The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster
Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein (CgIFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cy...
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ftdoajarticles:oai:doaj.org/article:1d270f6de69a4d09a3c53192d5d77e43 2023-05-15T15:58:12+02:00 The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster Xue Qiao Yanan Zong Zhaoqun Liu Zhaojun Wu Yuanmei Li Lingling Wang Linsheng Song 2021-06-01T00:00:00Z https://doi.org/10.3389/fimmu.2021.689783 https://doaj.org/article/1d270f6de69a4d09a3c53192d5d77e43 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fimmu.2021.689783/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2021.689783 https://doaj.org/article/1d270f6de69a4d09a3c53192d5d77e43 Frontiers in Immunology, Vol 12 (2021) Crassostrea gigas interferon-like system cyclic GMP-AMP (cGAMP) synthase (cGAS) stimulator of interferon genes (STING) antiviral immunity Immunologic diseases. Allergy RC581-607 article 2021 ftdoajarticles https://doi.org/10.3389/fimmu.2021.689783 2022-12-31T05:09:54Z Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein (CgIFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cytokines. In the present study, the CgIFNLP protein in hemocytes was observed to increase after Poly (I:C) stimulation. After CgIFNLP was knocked down by RNAi, the mRNA expression of IFN-stimulated genes (CgISGs) was significantly inhibited. Both cyclic GMP-AMP synthase (CgcGAS) and stimulator of interferon gene (CgSTING) identified from oyster were able to recognize the double-stranded nucleic acid [Poly (I:C) and dsDNA] and expressed at high level after Poly (I:C) stimulation. The expression of CgIFNLP and interferon regulatory factors (CgIRF1/8) and the nuclear translocation of CgIRF8 were all suppressed in CgcGAS-RNAi or CgSTING-RNAi oysters after Poly (I:C) stimulation. The expression level of CgSTING and TANK binding kinase1 (CgTBK1) did not decrease in CgcGAS-RNAi oysters. After CgSTING was knocked down, the high expression of CgTBK1 induced by Poly (I:C) was prevented significantly. These results indicated that there was a primitive IFN-like antiviral mechanism dependent on the cGAS/STING–TBK1–IRFs regulatory axis in mollusks, which was different from the classic cGAS–STING–TBK1 signal pathway in mammals. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Frontiers in Immunology 12 |
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language |
English |
topic |
Crassostrea gigas interferon-like system cyclic GMP-AMP (cGAMP) synthase (cGAS) stimulator of interferon genes (STING) antiviral immunity Immunologic diseases. Allergy RC581-607 |
spellingShingle |
Crassostrea gigas interferon-like system cyclic GMP-AMP (cGAMP) synthase (cGAS) stimulator of interferon genes (STING) antiviral immunity Immunologic diseases. Allergy RC581-607 Xue Qiao Yanan Zong Zhaoqun Liu Zhaojun Wu Yuanmei Li Lingling Wang Linsheng Song The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster |
topic_facet |
Crassostrea gigas interferon-like system cyclic GMP-AMP (cGAMP) synthase (cGAS) stimulator of interferon genes (STING) antiviral immunity Immunologic diseases. Allergy RC581-607 |
description |
Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein (CgIFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cytokines. In the present study, the CgIFNLP protein in hemocytes was observed to increase after Poly (I:C) stimulation. After CgIFNLP was knocked down by RNAi, the mRNA expression of IFN-stimulated genes (CgISGs) was significantly inhibited. Both cyclic GMP-AMP synthase (CgcGAS) and stimulator of interferon gene (CgSTING) identified from oyster were able to recognize the double-stranded nucleic acid [Poly (I:C) and dsDNA] and expressed at high level after Poly (I:C) stimulation. The expression of CgIFNLP and interferon regulatory factors (CgIRF1/8) and the nuclear translocation of CgIRF8 were all suppressed in CgcGAS-RNAi or CgSTING-RNAi oysters after Poly (I:C) stimulation. The expression level of CgSTING and TANK binding kinase1 (CgTBK1) did not decrease in CgcGAS-RNAi oysters. After CgSTING was knocked down, the high expression of CgTBK1 induced by Poly (I:C) was prevented significantly. These results indicated that there was a primitive IFN-like antiviral mechanism dependent on the cGAS/STING–TBK1–IRFs regulatory axis in mollusks, which was different from the classic cGAS–STING–TBK1 signal pathway in mammals. |
format |
Article in Journal/Newspaper |
author |
Xue Qiao Yanan Zong Zhaoqun Liu Zhaojun Wu Yuanmei Li Lingling Wang Linsheng Song |
author_facet |
Xue Qiao Yanan Zong Zhaoqun Liu Zhaojun Wu Yuanmei Li Lingling Wang Linsheng Song |
author_sort |
Xue Qiao |
title |
The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster |
title_short |
The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster |
title_full |
The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster |
title_fullStr |
The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster |
title_full_unstemmed |
The cGAS/STING–TBK1–IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster |
title_sort |
cgas/sting–tbk1–irf regulatory axis orchestrates a primitive interferon-like antiviral mechanism in oyster |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fimmu.2021.689783 https://doaj.org/article/1d270f6de69a4d09a3c53192d5d77e43 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Frontiers in Immunology, Vol 12 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fimmu.2021.689783/full https://doaj.org/toc/1664-3224 1664-3224 doi:10.3389/fimmu.2021.689783 https://doaj.org/article/1d270f6de69a4d09a3c53192d5d77e43 |
op_doi |
https://doi.org/10.3389/fimmu.2021.689783 |
container_title |
Frontiers in Immunology |
container_volume |
12 |
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1766393930439458816 |