TNF-α blockade suppresses pericystic inflammation following anthelmintic treatment in porcine neurocysticercosis.
Neurocysticercosis (NCC) is an infection of the brain with the larval cyst of the tapeworm, Taenia solium. Cysticidal treatment induces parasite killing resulting in a post inflammatory response and seizures, which generally requires corticosteroid treatment to control inflammation. The nature of th...
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ftdoajarticles:oai:doaj.org/article:1bcb91374bc944f696042a01ea5e02e0 2023-05-15T15:16:34+02:00 TNF-α blockade suppresses pericystic inflammation following anthelmintic treatment in porcine neurocysticercosis. Siddhartha Mahanty Miguel A Orrego Carla Cangalaya M Paz Adrianzen Gianfranco Arroyo Juan Calcina Armando E Gonzalez Héctor H García Cristina Guerra-Giraldez Theodore E Nash Cysticercosis Working Group in Peru 2017-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006059 https://doaj.org/article/1bcb91374bc944f696042a01ea5e02e0 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5708608?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006059 https://doaj.org/article/1bcb91374bc944f696042a01ea5e02e0 PLoS Neglected Tropical Diseases, Vol 11, Iss 11, p e0006059 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006059 2022-12-31T14:40:46Z Neurocysticercosis (NCC) is an infection of the brain with the larval cyst of the tapeworm, Taenia solium. Cysticidal treatment induces parasite killing resulting in a post inflammatory response and seizures, which generally requires corticosteroid treatment to control inflammation. The nature of this response and how to best control it is unclear. We investigated the anti-inflammatory effects of pretreatment with etanercept (ETN), an anti-tumor necrosis factor agent, or dexamethasone (DEX), a high potency corticosteroid, on the post treatment inflammatory response in naturally infected pigs with neurocysticercosis after a single dose of the cysticidal drug praziquantel (PZQ).We followed the methods from a previously developed treatment model of NCC in naturally infected swine. The four study groups of infected pigs included 3 groups treated with PZQ on day 0: PZQ-treated alone (100 mg/kg PO; n = 9), pretreated with dexamethasone (DEX, 0.2 mg/kg IM administered on days -1, +1 and +3; n = 6), and pretreated with etanercept (ETN, 25 mg IM per animal on days -7 and 0; n = 6). The fourth group remained untreated (n = 3). As measured by quantitative RT-PCR, ETN pretreatment depressed transcription of a wide range of proinflammatory, regulatory and matrix protease encoding genes at 120 hr post PZQ treatment in capsules of cysts that demonstrated extravasated Evans Blue (EB) (a measure of blood brain barrier dysfunction) compared to animals not receiving ETN. Transcription was significantly depressed for the proinflammatory genes tumor necrosis factor (TNF)-α, and interferon (IFN)-γ; the inflammation regulating genes cytotoxic T-lymphocyte-associated protein (CTLA)4, interleukin (IL)-13 and transforming growth factor (TGF)-β; the tissue remodeling genes matrix metalloprotease (MMP)1 and 9, tissue inhibitors of metalloproteases (TIMP)1 and 2, and the genes regulating endothelial function vascular endothelial growth factor (VEGF)1, angiopoietin (Ang)1, Ang 2, and platelet endothelial cell adhesion molecule (PECAM)-1. In ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 11 e0006059 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Siddhartha Mahanty Miguel A Orrego Carla Cangalaya M Paz Adrianzen Gianfranco Arroyo Juan Calcina Armando E Gonzalez Héctor H García Cristina Guerra-Giraldez Theodore E Nash Cysticercosis Working Group in Peru TNF-α blockade suppresses pericystic inflammation following anthelmintic treatment in porcine neurocysticercosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Neurocysticercosis (NCC) is an infection of the brain with the larval cyst of the tapeworm, Taenia solium. Cysticidal treatment induces parasite killing resulting in a post inflammatory response and seizures, which generally requires corticosteroid treatment to control inflammation. The nature of this response and how to best control it is unclear. We investigated the anti-inflammatory effects of pretreatment with etanercept (ETN), an anti-tumor necrosis factor agent, or dexamethasone (DEX), a high potency corticosteroid, on the post treatment inflammatory response in naturally infected pigs with neurocysticercosis after a single dose of the cysticidal drug praziquantel (PZQ).We followed the methods from a previously developed treatment model of NCC in naturally infected swine. The four study groups of infected pigs included 3 groups treated with PZQ on day 0: PZQ-treated alone (100 mg/kg PO; n = 9), pretreated with dexamethasone (DEX, 0.2 mg/kg IM administered on days -1, +1 and +3; n = 6), and pretreated with etanercept (ETN, 25 mg IM per animal on days -7 and 0; n = 6). The fourth group remained untreated (n = 3). As measured by quantitative RT-PCR, ETN pretreatment depressed transcription of a wide range of proinflammatory, regulatory and matrix protease encoding genes at 120 hr post PZQ treatment in capsules of cysts that demonstrated extravasated Evans Blue (EB) (a measure of blood brain barrier dysfunction) compared to animals not receiving ETN. Transcription was significantly depressed for the proinflammatory genes tumor necrosis factor (TNF)-α, and interferon (IFN)-γ; the inflammation regulating genes cytotoxic T-lymphocyte-associated protein (CTLA)4, interleukin (IL)-13 and transforming growth factor (TGF)-β; the tissue remodeling genes matrix metalloprotease (MMP)1 and 9, tissue inhibitors of metalloproteases (TIMP)1 and 2, and the genes regulating endothelial function vascular endothelial growth factor (VEGF)1, angiopoietin (Ang)1, Ang 2, and platelet endothelial cell adhesion molecule (PECAM)-1. In ... |
format |
Article in Journal/Newspaper |
author |
Siddhartha Mahanty Miguel A Orrego Carla Cangalaya M Paz Adrianzen Gianfranco Arroyo Juan Calcina Armando E Gonzalez Héctor H García Cristina Guerra-Giraldez Theodore E Nash Cysticercosis Working Group in Peru |
author_facet |
Siddhartha Mahanty Miguel A Orrego Carla Cangalaya M Paz Adrianzen Gianfranco Arroyo Juan Calcina Armando E Gonzalez Héctor H García Cristina Guerra-Giraldez Theodore E Nash Cysticercosis Working Group in Peru |
author_sort |
Siddhartha Mahanty |
title |
TNF-α blockade suppresses pericystic inflammation following anthelmintic treatment in porcine neurocysticercosis. |
title_short |
TNF-α blockade suppresses pericystic inflammation following anthelmintic treatment in porcine neurocysticercosis. |
title_full |
TNF-α blockade suppresses pericystic inflammation following anthelmintic treatment in porcine neurocysticercosis. |
title_fullStr |
TNF-α blockade suppresses pericystic inflammation following anthelmintic treatment in porcine neurocysticercosis. |
title_full_unstemmed |
TNF-α blockade suppresses pericystic inflammation following anthelmintic treatment in porcine neurocysticercosis. |
title_sort |
tnf-α blockade suppresses pericystic inflammation following anthelmintic treatment in porcine neurocysticercosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0006059 https://doaj.org/article/1bcb91374bc944f696042a01ea5e02e0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 11, p e0006059 (2017) |
op_relation |
http://europepmc.org/articles/PMC5708608?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006059 https://doaj.org/article/1bcb91374bc944f696042a01ea5e02e0 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006059 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
11 |
container_start_page |
e0006059 |
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1766346854552829952 |