FGF4 independent derivation of trophoblast stem cells from the common vole.
The derivation of stable multipotent trophoblast stem (TS) cell lines from preimplantation, and early postimplantation mouse embryos has been reported previously. FGF4, and its receptor FGFR2, have been identified as embryonic signaling factors responsible for the maintenance of the undifferentiated...
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ftdoajarticles:oai:doaj.org/article:1ac93b650cf44cbca3927376a27ed73e 2023-05-15T15:56:34+02:00 FGF4 independent derivation of trophoblast stem cells from the common vole. Elena V Grigor'eva Alexander I Shevchenko Nina A Mazurok Eugeny A Elisaphenko Antonina I Zhelezova Alexander G Shilov Pavel A Dyban Andrey P Dyban Ekaterina M Noniashvili Sergey Ya Slobodyanyuk Tatyana B Nesterova Neil Brockdorff Suren M Zakian 2009-09-01T00:00:00Z https://doi.org/10.1371/journal.pone.0007161 https://doaj.org/article/1ac93b650cf44cbca3927376a27ed73e EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2744875?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0007161 https://doaj.org/article/1ac93b650cf44cbca3927376a27ed73e PLoS ONE, Vol 4, Iss 9, p e7161 (2009) Medicine R Science Q article 2009 ftdoajarticles https://doi.org/10.1371/journal.pone.0007161 2022-12-31T14:57:41Z The derivation of stable multipotent trophoblast stem (TS) cell lines from preimplantation, and early postimplantation mouse embryos has been reported previously. FGF4, and its receptor FGFR2, have been identified as embryonic signaling factors responsible for the maintenance of the undifferentiated state of multipotent TS cells. Here we report the derivation of stable TS-like cell lines from the vole M. rossiaemeridionalis, in the absence of FGF4 and heparin. Vole TS-like cells are similar to murine TS cells with respect to their morphology, transcription factor gene expression and differentiation in vitro into derivatives of the trophectoderm lineage, and with respect to their ability to invade and erode host tissues, forming haemorrhagic tumours after subcutaneous injection into nude mice. Moreover, vole TS-like cells carry an inactive paternal X chromosome, indicating that they have undergone imprinted X inactivation, which is characteristic of the trophoblast lineage. Our results indicate that an alternative signaling pathway may be responsible for the establishment and stable proliferation of vole TS-like cells. Article in Journal/Newspaper Common vole Directory of Open Access Journals: DOAJ Articles PLoS ONE 4 9 e7161 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Medicine R Science Q |
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Medicine R Science Q Elena V Grigor'eva Alexander I Shevchenko Nina A Mazurok Eugeny A Elisaphenko Antonina I Zhelezova Alexander G Shilov Pavel A Dyban Andrey P Dyban Ekaterina M Noniashvili Sergey Ya Slobodyanyuk Tatyana B Nesterova Neil Brockdorff Suren M Zakian FGF4 independent derivation of trophoblast stem cells from the common vole. |
topic_facet |
Medicine R Science Q |
description |
The derivation of stable multipotent trophoblast stem (TS) cell lines from preimplantation, and early postimplantation mouse embryos has been reported previously. FGF4, and its receptor FGFR2, have been identified as embryonic signaling factors responsible for the maintenance of the undifferentiated state of multipotent TS cells. Here we report the derivation of stable TS-like cell lines from the vole M. rossiaemeridionalis, in the absence of FGF4 and heparin. Vole TS-like cells are similar to murine TS cells with respect to their morphology, transcription factor gene expression and differentiation in vitro into derivatives of the trophectoderm lineage, and with respect to their ability to invade and erode host tissues, forming haemorrhagic tumours after subcutaneous injection into nude mice. Moreover, vole TS-like cells carry an inactive paternal X chromosome, indicating that they have undergone imprinted X inactivation, which is characteristic of the trophoblast lineage. Our results indicate that an alternative signaling pathway may be responsible for the establishment and stable proliferation of vole TS-like cells. |
format |
Article in Journal/Newspaper |
author |
Elena V Grigor'eva Alexander I Shevchenko Nina A Mazurok Eugeny A Elisaphenko Antonina I Zhelezova Alexander G Shilov Pavel A Dyban Andrey P Dyban Ekaterina M Noniashvili Sergey Ya Slobodyanyuk Tatyana B Nesterova Neil Brockdorff Suren M Zakian |
author_facet |
Elena V Grigor'eva Alexander I Shevchenko Nina A Mazurok Eugeny A Elisaphenko Antonina I Zhelezova Alexander G Shilov Pavel A Dyban Andrey P Dyban Ekaterina M Noniashvili Sergey Ya Slobodyanyuk Tatyana B Nesterova Neil Brockdorff Suren M Zakian |
author_sort |
Elena V Grigor'eva |
title |
FGF4 independent derivation of trophoblast stem cells from the common vole. |
title_short |
FGF4 independent derivation of trophoblast stem cells from the common vole. |
title_full |
FGF4 independent derivation of trophoblast stem cells from the common vole. |
title_fullStr |
FGF4 independent derivation of trophoblast stem cells from the common vole. |
title_full_unstemmed |
FGF4 independent derivation of trophoblast stem cells from the common vole. |
title_sort |
fgf4 independent derivation of trophoblast stem cells from the common vole. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2009 |
url |
https://doi.org/10.1371/journal.pone.0007161 https://doaj.org/article/1ac93b650cf44cbca3927376a27ed73e |
genre |
Common vole |
genre_facet |
Common vole |
op_source |
PLoS ONE, Vol 4, Iss 9, p e7161 (2009) |
op_relation |
http://europepmc.org/articles/PMC2744875?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0007161 https://doaj.org/article/1ac93b650cf44cbca3927376a27ed73e |
op_doi |
https://doi.org/10.1371/journal.pone.0007161 |
container_title |
PLoS ONE |
container_volume |
4 |
container_issue |
9 |
container_start_page |
e7161 |
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1766391963816296448 |