High gene expression of inflammatory markers and IL-17A correlates with severity of injection site reactions of Atlantic salmon vaccinated with oil-adjuvanted vaccines

• Published in: BMC Genomics
• Main Authors: Koop Ben F, Marjara Inderjit S, Cooper Glenn A, Mutoloki Stephen, Evensen Øystein
• Format: Article in Journal/Newspaper
• Language: English
• Published: BMC 2010
• Subjects: Biotechnology; TP248.13-248.65; Genetics; QH426-470; Gata; Atlantic salmon
• Online Access: https://doi.org/10.1186/1471-2164-11-336; https://doaj.org/article/1b7605218e70411db24635d843eda306

Abstract Background Two decades after the introduction of oil-based vaccines in the control of bacterial and viral diseases in farmed salmonids, the mechanisms of induced side effects manifested as intra-abdominal granulomas remain unresolved. Side effects have been associated with generation of auto-antibodies and autoimmunity but the underlying profile of inflammatory and immune response has not been characterized. This study was undertaken with the aim to elucidate the inflammatory and immune mechanisms of granuloma formation at gene expression level associated with high and low side effect (granuloma) indices. Groups of Atlantic salmon parr were injected intraperitoneally with oil-adjuvanted vaccines containing either high or low concentrations of Aeromonas salmonicida or Moritella viscosa antigens in order to induce polarized (severe and mild) granulomatous reactions. The established granulomatous reactions were confirmed by gross and histological methods at 3 months post vaccination when responses were known to have matured. The corresponding gene expression patterns in the head kidneys were profiled using salmonid cDNA microarrays followed by validation by real-time quantitative PCR (qPCR). qPCR was also used to examine the expression of additional genes known to be important in the adaptive immune response. Results Granulomatous lesions were observed in all vaccinated fish. The presence of severe granulomas was associated with a profile of up-regulation of innate immunity-related genes such as complement factors C1q and C6, mannose binding protein, lysozyme C, C-type lectin receptor, CD209, Cathepsin D, CD63, LECT-2, CC chemokine and metallothionein. In addition, TGF-β (p = 0.001), IL-17A (p = 0.007) and its receptor (IL-17AR) (p = 0.009) representing T H 17 were significantly up-regulated in the group with severe granulomas as were arginase and IgM. None of the genes directly reflective of T H 1 T cell lineage (IFN-γ, CD4) or T H 2 (GATA-3) responses were differentially expressed. Conclusions ...