Resistance to systemic inflammation and multi organ damage after global ischemia/reperfusion in the arctic ground squirrel.
Cardiac arrest (CA) and hemorrhagic shock (HS) are two clinically relevant situations where the body undergoes global ischemia as blood pressure drops below the threshold necessary for adequate organ perfusion. Resistance to ischemia/reperfusion (I/R) injury is a characteristic of hibernating mammal...
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ftdoajarticles:oai:doaj.org/article:1a9eedab246641d3af7518eaec6f02f8 2023-05-15T14:31:30+02:00 Resistance to systemic inflammation and multi organ damage after global ischemia/reperfusion in the arctic ground squirrel. Lori K Bogren Jasmine M Olson Joanna Carpluk Jeanette M Moore Kelly L Drew 2014-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0094225 https://doaj.org/article/1a9eedab246641d3af7518eaec6f02f8 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3984146?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0094225 https://doaj.org/article/1a9eedab246641d3af7518eaec6f02f8 PLoS ONE, Vol 9, Iss 4, p e94225 (2014) Medicine R Science Q article 2014 ftdoajarticles https://doi.org/10.1371/journal.pone.0094225 2022-12-31T13:54:47Z Cardiac arrest (CA) and hemorrhagic shock (HS) are two clinically relevant situations where the body undergoes global ischemia as blood pressure drops below the threshold necessary for adequate organ perfusion. Resistance to ischemia/reperfusion (I/R) injury is a characteristic of hibernating mammals. The present study sought to determine if arctic ground squirrels (AGS) are protected from systemic inflammation and multi organ damage after CA- or HS-induced global I/R and if, for HS, this protection is dependent upon their hibernation season.For CA, rats and summer euthermic AGS (AGS-EU) were asphyxiated for 8 min, inducing CA. For HS, rats, AGS-EU, and winter interbout arousal AGS (AGS-IBA) were subject to HS by withdrawing blood to a mean arterial pressure of 35 mmHg and maintaining that pressure for 20 min before reperfusion with Ringers. For both I/R models, body temperature (Tb) was kept at 36.5-37.5°C. After reperfusion, animals were monitored for seven days (CA) or 3 hrs (HS) then tissues and blood were collected for histopathology, clinical chemistries, and cytokine level analysis (HS only). For the HS studies, additional groups of rats and AGS were monitored for three days after HS to access survival and physiological impairment.Rats had increased serum markers of liver damage one hour after CA while AGS did not. For HS, AGS survived 72 hours after I/R whereas rats did not survive overnight. Additionally, only rats displayed an inflammatory response after HS. AGS maintained a positive base excess, whereas the base excess in rats was negative during and after hemorrhage.Regardless of season, AGS are resistant to organ damage, systemic inflammation, and multi organ damage after systemic I/R and this resistance is not dependent on their ability to become decrease Tb during insult but may stem from an altered acid/base and metabolic response during I/R. Article in Journal/Newspaper Arctic ground squirrel Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS ONE 9 4 e94225 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Lori K Bogren Jasmine M Olson Joanna Carpluk Jeanette M Moore Kelly L Drew Resistance to systemic inflammation and multi organ damage after global ischemia/reperfusion in the arctic ground squirrel. |
topic_facet |
Medicine R Science Q |
description |
Cardiac arrest (CA) and hemorrhagic shock (HS) are two clinically relevant situations where the body undergoes global ischemia as blood pressure drops below the threshold necessary for adequate organ perfusion. Resistance to ischemia/reperfusion (I/R) injury is a characteristic of hibernating mammals. The present study sought to determine if arctic ground squirrels (AGS) are protected from systemic inflammation and multi organ damage after CA- or HS-induced global I/R and if, for HS, this protection is dependent upon their hibernation season.For CA, rats and summer euthermic AGS (AGS-EU) were asphyxiated for 8 min, inducing CA. For HS, rats, AGS-EU, and winter interbout arousal AGS (AGS-IBA) were subject to HS by withdrawing blood to a mean arterial pressure of 35 mmHg and maintaining that pressure for 20 min before reperfusion with Ringers. For both I/R models, body temperature (Tb) was kept at 36.5-37.5°C. After reperfusion, animals were monitored for seven days (CA) or 3 hrs (HS) then tissues and blood were collected for histopathology, clinical chemistries, and cytokine level analysis (HS only). For the HS studies, additional groups of rats and AGS were monitored for three days after HS to access survival and physiological impairment.Rats had increased serum markers of liver damage one hour after CA while AGS did not. For HS, AGS survived 72 hours after I/R whereas rats did not survive overnight. Additionally, only rats displayed an inflammatory response after HS. AGS maintained a positive base excess, whereas the base excess in rats was negative during and after hemorrhage.Regardless of season, AGS are resistant to organ damage, systemic inflammation, and multi organ damage after systemic I/R and this resistance is not dependent on their ability to become decrease Tb during insult but may stem from an altered acid/base and metabolic response during I/R. |
format |
Article in Journal/Newspaper |
author |
Lori K Bogren Jasmine M Olson Joanna Carpluk Jeanette M Moore Kelly L Drew |
author_facet |
Lori K Bogren Jasmine M Olson Joanna Carpluk Jeanette M Moore Kelly L Drew |
author_sort |
Lori K Bogren |
title |
Resistance to systemic inflammation and multi organ damage after global ischemia/reperfusion in the arctic ground squirrel. |
title_short |
Resistance to systemic inflammation and multi organ damage after global ischemia/reperfusion in the arctic ground squirrel. |
title_full |
Resistance to systemic inflammation and multi organ damage after global ischemia/reperfusion in the arctic ground squirrel. |
title_fullStr |
Resistance to systemic inflammation and multi organ damage after global ischemia/reperfusion in the arctic ground squirrel. |
title_full_unstemmed |
Resistance to systemic inflammation and multi organ damage after global ischemia/reperfusion in the arctic ground squirrel. |
title_sort |
resistance to systemic inflammation and multi organ damage after global ischemia/reperfusion in the arctic ground squirrel. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pone.0094225 https://doaj.org/article/1a9eedab246641d3af7518eaec6f02f8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic ground squirrel Arctic |
genre_facet |
Arctic ground squirrel Arctic |
op_source |
PLoS ONE, Vol 9, Iss 4, p e94225 (2014) |
op_relation |
http://europepmc.org/articles/PMC3984146?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0094225 https://doaj.org/article/1a9eedab246641d3af7518eaec6f02f8 |
op_doi |
https://doi.org/10.1371/journal.pone.0094225 |
container_title |
PLoS ONE |
container_volume |
9 |
container_issue |
4 |
container_start_page |
e94225 |
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