snorre B. Hagen 2018-04-01T00:00:00Z https://doi.org/10.3390/molecules23040850 https://doaj.org/article/1d53d1b506db4b38babddff90f6fbf77 EN eng MDPI AG http://www.mdpi.com/1420-3049/23/4/850 https://doaj.org/toc/1420-3049 1420-3049 doi:10.3390/molecules23040850 https://doaj.org/article/1d53d1b506db4b38babddff90f6fbf77 Molecules, Vol 23, Iss 4, p 850 (2018) Argyresthia conjugella Lepidoptera multiplex PCR next-generation sequencing (NGS) tetranucleotide repeats Organic chemistry QD241-441 article 2018 ftdoajarticles https://doi.org/10.3390/molecules23040850 2022-12-31T12:50:36Z The apple fruit moth Argyresthia conjugella (Lepidoptera, Yponomeutidae) is a seed predator of rowan (Sorbus aucuparia) and is distributed in Europe and Asia. In Fennoscandia (Finland, Norway and Sweden), rowan fruit production is low every 2–4 years, and apple (Malus domestica) functions as an alternative host, resulting in economic loss in apple crops in inter-mast years. We have used Illumina MiSeq sequencing to identify a set of 19 novel tetra-nucleotide short tandem repeats (STRs) in Argyresthia conjugella. Such motifs are recommended for genetic monitoring, which may help to determine the eco-evolutionary processes acting on this pest insect. The 19 STRs were optimized and amplified into five multiplex PCR reactions. We tested individuals collected from Norway and Sweden (n = 64), and detected very high genetic variation (average 13.6 alleles, He = 0.75) compared to most other Lepidoptera species studied so far. Spatial genetic differentiation was low and gene flow was high in the test populations, although two non-spatial clusters could be detected. We conclude that this set of genetic markers may be a useful resource for population genetic monitoring of this economical important insect species. Article in Journal/Newspaper Fennoscandia Directory of Open Access Journals: DOAJ Articles Norway Malus ENVELOPE(-65.734,-65.734,-66.229,-66.229) Molecules 23 4 850 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Argyresthia conjugella Lepidoptera multiplex PCR next-generation sequencing (NGS) tetranucleotide repeats Organic chemistry QD241-441 |
spellingShingle |
Argyresthia conjugella Lepidoptera multiplex PCR next-generation sequencing (NGS) tetranucleotide repeats Organic chemistry QD241-441 Abdelhameed Elameen Hans Geir Eiken Ida Fløystad Geir Knudsen Snorre B. Hagen Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers |
topic_facet |
Argyresthia conjugella Lepidoptera multiplex PCR next-generation sequencing (NGS) tetranucleotide repeats Organic chemistry QD241-441 |
description |
The apple fruit moth Argyresthia conjugella (Lepidoptera, Yponomeutidae) is a seed predator of rowan (Sorbus aucuparia) and is distributed in Europe and Asia. In Fennoscandia (Finland, Norway and Sweden), rowan fruit production is low every 2–4 years, and apple (Malus domestica) functions as an alternative host, resulting in economic loss in apple crops in inter-mast years. We have used Illumina MiSeq sequencing to identify a set of 19 novel tetra-nucleotide short tandem repeats (STRs) in Argyresthia conjugella. Such motifs are recommended for genetic monitoring, which may help to determine the eco-evolutionary processes acting on this pest insect. The 19 STRs were optimized and amplified into five multiplex PCR reactions. We tested individuals collected from Norway and Sweden (n = 64), and detected very high genetic variation (average 13.6 alleles, He = 0.75) compared to most other Lepidoptera species studied so far. Spatial genetic differentiation was low and gene flow was high in the test populations, although two non-spatial clusters could be detected. We conclude that this set of genetic markers may be a useful resource for population genetic monitoring of this economical important insect species. |
format |
Article in Journal/Newspaper |
author |
Abdelhameed Elameen Hans Geir Eiken Ida Fløystad Geir Knudsen Snorre B. Hagen |
author_facet |
Abdelhameed Elameen Hans Geir Eiken Ida Fløystad Geir Knudsen Snorre B. Hagen |
author_sort |
Abdelhameed Elameen |
title |
Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers |
title_short |
Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers |
title_full |
Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers |
title_fullStr |
Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers |
title_full_unstemmed |
Monitoring of the Apple Fruit Moth: Detection of Genetic Variation and Structure Applying a Novel Multiplex Set of 19 STR Markers |
title_sort |
monitoring of the apple fruit moth: detection of genetic variation and structure applying a novel multiplex set of 19 str markers |
publisher |
MDPI AG |
publishDate |
2018 |
url |
https://doi.org/10.3390/molecules23040850 https://doaj.org/article/1d53d1b506db4b38babddff90f6fbf77 |
long_lat |
ENVELOPE(-65.734,-65.734,-66.229,-66.229) |
geographic |
Norway Malus |
geographic_facet |
Norway Malus |
genre |
Fennoscandia |
genre_facet |
Fennoscandia |
op_source |
Molecules, Vol 23, Iss 4, p 850 (2018) |
op_relation |
http://www.mdpi.com/1420-3049/23/4/850 https://doaj.org/toc/1420-3049 1420-3049 doi:10.3390/molecules23040850 https://doaj.org/article/1d53d1b506db4b38babddff90f6fbf77 |
op_doi |
https://doi.org/10.3390/molecules23040850 |
container_title |
Molecules |
container_volume |
23 |
container_issue |
4 |
container_start_page |
850 |
_version_ |
1765997200692740096 |