Morphological studies in a model for dengue-2 virus infection in mice
One of the main difficulties in studying dengue virus infection in humans and in developing a vaccine is the absence of a suitable animal model which develops the full spectrum of dengue fever, dengue haemorrhagic fever, and dengue shock syndrome. It is our proposal to present morphological aspects...
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ftdoajarticles:oai:doaj.org/article:1aabe40cc2cf4748bdaf33645e0b872f 2023-05-15T15:09:31+02:00 Morphological studies in a model for dengue-2 virus infection in mice Ortrud Monika Barth Débora Ferreira Barreto Marciano Viana Paes Christina Maeda Takiya Angela Teixeira Pinhão Hermann Gonçalves Schatzmayr 2006-12-01T00:00:00Z https://doi.org/10.1590/S0074-02762006000800014 https://doaj.org/article/1aabe40cc2cf4748bdaf33645e0b872f EN eng Instituto Oswaldo Cruz, Ministério da Saúde http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000800014 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02762006000800014 0074-0276 1678-8060 https://doaj.org/article/1aabe40cc2cf4748bdaf33645e0b872f Memórias do Instituto Oswaldo Cruz., Vol 101, Iss 8, Pp 905-915 (2006) dengue-2 virus BALB/c mice liver viremia ultrastructure histopathology Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 article 2006 ftdoajarticles https://doi.org/10.1590/S0074-02762006000800014 2023-01-08T01:33:41Z One of the main difficulties in studying dengue virus infection in humans and in developing a vaccine is the absence of a suitable animal model which develops the full spectrum of dengue fever, dengue haemorrhagic fever, and dengue shock syndrome. It is our proposal to present morphological aspects of an animal model which shows many similarities with the dengue infection in humans. BALB/c mice were intraperitoneally infected with non-neuroadapted dengue virus serotype 2 (DENV-2). Histopathological and morphometrical analyses of liver tissue revealed focal alterations along the infection, reaching wide-ranging portal and centrolobular veins congestion and sinusoidal cell death. Additional ultrastructural observations demonstrated multifocal endothelial injury, platelet recruitment, and alterated hepatocytes. Dengue virus antigen was detected in hepatocytes and in the capillar endothelium of the central lobular vein area. Liver function tests showed high levels of aspartate transaminase and alanine transaminase enzyme activity. Lung tissue showed interstitial pneumonia and mononuclear cells, interseptal oedema, hyperplasia, and hypertrophy of the bronchiolar epithelial cells. DENV-2 led to a transient inflammatory process, but caused focal alterations of the blood-exchange barrier. Viremia was observed from 2nd to 11th day p.i. by isolation of DENV-2 in C6/36 mosquito cell line inoculated with the supernatant of macerated liver, lung, kidney, and cerebellum tissues of the infected mice. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Memórias do Instituto Oswaldo Cruz 101 8 905 915 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
dengue-2 virus BALB/c mice liver viremia ultrastructure histopathology Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
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dengue-2 virus BALB/c mice liver viremia ultrastructure histopathology Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 Ortrud Monika Barth Débora Ferreira Barreto Marciano Viana Paes Christina Maeda Takiya Angela Teixeira Pinhão Hermann Gonçalves Schatzmayr Morphological studies in a model for dengue-2 virus infection in mice |
topic_facet |
dengue-2 virus BALB/c mice liver viremia ultrastructure histopathology Arctic medicine. Tropical medicine RC955-962 Microbiology QR1-502 |
description |
One of the main difficulties in studying dengue virus infection in humans and in developing a vaccine is the absence of a suitable animal model which develops the full spectrum of dengue fever, dengue haemorrhagic fever, and dengue shock syndrome. It is our proposal to present morphological aspects of an animal model which shows many similarities with the dengue infection in humans. BALB/c mice were intraperitoneally infected with non-neuroadapted dengue virus serotype 2 (DENV-2). Histopathological and morphometrical analyses of liver tissue revealed focal alterations along the infection, reaching wide-ranging portal and centrolobular veins congestion and sinusoidal cell death. Additional ultrastructural observations demonstrated multifocal endothelial injury, platelet recruitment, and alterated hepatocytes. Dengue virus antigen was detected in hepatocytes and in the capillar endothelium of the central lobular vein area. Liver function tests showed high levels of aspartate transaminase and alanine transaminase enzyme activity. Lung tissue showed interstitial pneumonia and mononuclear cells, interseptal oedema, hyperplasia, and hypertrophy of the bronchiolar epithelial cells. DENV-2 led to a transient inflammatory process, but caused focal alterations of the blood-exchange barrier. Viremia was observed from 2nd to 11th day p.i. by isolation of DENV-2 in C6/36 mosquito cell line inoculated with the supernatant of macerated liver, lung, kidney, and cerebellum tissues of the infected mice. |
format |
Article in Journal/Newspaper |
author |
Ortrud Monika Barth Débora Ferreira Barreto Marciano Viana Paes Christina Maeda Takiya Angela Teixeira Pinhão Hermann Gonçalves Schatzmayr |
author_facet |
Ortrud Monika Barth Débora Ferreira Barreto Marciano Viana Paes Christina Maeda Takiya Angela Teixeira Pinhão Hermann Gonçalves Schatzmayr |
author_sort |
Ortrud Monika Barth |
title |
Morphological studies in a model for dengue-2 virus infection in mice |
title_short |
Morphological studies in a model for dengue-2 virus infection in mice |
title_full |
Morphological studies in a model for dengue-2 virus infection in mice |
title_fullStr |
Morphological studies in a model for dengue-2 virus infection in mice |
title_full_unstemmed |
Morphological studies in a model for dengue-2 virus infection in mice |
title_sort |
morphological studies in a model for dengue-2 virus infection in mice |
publisher |
Instituto Oswaldo Cruz, Ministério da Saúde |
publishDate |
2006 |
url |
https://doi.org/10.1590/S0074-02762006000800014 https://doaj.org/article/1aabe40cc2cf4748bdaf33645e0b872f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Memórias do Instituto Oswaldo Cruz., Vol 101, Iss 8, Pp 905-915 (2006) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762006000800014 https://doaj.org/toc/0074-0276 https://doaj.org/toc/1678-8060 doi:10.1590/S0074-02762006000800014 0074-0276 1678-8060 https://doaj.org/article/1aabe40cc2cf4748bdaf33645e0b872f |
op_doi |
https://doi.org/10.1590/S0074-02762006000800014 |
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Memórias do Instituto Oswaldo Cruz |
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