Colwellia psychrerythraea strains from distant deep sea basins show adaptation to local conditions
Many studies have shown that microbes, which share nearly identical 16S rRNA genes, can have highly divergent genomes. Microbes from distinct parts of the ocean also exhibit biogeographic patterning. Here we seek to better understand how certain microbes from the same species have adapted for growth...
Published in: | Frontiers in Environmental Science |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2016
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fenvs.2016.00033 https://doaj.org/article/1b2989203e3f479fb9da38382e175782 |
id |
ftdoajarticles:oai:doaj.org/article:1b2989203e3f479fb9da38382e175782 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:1b2989203e3f479fb9da38382e175782 2023-05-15T15:10:53+02:00 Colwellia psychrerythraea strains from distant deep sea basins show adaptation to local conditions Stephen M Techtmann Kathleen S Fitzgerald Savannah C Stelling Dominique C Joyner Sagar M Uttukar Austin P Harris Noor K Alshibli Steven D. Brown Terry C Hazen 2016-05-01T00:00:00Z https://doi.org/10.3389/fenvs.2016.00033 https://doaj.org/article/1b2989203e3f479fb9da38382e175782 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fenvs.2016.00033/full https://doaj.org/toc/2296-665X 2296-665X doi:10.3389/fenvs.2016.00033 https://doaj.org/article/1b2989203e3f479fb9da38382e175782 Frontiers in Environmental Science, Vol 4 (2016) Bacteria Genomics Mediterranean Sea 16S rRNA phenotype Genotype Environmental sciences GE1-350 article 2016 ftdoajarticles https://doi.org/10.3389/fenvs.2016.00033 2022-12-31T04:31:39Z Many studies have shown that microbes, which share nearly identical 16S rRNA genes, can have highly divergent genomes. Microbes from distinct parts of the ocean also exhibit biogeographic patterning. Here we seek to better understand how certain microbes from the same species have adapted for growth under local conditions. The phenotypic and genomic heterogeneity of three strains of Colwellia psychrerythraea was investigated in order to understand adaptions to local environments. Colwellia are psychrophilic heterotrophic marine bacteria ubiquitous in cold marine ecosystems. We have recently isolated two Colwellia strains: ND2E from the Eastern Mediterranean and GAB14E from the Great Australian Bight. The 16S rRNA sequence of these two strains were greater than 98.2% identical to the well-characterized C. psychrerythraea 34H, which was isolated from arctic sediments. Salt tolerance, and carbon source utilization profiles for these strains were determined using Biolog Phenotype Microarrays’. These strains exhibited distinct salt tolerance, which was not associated with the salinity of sites of isolation. The carbon source utilization profiles were distinct with less than half of the tested carbon sources being metabolized by all three strains. Whole genome sequencing revealed that the genomes of these three strains were quite diverse with some genomes having up to 1600 strain-specific genes. Many genes involved in degrading strain-specific carbon sources were identified. There appears to be a link between carbon source utilization and location of isolation with distinctions observed between the Colwellia isolate recovered from sediment compared to water column isolates. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Frontiers in Environmental Science 4 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Bacteria Genomics Mediterranean Sea 16S rRNA phenotype Genotype Environmental sciences GE1-350 |
spellingShingle |
Bacteria Genomics Mediterranean Sea 16S rRNA phenotype Genotype Environmental sciences GE1-350 Stephen M Techtmann Kathleen S Fitzgerald Savannah C Stelling Dominique C Joyner Sagar M Uttukar Austin P Harris Noor K Alshibli Steven D. Brown Terry C Hazen Colwellia psychrerythraea strains from distant deep sea basins show adaptation to local conditions |
topic_facet |
Bacteria Genomics Mediterranean Sea 16S rRNA phenotype Genotype Environmental sciences GE1-350 |
description |
Many studies have shown that microbes, which share nearly identical 16S rRNA genes, can have highly divergent genomes. Microbes from distinct parts of the ocean also exhibit biogeographic patterning. Here we seek to better understand how certain microbes from the same species have adapted for growth under local conditions. The phenotypic and genomic heterogeneity of three strains of Colwellia psychrerythraea was investigated in order to understand adaptions to local environments. Colwellia are psychrophilic heterotrophic marine bacteria ubiquitous in cold marine ecosystems. We have recently isolated two Colwellia strains: ND2E from the Eastern Mediterranean and GAB14E from the Great Australian Bight. The 16S rRNA sequence of these two strains were greater than 98.2% identical to the well-characterized C. psychrerythraea 34H, which was isolated from arctic sediments. Salt tolerance, and carbon source utilization profiles for these strains were determined using Biolog Phenotype Microarrays’. These strains exhibited distinct salt tolerance, which was not associated with the salinity of sites of isolation. The carbon source utilization profiles were distinct with less than half of the tested carbon sources being metabolized by all three strains. Whole genome sequencing revealed that the genomes of these three strains were quite diverse with some genomes having up to 1600 strain-specific genes. Many genes involved in degrading strain-specific carbon sources were identified. There appears to be a link between carbon source utilization and location of isolation with distinctions observed between the Colwellia isolate recovered from sediment compared to water column isolates. |
format |
Article in Journal/Newspaper |
author |
Stephen M Techtmann Kathleen S Fitzgerald Savannah C Stelling Dominique C Joyner Sagar M Uttukar Austin P Harris Noor K Alshibli Steven D. Brown Terry C Hazen |
author_facet |
Stephen M Techtmann Kathleen S Fitzgerald Savannah C Stelling Dominique C Joyner Sagar M Uttukar Austin P Harris Noor K Alshibli Steven D. Brown Terry C Hazen |
author_sort |
Stephen M Techtmann |
title |
Colwellia psychrerythraea strains from distant deep sea basins show adaptation to local conditions |
title_short |
Colwellia psychrerythraea strains from distant deep sea basins show adaptation to local conditions |
title_full |
Colwellia psychrerythraea strains from distant deep sea basins show adaptation to local conditions |
title_fullStr |
Colwellia psychrerythraea strains from distant deep sea basins show adaptation to local conditions |
title_full_unstemmed |
Colwellia psychrerythraea strains from distant deep sea basins show adaptation to local conditions |
title_sort |
colwellia psychrerythraea strains from distant deep sea basins show adaptation to local conditions |
publisher |
Frontiers Media S.A. |
publishDate |
2016 |
url |
https://doi.org/10.3389/fenvs.2016.00033 https://doaj.org/article/1b2989203e3f479fb9da38382e175782 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Frontiers in Environmental Science, Vol 4 (2016) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fenvs.2016.00033/full https://doaj.org/toc/2296-665X 2296-665X doi:10.3389/fenvs.2016.00033 https://doaj.org/article/1b2989203e3f479fb9da38382e175782 |
op_doi |
https://doi.org/10.3389/fenvs.2016.00033 |
container_title |
Frontiers in Environmental Science |
container_volume |
4 |
_version_ |
1766341825933606912 |