Effective chikungunya virus-like particle vaccine produced in insect cells.
The emerging arthritogenic, mosquito-borne chikungunya virus (CHIKV) causes severe disease in humans and represents a serious public health threat in countries where Aedes spp mosquitoes are present. This study describes for the first time the successful production of CHIKV virus-like particles (VLP...
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ftdoajarticles:oai:doaj.org/article:1cb27c031e31450cabba3c0ff80f2227 2023-05-15T15:04:15+02:00 Effective chikungunya virus-like particle vaccine produced in insect cells. Stefan W Metz Joy Gardner Corinne Geertsema Thuy T Le Lucas Goh Just M Vlak Andreas Suhrbier Gorben P Pijlman 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002124 https://doaj.org/article/1cb27c031e31450cabba3c0ff80f2227 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3597470?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 doi:10.1371/journal.pntd.0002124 1935-2727 1935-2735 https://doaj.org/article/1cb27c031e31450cabba3c0ff80f2227 PLoS Neglected Tropical Diseases, Vol 7, Iss 3, p e2124 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002124 2022-12-31T14:06:51Z The emerging arthritogenic, mosquito-borne chikungunya virus (CHIKV) causes severe disease in humans and represents a serious public health threat in countries where Aedes spp mosquitoes are present. This study describes for the first time the successful production of CHIKV virus-like particles (VLPs) in insect cells using recombinant baculoviruses. This well-established expression system is rapidly scalable to volumes required for epidemic responses and proved well suited for processing of CHIKV glycoproteins and production of enveloped VLPs. Herein we show that a single immunization with 1 µg of non-adjuvanted CHIKV VLPs induced high titer neutralizing antibody responses and provided complete protection against viraemia and joint inflammation upon challenge with the Réunion Island CHIKV strain in an adult wild-type mouse model of CHIKV disease. CHIKV VLPs produced in insect cells using recombinant baculoviruses thus represents as a new, safe, non-replicating and effective vaccine candidate against CHIKV infections. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 3 e2124 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Stefan W Metz Joy Gardner Corinne Geertsema Thuy T Le Lucas Goh Just M Vlak Andreas Suhrbier Gorben P Pijlman Effective chikungunya virus-like particle vaccine produced in insect cells. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The emerging arthritogenic, mosquito-borne chikungunya virus (CHIKV) causes severe disease in humans and represents a serious public health threat in countries where Aedes spp mosquitoes are present. This study describes for the first time the successful production of CHIKV virus-like particles (VLPs) in insect cells using recombinant baculoviruses. This well-established expression system is rapidly scalable to volumes required for epidemic responses and proved well suited for processing of CHIKV glycoproteins and production of enveloped VLPs. Herein we show that a single immunization with 1 µg of non-adjuvanted CHIKV VLPs induced high titer neutralizing antibody responses and provided complete protection against viraemia and joint inflammation upon challenge with the Réunion Island CHIKV strain in an adult wild-type mouse model of CHIKV disease. CHIKV VLPs produced in insect cells using recombinant baculoviruses thus represents as a new, safe, non-replicating and effective vaccine candidate against CHIKV infections. |
format |
Article in Journal/Newspaper |
author |
Stefan W Metz Joy Gardner Corinne Geertsema Thuy T Le Lucas Goh Just M Vlak Andreas Suhrbier Gorben P Pijlman |
author_facet |
Stefan W Metz Joy Gardner Corinne Geertsema Thuy T Le Lucas Goh Just M Vlak Andreas Suhrbier Gorben P Pijlman |
author_sort |
Stefan W Metz |
title |
Effective chikungunya virus-like particle vaccine produced in insect cells. |
title_short |
Effective chikungunya virus-like particle vaccine produced in insect cells. |
title_full |
Effective chikungunya virus-like particle vaccine produced in insect cells. |
title_fullStr |
Effective chikungunya virus-like particle vaccine produced in insect cells. |
title_full_unstemmed |
Effective chikungunya virus-like particle vaccine produced in insect cells. |
title_sort |
effective chikungunya virus-like particle vaccine produced in insect cells. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002124 https://doaj.org/article/1cb27c031e31450cabba3c0ff80f2227 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 3, p e2124 (2013) |
op_relation |
http://europepmc.org/articles/PMC3597470?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 doi:10.1371/journal.pntd.0002124 1935-2727 1935-2735 https://doaj.org/article/1cb27c031e31450cabba3c0ff80f2227 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002124 |
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PLoS Neglected Tropical Diseases |
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7 |
container_issue |
3 |
container_start_page |
e2124 |
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1766336050967347200 |