Comparación de los niveles séricos de óxido nítrico y malondialdehído en pacientes con pie diabético con y sin infección

• Main Authors: Hernández Arteaga M, Núñez R, Godoy N, Socarras E, González Z, Chávez J, Cano C, Bermúdez VSuárez G, Suárez G
• Format: Article in Journal/Newspaper
• Language: English; Spanish
• Published: Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales 2002
• Subjects: Nitric oxide; malondialdehyde; diabetic foot; infection; Óxido nítrico; malondialdehído; pie diabético; infección; Arctic medicine. Tropical medicine; RC955-962; Public aspects of medicine; RA1-1270; Arctic
• Online Access: https://doaj.org/article/1bbc873bd0ee4ef6a5ae35d504ba308a

Abstract: Pathogenesis of diabetic foot infections include polineuropathy, macro and microvascular disease and neutrophil functional deficit. Endothelium dependent vasodilatation related to Nitric Oxide (NO) production is compromised in diabetics predisposed to foot ulceration. NO is also produced when neutrophils and macrophages are stimulated by cytokines or bacterial lipopolysaccarides through the inducible nitric oxide synthase (iNOS), exerting microbicide activity. Lipid peroxidation reflected by Malondialdehyde (MDA) production, is associated with diabetic microangiopathy development. To compare serum NO and MDA levels in patients with infected and non-infected diabetic foot, 27 type 2 diabetics, 38 to 65 years old were studied, and divided into three groups: 8 control subjects without foot affection (A), 10 with non-infected diabetic foot (B) and 9 with infected diabetic foot (C). NO was determined by diazotization assay and MDA by thiobarbituric acid assay. B and C showed lower NO levels (p = 7.636 x 10-5 and p = 0,006) compared with A group. NO was higher in the infected group than in the non-infected (p = 0,05), nevertheless, the obtained values do not coincide with the expected values considering the big amount of NO produced through iNOS, it suggests another defect of diabetic’s leukocyte. There were no significant differences in MDA between the groups, indicating absence of relevant oxidative stress. Resumen: La patogenia de las infecciones del pie diabético involucra la polineuropatía, la enfermedad macro y microvascular y el déficit funcional de los neutrófilos. La vasodilatación dependiente del endotelio, relacionada con el Óxido Nítrico (NO), está comprometida en diabéticos predispuestos a ulceración del pie. El NO, es producido también por neutrófilos y macrófagos estimulados por citoquinas o lipopolisacáridos bacterianos mediante la sintetasa de NO inducible (iNOS), ejerciendo actividad microbicida. La peroxidación lipídica reflejada por la producción de Malondialdehido (MDA), está implicada ...