Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack ( Theragra chalcogramma ) Skin
Collagen hydrolysate were extracted from Alaska pollock skin using enzymatic (EAE), ultrasound (UAE), or combination of enzymatic and ultrasound (EAE+UAE) treatment. Control (C) was not treated with enzymatic or ultrasound. The extracts from C, EAE, UAE, and EAE+UAE were fractionated with ≤3, 3–10,...
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2022
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Online Access: | https://doi.org/10.3390/antiox11112112 https://doaj.org/article/1c9f1746d6a446e0be895dbf4eb975ed |
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ftdoajarticles:oai:doaj.org/article:1c9f1746d6a446e0be895dbf4eb975ed 2023-10-01T03:49:51+02:00 Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack ( Theragra chalcogramma ) Skin Ju Eun Lee Sang-Kyu Noh Mi Jeong Kim 2022-10-01T00:00:00Z https://doi.org/10.3390/antiox11112112 https://doaj.org/article/1c9f1746d6a446e0be895dbf4eb975ed EN eng MDPI AG https://www.mdpi.com/2076-3921/11/11/2112 https://doaj.org/toc/2076-3921 doi:10.3390/antiox11112112 2076-3921 https://doaj.org/article/1c9f1746d6a446e0be895dbf4eb975ed Antioxidants, Vol 11, Iss 2112, p 2112 (2022) Alaska pollock skin collagen hydrolysate ultrasound assisted extraction enzyme assisted extraction antioxidant properties microstructure Therapeutics. Pharmacology RM1-950 article 2022 ftdoajarticles https://doi.org/10.3390/antiox11112112 2023-09-03T00:45:20Z Collagen hydrolysate were extracted from Alaska pollock skin using enzymatic (EAE), ultrasound (UAE), or combination of enzymatic and ultrasound (EAE+UAE) treatment. Control (C) was not treated with enzymatic or ultrasound. The extracts from C, EAE, UAE, and EAE+UAE were fractionated with ≤3, 3–10, 10–30, and ≥30 kDa. Each fraction was evaluated for biological activity and structural properties. All fractions contained high levels of glycine and proline. The ≤3 kDa fraction of control and ultrasound-assisted extracts exhibited the highest antioxidant activity as measured using Trolox equivalent antioxidant capacity, ferric ion reducing antioxidant power, oxygen radical absorbance capacity, and an assay on the inhibition of nitric oxide production by LPS-induced macrophages. The structurally digested collagen was evaluated using FTIR spectra and SDS-PAGE after Alcalase ® and ultrasound treatments. The microstructure of collagen hydrolysate was assessed using SEM microscopy; the surface morphology was altered according to fraction size and extraction conditions. Overall, it was determined that enzyme treatment in combination with ultrasound is the most effective procedure for obtaining digested collagen hydrolysate, which could be used to further improve biotechnological processing for the addition of value to marine production chains in the future. Article in Journal/Newspaper alaska pollock Theragra chalcogramma Alaska Directory of Open Access Journals: DOAJ Articles Antioxidants 11 11 2112 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Alaska pollock skin collagen hydrolysate ultrasound assisted extraction enzyme assisted extraction antioxidant properties microstructure Therapeutics. Pharmacology RM1-950 |
spellingShingle |
Alaska pollock skin collagen hydrolysate ultrasound assisted extraction enzyme assisted extraction antioxidant properties microstructure Therapeutics. Pharmacology RM1-950 Ju Eun Lee Sang-Kyu Noh Mi Jeong Kim Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack ( Theragra chalcogramma ) Skin |
topic_facet |
Alaska pollock skin collagen hydrolysate ultrasound assisted extraction enzyme assisted extraction antioxidant properties microstructure Therapeutics. Pharmacology RM1-950 |
description |
Collagen hydrolysate were extracted from Alaska pollock skin using enzymatic (EAE), ultrasound (UAE), or combination of enzymatic and ultrasound (EAE+UAE) treatment. Control (C) was not treated with enzymatic or ultrasound. The extracts from C, EAE, UAE, and EAE+UAE were fractionated with ≤3, 3–10, 10–30, and ≥30 kDa. Each fraction was evaluated for biological activity and structural properties. All fractions contained high levels of glycine and proline. The ≤3 kDa fraction of control and ultrasound-assisted extracts exhibited the highest antioxidant activity as measured using Trolox equivalent antioxidant capacity, ferric ion reducing antioxidant power, oxygen radical absorbance capacity, and an assay on the inhibition of nitric oxide production by LPS-induced macrophages. The structurally digested collagen was evaluated using FTIR spectra and SDS-PAGE after Alcalase ® and ultrasound treatments. The microstructure of collagen hydrolysate was assessed using SEM microscopy; the surface morphology was altered according to fraction size and extraction conditions. Overall, it was determined that enzyme treatment in combination with ultrasound is the most effective procedure for obtaining digested collagen hydrolysate, which could be used to further improve biotechnological processing for the addition of value to marine production chains in the future. |
format |
Article in Journal/Newspaper |
author |
Ju Eun Lee Sang-Kyu Noh Mi Jeong Kim |
author_facet |
Ju Eun Lee Sang-Kyu Noh Mi Jeong Kim |
author_sort |
Ju Eun Lee |
title |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack ( Theragra chalcogramma ) Skin |
title_short |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack ( Theragra chalcogramma ) Skin |
title_full |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack ( Theragra chalcogramma ) Skin |
title_fullStr |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack ( Theragra chalcogramma ) Skin |
title_full_unstemmed |
Effects of Enzymatic- and Ultrasound-Assisted Extraction on Physicochemical and Antioxidant Properties of Collagen Hydrolysate Fractions from Alaska Pollack ( Theragra chalcogramma ) Skin |
title_sort |
effects of enzymatic- and ultrasound-assisted extraction on physicochemical and antioxidant properties of collagen hydrolysate fractions from alaska pollack ( theragra chalcogramma ) skin |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/antiox11112112 https://doaj.org/article/1c9f1746d6a446e0be895dbf4eb975ed |
genre |
alaska pollock Theragra chalcogramma Alaska |
genre_facet |
alaska pollock Theragra chalcogramma Alaska |
op_source |
Antioxidants, Vol 11, Iss 2112, p 2112 (2022) |
op_relation |
https://www.mdpi.com/2076-3921/11/11/2112 https://doaj.org/toc/2076-3921 doi:10.3390/antiox11112112 2076-3921 https://doaj.org/article/1c9f1746d6a446e0be895dbf4eb975ed |
op_doi |
https://doi.org/10.3390/antiox11112112 |
container_title |
Antioxidants |
container_volume |
11 |
container_issue |
11 |
container_start_page |
2112 |
_version_ |
1778535290924695552 |