Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the Amazon basin of Peru
Abstract Background Several of the intended Plasmodium falciparum vaccine candidate antigens are highly polymorphic and could render a vaccine ineffective if their antigenic sites were not represented in the vaccine. In this study, characterization of genetic variability was performed in major B and...
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ftdoajarticles:oai:doaj.org/article:1b0e8c301d994a7db1d2f8522d345184 2023-05-15T15:12:50+02:00 Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the Amazon basin of Peru Lucas Carmen M Escalante Ananias A Branch OraLee H Chenet Stella M Bacon David J 2008-05-01T00:00:00Z https://doi.org/10.1186/1475-2875-7-93 https://doaj.org/article/1b0e8c301d994a7db1d2f8522d345184 EN eng BMC http://www.malariajournal.com/content/7/1/93 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-93 1475-2875 https://doaj.org/article/1b0e8c301d994a7db1d2f8522d345184 Malaria Journal, Vol 7, Iss 1, p 93 (2008) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2008 ftdoajarticles https://doi.org/10.1186/1475-2875-7-93 2022-12-31T13:58:43Z Abstract Background Several of the intended Plasmodium falciparum vaccine candidate antigens are highly polymorphic and could render a vaccine ineffective if their antigenic sites were not represented in the vaccine. In this study, characterization of genetic variability was performed in major B and T-cell epitopes within vaccine candidate antigens in isolates of P. falciparum from Peru. Methods DNA sequencing analysis was completed on 139 isolates of P. falciparum collected from endemic areas of the Amazon basin in Loreto, Peru from years 1998 to 2006. Genetic diversity was determined in immunological important regions in circumsporozoite protein (CSP), merozoite surface protein-1 (MSP-1), apical membrane antigen-1 (AMA-1), liver stage antigen-1 (LSA-1) and thrombospondin-related anonymous protein (TRAP). Alleles identified by DNA sequencing were aligned with the vaccine strain 3D7 and DNA polymorphism analysis and FST study-year pairwise comparisons were done using the DnaSP software. Multilocus analysis (MLA) was performed and average of expected heterozygosity was calculated for each loci and haplotype over time. Results Three different alleles for CSP, seven for MSP-1 Block 2, one for MSP-1 Block 17, three for AMA-1 and for LSA-1 each and one for TRAP were identified. There were 24 different haplotypes in 125 infections with complete locus typing for each gene. Conclusion Characterization of the genetic diversity in Plasmodium isolates from the Amazon Region of Peru showed that P. falciparum T and B cell epitopes in these antigens have polymorphisms more similar to India than to Africa. These findings are helpful in the formulation of a vaccine considering restricted repertoire populations. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 7 1 93 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Lucas Carmen M Escalante Ananias A Branch OraLee H Chenet Stella M Bacon David J Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the Amazon basin of Peru |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Several of the intended Plasmodium falciparum vaccine candidate antigens are highly polymorphic and could render a vaccine ineffective if their antigenic sites were not represented in the vaccine. In this study, characterization of genetic variability was performed in major B and T-cell epitopes within vaccine candidate antigens in isolates of P. falciparum from Peru. Methods DNA sequencing analysis was completed on 139 isolates of P. falciparum collected from endemic areas of the Amazon basin in Loreto, Peru from years 1998 to 2006. Genetic diversity was determined in immunological important regions in circumsporozoite protein (CSP), merozoite surface protein-1 (MSP-1), apical membrane antigen-1 (AMA-1), liver stage antigen-1 (LSA-1) and thrombospondin-related anonymous protein (TRAP). Alleles identified by DNA sequencing were aligned with the vaccine strain 3D7 and DNA polymorphism analysis and FST study-year pairwise comparisons were done using the DnaSP software. Multilocus analysis (MLA) was performed and average of expected heterozygosity was calculated for each loci and haplotype over time. Results Three different alleles for CSP, seven for MSP-1 Block 2, one for MSP-1 Block 17, three for AMA-1 and for LSA-1 each and one for TRAP were identified. There were 24 different haplotypes in 125 infections with complete locus typing for each gene. Conclusion Characterization of the genetic diversity in Plasmodium isolates from the Amazon Region of Peru showed that P. falciparum T and B cell epitopes in these antigens have polymorphisms more similar to India than to Africa. These findings are helpful in the formulation of a vaccine considering restricted repertoire populations. |
format |
Article in Journal/Newspaper |
author |
Lucas Carmen M Escalante Ananias A Branch OraLee H Chenet Stella M Bacon David J |
author_facet |
Lucas Carmen M Escalante Ananias A Branch OraLee H Chenet Stella M Bacon David J |
author_sort |
Lucas Carmen M |
title |
Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the Amazon basin of Peru |
title_short |
Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the Amazon basin of Peru |
title_full |
Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the Amazon basin of Peru |
title_fullStr |
Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the Amazon basin of Peru |
title_full_unstemmed |
Genetic diversity of vaccine candidate antigens in Plasmodium falciparum isolates from the Amazon basin of Peru |
title_sort |
genetic diversity of vaccine candidate antigens in plasmodium falciparum isolates from the amazon basin of peru |
publisher |
BMC |
publishDate |
2008 |
url |
https://doi.org/10.1186/1475-2875-7-93 https://doaj.org/article/1b0e8c301d994a7db1d2f8522d345184 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 7, Iss 1, p 93 (2008) |
op_relation |
http://www.malariajournal.com/content/7/1/93 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-7-93 1475-2875 https://doaj.org/article/1b0e8c301d994a7db1d2f8522d345184 |
op_doi |
https://doi.org/10.1186/1475-2875-7-93 |
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Malaria Journal |
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7 |
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93 |
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