Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis
The present study was aimed at analyzing the genetic diversity of promising banana ecotypes grown in Kerala. Twenty eight ecotypes of banana were collected from different parts of Kerala. DNA isolated from these was used for RAPD analysis. Six most-promising primers viz., OPA-01, OPA-03, OPA-13, OPB...
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Society for Promotion of Horticulture - Indian Institute of Horticultural Research
2012
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ftdoajarticles:oai:doaj.org/article:1c73c07ca96b4fd694a91f177cfd2beb 2023-09-26T15:16:14+02:00 Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis C Rajamanickam K Rajmohan 2012-06-01T00:00:00Z https://doi.org/10.24154/jhs.v7i1.385 https://doaj.org/article/1c73c07ca96b4fd694a91f177cfd2beb EN eng Society for Promotion of Horticulture - Indian Institute of Horticultural Research https://jhs.iihr.res.in/index.php/jhs/article/view/385 https://doaj.org/toc/0973-354X https://doaj.org/toc/2582-4899 doi:10.24154/jhs.v7i1.385 0973-354X 2582-4899 https://doaj.org/article/1c73c07ca96b4fd694a91f177cfd2beb Journal of Horticultural Sciences, Vol 7, Iss 1 (2012) Banana Ecotypes RAPD Molecular Markers D2 Analysis Genetic Divergence Plant culture SB1-1110 article 2012 ftdoajarticles https://doi.org/10.24154/jhs.v7i1.385 2023-08-27T00:39:07Z The present study was aimed at analyzing the genetic diversity of promising banana ecotypes grown in Kerala. Twenty eight ecotypes of banana were collected from different parts of Kerala. DNA isolated from these was used for RAPD analysis. Six most-promising primers viz., OPA-01, OPA-03, OPA-13, OPB-04, OPB-10 and OPB-12 were used. These yielded 46 scorable bands with an average of 7.66 bands per primer. RAPD data were analyzed statistically and a dendrogram was constructed. Twenty three characters were observed in the twenty eight banana ecotypes and were statistically analyzed as per the method proposed by Mahalanobis (D2). From RAPD dendrogram, it was found that the banana clones clustered into fourteen groups at a distance of 0.200. At a distance of 0.250, 8 out of 12 Nendran (AAB group) ecotypes formed a single cluster at the same distance. Among Palayankodan (AAB group) ecotypes PKNNR, Pisang Ceylon, Motta Poovan, Chandra Bale and Palode Palayankodan grouped together and formed a single cluster. Attu Nendran, Monthan, Robusta, Koonoor Ethan, Ilavazha and Vellapalayankodan formed individual clusters and had maximum genetic divergence. Among diploid clones, Ilavazha (BB group) had maximum genetic divergence. Among triploid clones, Attu Nendran, Robusta, Koonoor Ethan and Vellapalayankodan showed maximum genetic divergence. Among Nendran (AAB group) ecotypes, Attu Nendran and Koonoor Ethan revealed maximum genetic divergence. Among Palayankodan (AAB group) ecotypes, Vellapalayankodan recorded the highest genetic divergence. In D2 analysis too, a similar trend was observed. Article in Journal/Newspaper Attu Directory of Open Access Journals: DOAJ Articles Motta ENVELOPE(143.887,143.887,59.677,59.677) Musa ENVELOPE(9.617,9.617,63.587,63.587) Journal of Horticultural Sciences 7 1 34 40 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Banana Ecotypes RAPD Molecular Markers D2 Analysis Genetic Divergence Plant culture SB1-1110 |
spellingShingle |
Banana Ecotypes RAPD Molecular Markers D2 Analysis Genetic Divergence Plant culture SB1-1110 C Rajamanickam K Rajmohan Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis |
topic_facet |
Banana Ecotypes RAPD Molecular Markers D2 Analysis Genetic Divergence Plant culture SB1-1110 |
description |
The present study was aimed at analyzing the genetic diversity of promising banana ecotypes grown in Kerala. Twenty eight ecotypes of banana were collected from different parts of Kerala. DNA isolated from these was used for RAPD analysis. Six most-promising primers viz., OPA-01, OPA-03, OPA-13, OPB-04, OPB-10 and OPB-12 were used. These yielded 46 scorable bands with an average of 7.66 bands per primer. RAPD data were analyzed statistically and a dendrogram was constructed. Twenty three characters were observed in the twenty eight banana ecotypes and were statistically analyzed as per the method proposed by Mahalanobis (D2). From RAPD dendrogram, it was found that the banana clones clustered into fourteen groups at a distance of 0.200. At a distance of 0.250, 8 out of 12 Nendran (AAB group) ecotypes formed a single cluster at the same distance. Among Palayankodan (AAB group) ecotypes PKNNR, Pisang Ceylon, Motta Poovan, Chandra Bale and Palode Palayankodan grouped together and formed a single cluster. Attu Nendran, Monthan, Robusta, Koonoor Ethan, Ilavazha and Vellapalayankodan formed individual clusters and had maximum genetic divergence. Among diploid clones, Ilavazha (BB group) had maximum genetic divergence. Among triploid clones, Attu Nendran, Robusta, Koonoor Ethan and Vellapalayankodan showed maximum genetic divergence. Among Nendran (AAB group) ecotypes, Attu Nendran and Koonoor Ethan revealed maximum genetic divergence. Among Palayankodan (AAB group) ecotypes, Vellapalayankodan recorded the highest genetic divergence. In D2 analysis too, a similar trend was observed. |
format |
Article in Journal/Newspaper |
author |
C Rajamanickam K Rajmohan |
author_facet |
C Rajamanickam K Rajmohan |
author_sort |
C Rajamanickam |
title |
Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis |
title_short |
Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis |
title_full |
Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis |
title_fullStr |
Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis |
title_full_unstemmed |
Diversity Studies in Ecotypes of Banana (Musa Spp.) Using Molecular Markers and D2 Analysis |
title_sort |
diversity studies in ecotypes of banana (musa spp.) using molecular markers and d2 analysis |
publisher |
Society for Promotion of Horticulture - Indian Institute of Horticultural Research |
publishDate |
2012 |
url |
https://doi.org/10.24154/jhs.v7i1.385 https://doaj.org/article/1c73c07ca96b4fd694a91f177cfd2beb |
long_lat |
ENVELOPE(143.887,143.887,59.677,59.677) ENVELOPE(9.617,9.617,63.587,63.587) |
geographic |
Motta Musa |
geographic_facet |
Motta Musa |
genre |
Attu |
genre_facet |
Attu |
op_source |
Journal of Horticultural Sciences, Vol 7, Iss 1 (2012) |
op_relation |
https://jhs.iihr.res.in/index.php/jhs/article/view/385 https://doaj.org/toc/0973-354X https://doaj.org/toc/2582-4899 doi:10.24154/jhs.v7i1.385 0973-354X 2582-4899 https://doaj.org/article/1c73c07ca96b4fd694a91f177cfd2beb |
op_doi |
https://doi.org/10.24154/jhs.v7i1.385 |
container_title |
Journal of Horticultural Sciences |
container_volume |
7 |
container_issue |
1 |
container_start_page |
34 |
op_container_end_page |
40 |
_version_ |
1778137407762202624 |