Annona muricata fruit extract protects against diethylnitrosamine-induced hepatocellular cancer in rats
Objective: To evaluate the anticancer potentials of Annona muricata fruit by in vitro and in vivo methods. Methods: The ethanolic extract of Annona muricata fruit was prepared by Soxhlet extraction method and further fractionated with petroleum ether, ethyl acetate and chloroform. The fractions were...
Published in: | Asian Pacific Journal of Tropical Medicine |
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Wolters Kluwer Medknow Publications
2019
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ftdoajarticles:oai:doaj.org/article:1ced966f5c0a4969a4c6973a39b8b124 2023-05-15T15:16:45+02:00 Annona muricata fruit extract protects against diethylnitrosamine-induced hepatocellular cancer in rats Subin Varghese Thomas John Paul Akhila Hosur Shrungeswara Subhankar Biswas Aarti Abhishek Shah Chandrashekar Kodangala Subraya Yogendra Nayak 2019-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.261274 https://doaj.org/article/1ced966f5c0a4969a4c6973a39b8b124 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=6;spage=272;epage=282;aulast=Thomas https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.261274 https://doaj.org/article/1ced966f5c0a4969a4c6973a39b8b124 Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 6, Pp 272-282 (2019) hepg2-cells apoptosis liver cancer acetogenins rat liver cancer scratch wound assay Arctic medicine. Tropical medicine RC955-962 article 2019 ftdoajarticles https://doi.org/10.4103/1995-7645.261274 2022-12-31T04:38:01Z Objective: To evaluate the anticancer potentials of Annona muricata fruit by in vitro and in vivo methods. Methods: The ethanolic extract of Annona muricata fruit was prepared by Soxhlet extraction method and further fractionated with petroleum ether, ethyl acetate and chloroform. The fractions were tested for cytotoxicity, apoptosis, scratch wound assay, and cell cycle analysis. IC50, apoptotic index and percentage cell migration were determined using HepG2 cells. For the in vivo studies, hepatocellular carcinoma was induced by administering 0.01% diethylnitrosamine (DEN) in drinking water in Wistar rats. In pre-treatment, rats were co-administered 200 mg/kg of fruit extract with DEN for 14 weeks. In post-treatment, the extract was co-administered after 8-weeks of DEN-induction for 14 weeks. Liver function test, haematological test, oxidative stress markers, relative liver weight, number of cancer nodules and histopathological parameters were determined. Results: Annona muricata fruit extract =significantly lowered cell proliferation counts. The chloroform-fraction possessed higher activity [IC50=(53.7±4.3) μg/mL]. The chloroform fraction inhibited cell migration, which was significant compared to curcumin. Further investigations regarding the mode of anticancer activity revealed that the chloroform fraction induced apoptosis. The cell cycle analysis indicated that cells were being arrested at G0/G1. In the in vivo studies, the DEN-control group showed a significant decrease in body weights with increased mortality rate, hepatic nodules, and impairment of liver function compared to normal rats. The rats pre-treated and post-treated with the extract showed positive results with significant improvement in the parameters that were adversely affected by DEN. In addition, other adverse effects of DEN, such as blood dyscrasias and hepatic endogenous antioxidant, were significantly attenuated by Annona muricata fruit extract. Conclusions: The Annona muricata fruit extract has anticancer activity when tested by in ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 12 6 272 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
hepg2-cells apoptosis liver cancer acetogenins rat liver cancer scratch wound assay Arctic medicine. Tropical medicine RC955-962 |
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hepg2-cells apoptosis liver cancer acetogenins rat liver cancer scratch wound assay Arctic medicine. Tropical medicine RC955-962 Subin Varghese Thomas John Paul Akhila Hosur Shrungeswara Subhankar Biswas Aarti Abhishek Shah Chandrashekar Kodangala Subraya Yogendra Nayak Annona muricata fruit extract protects against diethylnitrosamine-induced hepatocellular cancer in rats |
topic_facet |
hepg2-cells apoptosis liver cancer acetogenins rat liver cancer scratch wound assay Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To evaluate the anticancer potentials of Annona muricata fruit by in vitro and in vivo methods. Methods: The ethanolic extract of Annona muricata fruit was prepared by Soxhlet extraction method and further fractionated with petroleum ether, ethyl acetate and chloroform. The fractions were tested for cytotoxicity, apoptosis, scratch wound assay, and cell cycle analysis. IC50, apoptotic index and percentage cell migration were determined using HepG2 cells. For the in vivo studies, hepatocellular carcinoma was induced by administering 0.01% diethylnitrosamine (DEN) in drinking water in Wistar rats. In pre-treatment, rats were co-administered 200 mg/kg of fruit extract with DEN for 14 weeks. In post-treatment, the extract was co-administered after 8-weeks of DEN-induction for 14 weeks. Liver function test, haematological test, oxidative stress markers, relative liver weight, number of cancer nodules and histopathological parameters were determined. Results: Annona muricata fruit extract =significantly lowered cell proliferation counts. The chloroform-fraction possessed higher activity [IC50=(53.7±4.3) μg/mL]. The chloroform fraction inhibited cell migration, which was significant compared to curcumin. Further investigations regarding the mode of anticancer activity revealed that the chloroform fraction induced apoptosis. The cell cycle analysis indicated that cells were being arrested at G0/G1. In the in vivo studies, the DEN-control group showed a significant decrease in body weights with increased mortality rate, hepatic nodules, and impairment of liver function compared to normal rats. The rats pre-treated and post-treated with the extract showed positive results with significant improvement in the parameters that were adversely affected by DEN. In addition, other adverse effects of DEN, such as blood dyscrasias and hepatic endogenous antioxidant, were significantly attenuated by Annona muricata fruit extract. Conclusions: The Annona muricata fruit extract has anticancer activity when tested by in ... |
format |
Article in Journal/Newspaper |
author |
Subin Varghese Thomas John Paul Akhila Hosur Shrungeswara Subhankar Biswas Aarti Abhishek Shah Chandrashekar Kodangala Subraya Yogendra Nayak |
author_facet |
Subin Varghese Thomas John Paul Akhila Hosur Shrungeswara Subhankar Biswas Aarti Abhishek Shah Chandrashekar Kodangala Subraya Yogendra Nayak |
author_sort |
Subin Varghese Thomas |
title |
Annona muricata fruit extract protects against diethylnitrosamine-induced hepatocellular cancer in rats |
title_short |
Annona muricata fruit extract protects against diethylnitrosamine-induced hepatocellular cancer in rats |
title_full |
Annona muricata fruit extract protects against diethylnitrosamine-induced hepatocellular cancer in rats |
title_fullStr |
Annona muricata fruit extract protects against diethylnitrosamine-induced hepatocellular cancer in rats |
title_full_unstemmed |
Annona muricata fruit extract protects against diethylnitrosamine-induced hepatocellular cancer in rats |
title_sort |
annona muricata fruit extract protects against diethylnitrosamine-induced hepatocellular cancer in rats |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2019 |
url |
https://doi.org/10.4103/1995-7645.261274 https://doaj.org/article/1ced966f5c0a4969a4c6973a39b8b124 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 12, Iss 6, Pp 272-282 (2019) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2019;volume=12;issue=6;spage=272;epage=282;aulast=Thomas https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.261274 https://doaj.org/article/1ced966f5c0a4969a4c6973a39b8b124 |
op_doi |
https://doi.org/10.4103/1995-7645.261274 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
12 |
container_issue |
6 |
container_start_page |
272 |
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1766347041830600704 |