Epithelial Label-Retaining Cells Are Absent during Tooth Cycling in Salmo salar and Polypterus senegalus.
The Atlantic salmon (Salmo salar) and African bichir (Polypterus senegalus) are both actinopterygian fish species that continuously replace their teeth without the involvement of a successional dental lamina. Instead, they share the presence of a middle dental epithelium: an epithelial tier enclosed...
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ftdoajarticles:oai:doaj.org/article:1c74b99920a0407ab6a3562f91e41e52 2023-05-15T15:32:49+02:00 Epithelial Label-Retaining Cells Are Absent during Tooth Cycling in Salmo salar and Polypterus senegalus. Sam Vandenplas Maxime Willems P Eckhard Witten Tom Hansen Per Gunnar Fjelldal Ann Huysseune 2016-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0152870 https://doaj.org/article/1c74b99920a0407ab6a3562f91e41e52 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4822771?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0152870 https://doaj.org/article/1c74b99920a0407ab6a3562f91e41e52 PLoS ONE, Vol 11, Iss 4, p e0152870 (2016) Medicine R Science Q article 2016 ftdoajarticles https://doi.org/10.1371/journal.pone.0152870 2022-12-31T12:21:15Z The Atlantic salmon (Salmo salar) and African bichir (Polypterus senegalus) are both actinopterygian fish species that continuously replace their teeth without the involvement of a successional dental lamina. Instead, they share the presence of a middle dental epithelium: an epithelial tier enclosed by inner and outer dental epithelium. It has been hypothesized that this tier could functionally substitute for a successional dental lamina and might be a potential niche to house epithelial stem cells involved in tooth cycling. Therefore, in this study we performed a BrdU pulse chase experiment on both species to (1) determine the localization and extent of proliferating cells in the dental epithelial layers, (2) describe cell dynamics and (3) investigate if label-retaining cells are present, suggestive for the putative presence of stem cells. Cells proliferate in the middle dental epithelium, outer dental epithelium and cervical loop at the lingual side of the dental organ to form a new tooth germ. Using long chase times, both in S. salar (eight weeks) and P. senegalus (eight weeks and twelve weeks), we could not reveal the presence of label-retaining cells in the dental organ. Immunostaining of P. senegalus dental organs for the transcription factor Sox2, often used as a stem cell marker, labelled cells in the zone of outer dental epithelium which grades into the oral epithelium (ODE transition zone) and the inner dental epithelium of a successor only. The location of Sox2 distribution does not provide evidence for epithelial stem cells in the dental organ and, more specifically, in the middle dental epithelium. Comparison of S. salar and P. senegalus reveals shared traits in tooth cycling and thus advances our understanding of the developmental mechanism that ensures lifelong replacement. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles PLOS ONE 11 4 e0152870 |
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Medicine R Science Q Sam Vandenplas Maxime Willems P Eckhard Witten Tom Hansen Per Gunnar Fjelldal Ann Huysseune Epithelial Label-Retaining Cells Are Absent during Tooth Cycling in Salmo salar and Polypterus senegalus. |
topic_facet |
Medicine R Science Q |
description |
The Atlantic salmon (Salmo salar) and African bichir (Polypterus senegalus) are both actinopterygian fish species that continuously replace their teeth without the involvement of a successional dental lamina. Instead, they share the presence of a middle dental epithelium: an epithelial tier enclosed by inner and outer dental epithelium. It has been hypothesized that this tier could functionally substitute for a successional dental lamina and might be a potential niche to house epithelial stem cells involved in tooth cycling. Therefore, in this study we performed a BrdU pulse chase experiment on both species to (1) determine the localization and extent of proliferating cells in the dental epithelial layers, (2) describe cell dynamics and (3) investigate if label-retaining cells are present, suggestive for the putative presence of stem cells. Cells proliferate in the middle dental epithelium, outer dental epithelium and cervical loop at the lingual side of the dental organ to form a new tooth germ. Using long chase times, both in S. salar (eight weeks) and P. senegalus (eight weeks and twelve weeks), we could not reveal the presence of label-retaining cells in the dental organ. Immunostaining of P. senegalus dental organs for the transcription factor Sox2, often used as a stem cell marker, labelled cells in the zone of outer dental epithelium which grades into the oral epithelium (ODE transition zone) and the inner dental epithelium of a successor only. The location of Sox2 distribution does not provide evidence for epithelial stem cells in the dental organ and, more specifically, in the middle dental epithelium. Comparison of S. salar and P. senegalus reveals shared traits in tooth cycling and thus advances our understanding of the developmental mechanism that ensures lifelong replacement. |
format |
Article in Journal/Newspaper |
author |
Sam Vandenplas Maxime Willems P Eckhard Witten Tom Hansen Per Gunnar Fjelldal Ann Huysseune |
author_facet |
Sam Vandenplas Maxime Willems P Eckhard Witten Tom Hansen Per Gunnar Fjelldal Ann Huysseune |
author_sort |
Sam Vandenplas |
title |
Epithelial Label-Retaining Cells Are Absent during Tooth Cycling in Salmo salar and Polypterus senegalus. |
title_short |
Epithelial Label-Retaining Cells Are Absent during Tooth Cycling in Salmo salar and Polypterus senegalus. |
title_full |
Epithelial Label-Retaining Cells Are Absent during Tooth Cycling in Salmo salar and Polypterus senegalus. |
title_fullStr |
Epithelial Label-Retaining Cells Are Absent during Tooth Cycling in Salmo salar and Polypterus senegalus. |
title_full_unstemmed |
Epithelial Label-Retaining Cells Are Absent during Tooth Cycling in Salmo salar and Polypterus senegalus. |
title_sort |
epithelial label-retaining cells are absent during tooth cycling in salmo salar and polypterus senegalus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pone.0152870 https://doaj.org/article/1c74b99920a0407ab6a3562f91e41e52 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
PLoS ONE, Vol 11, Iss 4, p e0152870 (2016) |
op_relation |
http://europepmc.org/articles/PMC4822771?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0152870 https://doaj.org/article/1c74b99920a0407ab6a3562f91e41e52 |
op_doi |
https://doi.org/10.1371/journal.pone.0152870 |
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PLOS ONE |
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11 |
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4 |
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e0152870 |
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