Shotgun metagenomics of fecal samples from children in Peru reveals frequent complex co-infections with multiple Campylobacter species.
Campylobacter spp. are a major cause of bacterial diarrhea worldwide and are associated with high rates of mortality and linear growth faltering in children living in low- to middle-income countries (LMICs). Campylobacter jejuni and Campylobacter coli are most often the causative agents of enteric d...
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ftdoajarticles:oai:doaj.org/article:1c7097da70994558bba7971d2bc97c34 2023-05-15T15:06:47+02:00 Shotgun metagenomics of fecal samples from children in Peru reveals frequent complex co-infections with multiple Campylobacter species. Craig T Parker Francesca Schiaffino Steven Huynh Maribel Paredes Olortegui Pablo Peñataro Yori Paul F Garcia Bardales Tackeshy Pinedo Vasquez Greisi E Curico Huansi Katia Manzanares Villanueva Wagner V Shapiama Lopez Kerry K Cooper Margaret N Kosek 2022-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010815 https://doaj.org/article/1c7097da70994558bba7971d2bc97c34 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010815 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010815 https://doaj.org/article/1c7097da70994558bba7971d2bc97c34 PLoS Neglected Tropical Diseases, Vol 16, Iss 10, p e0010815 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010815 2022-12-30T19:46:11Z Campylobacter spp. are a major cause of bacterial diarrhea worldwide and are associated with high rates of mortality and linear growth faltering in children living in low- to middle-income countries (LMICs). Campylobacter jejuni and Campylobacter coli are most often the causative agents of enteric disease among children in LMICs. However, previous work on a collection of stool samples from children under 2 years of age, living in a low resource community in Peru with either acute diarrheal disease or asymptomatic, were found to be qPCR positive for Campylobacter species but qPCR negative for C. jejuni and C. coli. The goal of this study was to determine if whole-genome shotgun metagenomic sequencing (WSMS) could identify the Campylobacter species within these samples. The Campylobacter species identified in these stool samples included C. jejuni, C. coli, C. upsaliensis, C. concisus, and the potential new species of Campylobacter, "Candidatus Campylobacter infans". Moreover, WSMS results demonstrate that over 65% of the samples represented co-infections with multiple Campylobacter species present in a single stool sample, a novel finding in human populations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 10 e0010815 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Craig T Parker Francesca Schiaffino Steven Huynh Maribel Paredes Olortegui Pablo Peñataro Yori Paul F Garcia Bardales Tackeshy Pinedo Vasquez Greisi E Curico Huansi Katia Manzanares Villanueva Wagner V Shapiama Lopez Kerry K Cooper Margaret N Kosek Shotgun metagenomics of fecal samples from children in Peru reveals frequent complex co-infections with multiple Campylobacter species. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Campylobacter spp. are a major cause of bacterial diarrhea worldwide and are associated with high rates of mortality and linear growth faltering in children living in low- to middle-income countries (LMICs). Campylobacter jejuni and Campylobacter coli are most often the causative agents of enteric disease among children in LMICs. However, previous work on a collection of stool samples from children under 2 years of age, living in a low resource community in Peru with either acute diarrheal disease or asymptomatic, were found to be qPCR positive for Campylobacter species but qPCR negative for C. jejuni and C. coli. The goal of this study was to determine if whole-genome shotgun metagenomic sequencing (WSMS) could identify the Campylobacter species within these samples. The Campylobacter species identified in these stool samples included C. jejuni, C. coli, C. upsaliensis, C. concisus, and the potential new species of Campylobacter, "Candidatus Campylobacter infans". Moreover, WSMS results demonstrate that over 65% of the samples represented co-infections with multiple Campylobacter species present in a single stool sample, a novel finding in human populations. |
format |
Article in Journal/Newspaper |
author |
Craig T Parker Francesca Schiaffino Steven Huynh Maribel Paredes Olortegui Pablo Peñataro Yori Paul F Garcia Bardales Tackeshy Pinedo Vasquez Greisi E Curico Huansi Katia Manzanares Villanueva Wagner V Shapiama Lopez Kerry K Cooper Margaret N Kosek |
author_facet |
Craig T Parker Francesca Schiaffino Steven Huynh Maribel Paredes Olortegui Pablo Peñataro Yori Paul F Garcia Bardales Tackeshy Pinedo Vasquez Greisi E Curico Huansi Katia Manzanares Villanueva Wagner V Shapiama Lopez Kerry K Cooper Margaret N Kosek |
author_sort |
Craig T Parker |
title |
Shotgun metagenomics of fecal samples from children in Peru reveals frequent complex co-infections with multiple Campylobacter species. |
title_short |
Shotgun metagenomics of fecal samples from children in Peru reveals frequent complex co-infections with multiple Campylobacter species. |
title_full |
Shotgun metagenomics of fecal samples from children in Peru reveals frequent complex co-infections with multiple Campylobacter species. |
title_fullStr |
Shotgun metagenomics of fecal samples from children in Peru reveals frequent complex co-infections with multiple Campylobacter species. |
title_full_unstemmed |
Shotgun metagenomics of fecal samples from children in Peru reveals frequent complex co-infections with multiple Campylobacter species. |
title_sort |
shotgun metagenomics of fecal samples from children in peru reveals frequent complex co-infections with multiple campylobacter species. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010815 https://doaj.org/article/1c7097da70994558bba7971d2bc97c34 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 10, p e0010815 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010815 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010815 https://doaj.org/article/1c7097da70994558bba7971d2bc97c34 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010815 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
10 |
container_start_page |
e0010815 |
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