Mass Spectrometry Analysis of Shark Skin Proteins
The mucus layer covering the skin of fish has several roles, including protection against pathogens and mechanical damage in which proteins play a key role. While proteins in the skin mucus layer of various common bony fish species have been explored, the proteins of shark skin mucus remain unexplor...
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ftdoajarticles:oai:doaj.org/article:1c81c13d91b04b35a2fdaed7245ddb28 2024-01-07T09:47:24+01:00 Mass Spectrometry Analysis of Shark Skin Proteins Etty Bachar-Wikstrom Braham Dhillon Navi Gill Dhillon Lisa Abbo Sara K. Lindén Jakob D. Wikstrom 2023-11-01T00:00:00Z https://doi.org/10.3390/ijms242316954 https://doaj.org/article/1c81c13d91b04b35a2fdaed7245ddb28 EN eng MDPI AG https://www.mdpi.com/1422-0067/24/23/16954 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms242316954 1422-0067 1661-6596 https://doaj.org/article/1c81c13d91b04b35a2fdaed7245ddb28 International Journal of Molecular Sciences, Vol 24, Iss 23, p 16954 (2023) elasmobranchs sharks skin mucin proteins mass spectrometry Biology (General) QH301-705.5 Chemistry QD1-999 article 2023 ftdoajarticles https://doi.org/10.3390/ijms242316954 2023-12-10T01:36:41Z The mucus layer covering the skin of fish has several roles, including protection against pathogens and mechanical damage in which proteins play a key role. While proteins in the skin mucus layer of various common bony fish species have been explored, the proteins of shark skin mucus remain unexplored. In this pilot study, we examine the protein composition of the skin mucus in spiny dogfish sharks and chain catsharks through mass spectrometry (NanoLC-MS/MS). Overall, we identified 206 and 72 proteins in spiny dogfish ( Squalus acanthias) and chain catsharks ( Scyliorhinus retifer ), respectively. Categorization showed that the proteins belonged to diverse biological processes and that most proteins were cellular albeit a significant minority were secreted, indicative of mucosal immune roles. The secreted proteins are reviewed in detail with emphasis on their immune potentials. Moreover, STRING protein–protein association network analysis showed that proteins of closely related shark species were more similar as compared to a more distantly related shark and a bony fish, although there were also significant overlaps. This study contributes to the growing field of molecular shark studies and provides a foundation for further research into the functional roles and potential human biomedical implications of shark skin mucus proteins. Article in Journal/Newspaper spiny dogfish Squalus acanthias Directory of Open Access Journals: DOAJ Articles International Journal of Molecular Sciences 24 23 16954 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
elasmobranchs sharks skin mucin proteins mass spectrometry Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
elasmobranchs sharks skin mucin proteins mass spectrometry Biology (General) QH301-705.5 Chemistry QD1-999 Etty Bachar-Wikstrom Braham Dhillon Navi Gill Dhillon Lisa Abbo Sara K. Lindén Jakob D. Wikstrom Mass Spectrometry Analysis of Shark Skin Proteins |
topic_facet |
elasmobranchs sharks skin mucin proteins mass spectrometry Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
The mucus layer covering the skin of fish has several roles, including protection against pathogens and mechanical damage in which proteins play a key role. While proteins in the skin mucus layer of various common bony fish species have been explored, the proteins of shark skin mucus remain unexplored. In this pilot study, we examine the protein composition of the skin mucus in spiny dogfish sharks and chain catsharks through mass spectrometry (NanoLC-MS/MS). Overall, we identified 206 and 72 proteins in spiny dogfish ( Squalus acanthias) and chain catsharks ( Scyliorhinus retifer ), respectively. Categorization showed that the proteins belonged to diverse biological processes and that most proteins were cellular albeit a significant minority were secreted, indicative of mucosal immune roles. The secreted proteins are reviewed in detail with emphasis on their immune potentials. Moreover, STRING protein–protein association network analysis showed that proteins of closely related shark species were more similar as compared to a more distantly related shark and a bony fish, although there were also significant overlaps. This study contributes to the growing field of molecular shark studies and provides a foundation for further research into the functional roles and potential human biomedical implications of shark skin mucus proteins. |
format |
Article in Journal/Newspaper |
author |
Etty Bachar-Wikstrom Braham Dhillon Navi Gill Dhillon Lisa Abbo Sara K. Lindén Jakob D. Wikstrom |
author_facet |
Etty Bachar-Wikstrom Braham Dhillon Navi Gill Dhillon Lisa Abbo Sara K. Lindén Jakob D. Wikstrom |
author_sort |
Etty Bachar-Wikstrom |
title |
Mass Spectrometry Analysis of Shark Skin Proteins |
title_short |
Mass Spectrometry Analysis of Shark Skin Proteins |
title_full |
Mass Spectrometry Analysis of Shark Skin Proteins |
title_fullStr |
Mass Spectrometry Analysis of Shark Skin Proteins |
title_full_unstemmed |
Mass Spectrometry Analysis of Shark Skin Proteins |
title_sort |
mass spectrometry analysis of shark skin proteins |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/ijms242316954 https://doaj.org/article/1c81c13d91b04b35a2fdaed7245ddb28 |
genre |
spiny dogfish Squalus acanthias |
genre_facet |
spiny dogfish Squalus acanthias |
op_source |
International Journal of Molecular Sciences, Vol 24, Iss 23, p 16954 (2023) |
op_relation |
https://www.mdpi.com/1422-0067/24/23/16954 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms242316954 1422-0067 1661-6596 https://doaj.org/article/1c81c13d91b04b35a2fdaed7245ddb28 |
op_doi |
https://doi.org/10.3390/ijms242316954 |
container_title |
International Journal of Molecular Sciences |
container_volume |
24 |
container_issue |
23 |
container_start_page |
16954 |
_version_ |
1787429398738108416 |