Sequence analysis of dolphin ferritin H and L subunits and possible iron-dependent translational control of dolphin ferritin gene
Abstract Background Iron-storage protein, ferritin plays a central role in iron metabolism. Ferritin has dual function to store iron and segregate iron for protection of iron-catalyzed reactive oxygen species. Tissue ferritin is composed of two kinds of subunits (H: heavy chain or heart-type subunit...
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ftdoajarticles:oai:doaj.org/article:1c46ad222a6d47869d0963b610fd0f67 2023-05-15T16:00:30+02:00 Sequence analysis of dolphin ferritin H and L subunits and possible iron-dependent translational control of dolphin ferritin gene Sasaki Yukako Takai Shinji Watanabe Kiyotaka Takaesu Azusa Orino Koichi 2008-10-01T00:00:00Z https://doi.org/10.1186/1751-0147-50-42 https://doaj.org/article/1c46ad222a6d47869d0963b610fd0f67 EN eng BMC http://www.actavetscand.com/content/50/1/42 https://doaj.org/toc/1751-0147 doi:10.1186/1751-0147-50-42 1751-0147 https://doaj.org/article/1c46ad222a6d47869d0963b610fd0f67 Acta Veterinaria Scandinavica, Vol 50, Iss 1, p 42 (2008) Veterinary medicine SF600-1100 article 2008 ftdoajarticles https://doi.org/10.1186/1751-0147-50-42 2022-12-31T08:10:17Z Abstract Background Iron-storage protein, ferritin plays a central role in iron metabolism. Ferritin has dual function to store iron and segregate iron for protection of iron-catalyzed reactive oxygen species. Tissue ferritin is composed of two kinds of subunits (H: heavy chain or heart-type subunit; L: light chain or liver-type subunit). Ferritin gene expression is controlled at translational level in iron-dependent manner or at transcriptional level in iron-independent manner. However, sequencing analysis of marine mammalian ferritin subunits has not yet been performed fully. The purpose of this study is to reveal cDNA-derived amino acid sequences of cetacean ferritin H and L subunits, and demonstrate the possibility of expression of these subunits, especially H subunit, by iron. Methods Sequence analyses of cetacean ferritin H and L subunits were performed by direct sequencing of polymerase chain reaction (PCR) fragments from cDNAs generated via reverse transcription-PCR of leukocyte total RNA prepared from blood samples of six different dolphin species ( Pseudorca crassidens , Lagenorhynchus obliquidens , Grampus griseus , Globicephala macrorhynchus , Tursiops truncatus , and Delphinapterus leucas ). The putative iron-responsive element sequence in the 5'-untranslated region of the six different dolphin species was revealed by direct sequencing of PCR fragments obtained using leukocyte genomic DNA. Results Dolphin H and L subunits consist of 182 and 174 amino acids, respectively, and amino acid sequence identities of ferritin subunits among these dolphins are highly conserved (H: 99–100%, (99→98) L: 98–100%). The conserved 28 bp IRE sequence was located -144 bp upstream from the initiation codon in the six different dolphin species. Conclusion These results indicate that six different dolphin species have conserved ferritin sequences, and suggest that these genes are iron-dependently expressed. Article in Journal/Newspaper Delphinapterus leucas Directory of Open Access Journals: DOAJ Articles Acta Veterinaria Scandinavica 50 1 42 |
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Veterinary medicine SF600-1100 |
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Veterinary medicine SF600-1100 Sasaki Yukako Takai Shinji Watanabe Kiyotaka Takaesu Azusa Orino Koichi Sequence analysis of dolphin ferritin H and L subunits and possible iron-dependent translational control of dolphin ferritin gene |
topic_facet |
Veterinary medicine SF600-1100 |
description |
Abstract Background Iron-storage protein, ferritin plays a central role in iron metabolism. Ferritin has dual function to store iron and segregate iron for protection of iron-catalyzed reactive oxygen species. Tissue ferritin is composed of two kinds of subunits (H: heavy chain or heart-type subunit; L: light chain or liver-type subunit). Ferritin gene expression is controlled at translational level in iron-dependent manner or at transcriptional level in iron-independent manner. However, sequencing analysis of marine mammalian ferritin subunits has not yet been performed fully. The purpose of this study is to reveal cDNA-derived amino acid sequences of cetacean ferritin H and L subunits, and demonstrate the possibility of expression of these subunits, especially H subunit, by iron. Methods Sequence analyses of cetacean ferritin H and L subunits were performed by direct sequencing of polymerase chain reaction (PCR) fragments from cDNAs generated via reverse transcription-PCR of leukocyte total RNA prepared from blood samples of six different dolphin species ( Pseudorca crassidens , Lagenorhynchus obliquidens , Grampus griseus , Globicephala macrorhynchus , Tursiops truncatus , and Delphinapterus leucas ). The putative iron-responsive element sequence in the 5'-untranslated region of the six different dolphin species was revealed by direct sequencing of PCR fragments obtained using leukocyte genomic DNA. Results Dolphin H and L subunits consist of 182 and 174 amino acids, respectively, and amino acid sequence identities of ferritin subunits among these dolphins are highly conserved (H: 99–100%, (99→98) L: 98–100%). The conserved 28 bp IRE sequence was located -144 bp upstream from the initiation codon in the six different dolphin species. Conclusion These results indicate that six different dolphin species have conserved ferritin sequences, and suggest that these genes are iron-dependently expressed. |
format |
Article in Journal/Newspaper |
author |
Sasaki Yukako Takai Shinji Watanabe Kiyotaka Takaesu Azusa Orino Koichi |
author_facet |
Sasaki Yukako Takai Shinji Watanabe Kiyotaka Takaesu Azusa Orino Koichi |
author_sort |
Sasaki Yukako |
title |
Sequence analysis of dolphin ferritin H and L subunits and possible iron-dependent translational control of dolphin ferritin gene |
title_short |
Sequence analysis of dolphin ferritin H and L subunits and possible iron-dependent translational control of dolphin ferritin gene |
title_full |
Sequence analysis of dolphin ferritin H and L subunits and possible iron-dependent translational control of dolphin ferritin gene |
title_fullStr |
Sequence analysis of dolphin ferritin H and L subunits and possible iron-dependent translational control of dolphin ferritin gene |
title_full_unstemmed |
Sequence analysis of dolphin ferritin H and L subunits and possible iron-dependent translational control of dolphin ferritin gene |
title_sort |
sequence analysis of dolphin ferritin h and l subunits and possible iron-dependent translational control of dolphin ferritin gene |
publisher |
BMC |
publishDate |
2008 |
url |
https://doi.org/10.1186/1751-0147-50-42 https://doaj.org/article/1c46ad222a6d47869d0963b610fd0f67 |
genre |
Delphinapterus leucas |
genre_facet |
Delphinapterus leucas |
op_source |
Acta Veterinaria Scandinavica, Vol 50, Iss 1, p 42 (2008) |
op_relation |
http://www.actavetscand.com/content/50/1/42 https://doaj.org/toc/1751-0147 doi:10.1186/1751-0147-50-42 1751-0147 https://doaj.org/article/1c46ad222a6d47869d0963b610fd0f67 |
op_doi |
https://doi.org/10.1186/1751-0147-50-42 |
container_title |
Acta Veterinaria Scandinavica |
container_volume |
50 |
container_issue |
1 |
container_start_page |
42 |
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1766396490562928640 |