Phylogenetic analysis of Dengue-2 serotypes circulating in mangroves in Northern Cordoba, Colombia
Abstract INTRODUCTION: In this study, we aimed to identify DENV-2 subtypes in Aedes aegypti pools collected between 2011 and 2017 in a rural area of Northern Cordoba, Colombia (“La Balsa”). METHODS: RT-PCR was performed to analyze the capsid/pre-membrane region (C-PrM). Sequencing and phylogenetic b...
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ftdoajarticles:oai:doaj.org/article:1c4cd27e19304e82aa4ced7b4ab6d425 2023-05-15T15:01:34+02:00 Phylogenetic analysis of Dengue-2 serotypes circulating in mangroves in Northern Cordoba, Colombia Richard Hoyos-López Maria Claudia Atencia-Pineda Juan Carlos Gallego-Gómez https://doi.org/10.1590/0037-8682-0060-2019 https://doaj.org/article/1c4cd27e19304e82aa4ced7b4ab6d425 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100665&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0060-2019 https://doaj.org/article/1c4cd27e19304e82aa4ced7b4ab6d425 Revista da Sociedade Brasileira de Medicina Tropical, Vol 52, Iss 0 DENV-2 Genotypes Phylogenetic Aedes aegypti Colombia Arctic medicine. Tropical medicine RC955-962 article ftdoajarticles https://doi.org/10.1590/0037-8682-0060-2019 2022-12-31T02:02:34Z Abstract INTRODUCTION: In this study, we aimed to identify DENV-2 subtypes in Aedes aegypti pools collected between 2011 and 2017 in a rural area of Northern Cordoba, Colombia (“La Balsa”). METHODS: RT-PCR was performed to analyze the capsid/pre-membrane region (C-PrM). Sequencing and phylogenetic bayesian inference using reference DENV-2 sequences were performed. RESULTS: Twelve pools that tested positive for DENV-2 were characterized based on the C-PrM region and grouped under the Asian/American clade. CONCLUSIONS: This study is the first to report the DENV-2 Asian-American subtype in a rural area of Cordoba region, which is associated with severe dengue and local epidemics. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 52 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
DENV-2 Genotypes Phylogenetic Aedes aegypti Colombia Arctic medicine. Tropical medicine RC955-962 |
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DENV-2 Genotypes Phylogenetic Aedes aegypti Colombia Arctic medicine. Tropical medicine RC955-962 Richard Hoyos-López Maria Claudia Atencia-Pineda Juan Carlos Gallego-Gómez Phylogenetic analysis of Dengue-2 serotypes circulating in mangroves in Northern Cordoba, Colombia |
topic_facet |
DENV-2 Genotypes Phylogenetic Aedes aegypti Colombia Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION: In this study, we aimed to identify DENV-2 subtypes in Aedes aegypti pools collected between 2011 and 2017 in a rural area of Northern Cordoba, Colombia (“La Balsa”). METHODS: RT-PCR was performed to analyze the capsid/pre-membrane region (C-PrM). Sequencing and phylogenetic bayesian inference using reference DENV-2 sequences were performed. RESULTS: Twelve pools that tested positive for DENV-2 were characterized based on the C-PrM region and grouped under the Asian/American clade. CONCLUSIONS: This study is the first to report the DENV-2 Asian-American subtype in a rural area of Cordoba region, which is associated with severe dengue and local epidemics. |
format |
Article in Journal/Newspaper |
author |
Richard Hoyos-López Maria Claudia Atencia-Pineda Juan Carlos Gallego-Gómez |
author_facet |
Richard Hoyos-López Maria Claudia Atencia-Pineda Juan Carlos Gallego-Gómez |
author_sort |
Richard Hoyos-López |
title |
Phylogenetic analysis of Dengue-2 serotypes circulating in mangroves in Northern Cordoba, Colombia |
title_short |
Phylogenetic analysis of Dengue-2 serotypes circulating in mangroves in Northern Cordoba, Colombia |
title_full |
Phylogenetic analysis of Dengue-2 serotypes circulating in mangroves in Northern Cordoba, Colombia |
title_fullStr |
Phylogenetic analysis of Dengue-2 serotypes circulating in mangroves in Northern Cordoba, Colombia |
title_full_unstemmed |
Phylogenetic analysis of Dengue-2 serotypes circulating in mangroves in Northern Cordoba, Colombia |
title_sort |
phylogenetic analysis of dengue-2 serotypes circulating in mangroves in northern cordoba, colombia |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
url |
https://doi.org/10.1590/0037-8682-0060-2019 https://doaj.org/article/1c4cd27e19304e82aa4ced7b4ab6d425 |
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Arctic |
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Arctic |
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Arctic |
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Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 52, Iss 0 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822019000100665&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0060-2019 https://doaj.org/article/1c4cd27e19304e82aa4ced7b4ab6d425 |
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https://doi.org/10.1590/0037-8682-0060-2019 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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52 |
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