Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures
Abstract Background In order to understand the role of herbivores in trophic webs, it is essential to know what they feed on. Diet analysis is, however, a challenge in many small herbivores with a secretive life style. In this paper, we compare novel (high-throughput pyrosequencing) DNA barcoding te...
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ftdoajarticles:oai:doaj.org/article:1ca37a08b5404ee89e0f131a60d63436 2023-05-15T18:28:24+02:00 Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures Sønstebø Jørn H Brysting Anne K Brochmann Christian Gielly Ludovic Miquel Christian Coissac Eric Valentini Alice Soininen Eeva M Ims Rolf A Yoccoz Nigel G Taberlet Pierre 2009-08-01T00:00:00Z https://doi.org/10.1186/1742-9994-6-16 https://doaj.org/article/1ca37a08b5404ee89e0f131a60d63436 EN eng BMC http://www.frontiersinzoology.com/content/6/1/16 https://doaj.org/toc/1742-9994 doi:10.1186/1742-9994-6-16 1742-9994 https://doaj.org/article/1ca37a08b5404ee89e0f131a60d63436 Frontiers in Zoology, Vol 6, Iss 1, p 16 (2009) Zoology QL1-991 article 2009 ftdoajarticles https://doi.org/10.1186/1742-9994-6-16 2022-12-31T09:10:27Z Abstract Background In order to understand the role of herbivores in trophic webs, it is essential to know what they feed on. Diet analysis is, however, a challenge in many small herbivores with a secretive life style. In this paper, we compare novel (high-throughput pyrosequencing) DNA barcoding technology for plant mixture with traditional microhistological method. We analysed stomach contents of two ecologically important subarctic vole species, Microtus oeconomus and Myodes rufocanus , with the two methods. DNA barcoding was conducted using the P6-loop of the chloroplast trn L (UAA) intron. Results Although the identified plant taxa in the diets matched relatively well between the two methods, DNA barcoding gave by far taxonomically more detailed results. Quantitative comparison of results was difficult, mainly due to low taxonomic resolution of the microhistological method, which also in part explained discrepancies between the methods. Other discrepancies were likely due to biases mostly in the microhistological analysis. Conclusion We conclude that DNA barcoding opens up for new possibilities in the study of plant-herbivore interactions, giving a detailed and relatively unbiased picture of food utilization of herbivores. Article in Journal/Newspaper Subarctic Directory of Open Access Journals: DOAJ Articles Frontiers in Zoology 6 1 16 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Zoology QL1-991 |
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Zoology QL1-991 Sønstebø Jørn H Brysting Anne K Brochmann Christian Gielly Ludovic Miquel Christian Coissac Eric Valentini Alice Soininen Eeva M Ims Rolf A Yoccoz Nigel G Taberlet Pierre Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
topic_facet |
Zoology QL1-991 |
description |
Abstract Background In order to understand the role of herbivores in trophic webs, it is essential to know what they feed on. Diet analysis is, however, a challenge in many small herbivores with a secretive life style. In this paper, we compare novel (high-throughput pyrosequencing) DNA barcoding technology for plant mixture with traditional microhistological method. We analysed stomach contents of two ecologically important subarctic vole species, Microtus oeconomus and Myodes rufocanus , with the two methods. DNA barcoding was conducted using the P6-loop of the chloroplast trn L (UAA) intron. Results Although the identified plant taxa in the diets matched relatively well between the two methods, DNA barcoding gave by far taxonomically more detailed results. Quantitative comparison of results was difficult, mainly due to low taxonomic resolution of the microhistological method, which also in part explained discrepancies between the methods. Other discrepancies were likely due to biases mostly in the microhistological analysis. Conclusion We conclude that DNA barcoding opens up for new possibilities in the study of plant-herbivore interactions, giving a detailed and relatively unbiased picture of food utilization of herbivores. |
format |
Article in Journal/Newspaper |
author |
Sønstebø Jørn H Brysting Anne K Brochmann Christian Gielly Ludovic Miquel Christian Coissac Eric Valentini Alice Soininen Eeva M Ims Rolf A Yoccoz Nigel G Taberlet Pierre |
author_facet |
Sønstebø Jørn H Brysting Anne K Brochmann Christian Gielly Ludovic Miquel Christian Coissac Eric Valentini Alice Soininen Eeva M Ims Rolf A Yoccoz Nigel G Taberlet Pierre |
author_sort |
Sønstebø Jørn H |
title |
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_short |
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_full |
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_fullStr |
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_full_unstemmed |
Analysing diet of small herbivores: the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
title_sort |
analysing diet of small herbivores: the efficiency of dna barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixtures |
publisher |
BMC |
publishDate |
2009 |
url |
https://doi.org/10.1186/1742-9994-6-16 https://doaj.org/article/1ca37a08b5404ee89e0f131a60d63436 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Frontiers in Zoology, Vol 6, Iss 1, p 16 (2009) |
op_relation |
http://www.frontiersinzoology.com/content/6/1/16 https://doaj.org/toc/1742-9994 doi:10.1186/1742-9994-6-16 1742-9994 https://doaj.org/article/1ca37a08b5404ee89e0f131a60d63436 |
op_doi |
https://doi.org/10.1186/1742-9994-6-16 |
container_title |
Frontiers in Zoology |
container_volume |
6 |
container_issue |
1 |
container_start_page |
16 |
_version_ |
1766210878216077312 |