Assessment of PCR in the detection of Leishmania spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae)
DNA amplification by the polymerase chain reaction (PCR) was applied in the investigation of the presence of Leishmania (Kinetoplastida: Trypanosomatidae) parasites in single phlebotomine sandflies. Three phlebotomine/parasite pairs were used: Lutzomyia longipalpis/Leishmania chagasi, Lutzomyia migo...
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Universidade de São Paulo (USP)
2002
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ftdoajarticles:oai:doaj.org/article:1a6a5e90e07243e594bcac869201a170 2024-09-09T19:25:14+00:00 Assessment of PCR in the detection of Leishmania spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae) MICHALSKY Érika M. FORTES-DIAS Consuelo L. PIMENTA Paulo F.P. SECUNDINO Nágila F.C. DIAS Edelberto S. 2002-01-01T00:00:00Z https://doaj.org/article/1a6a5e90e07243e594bcac869201a170 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652002000500004 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/1a6a5e90e07243e594bcac869201a170 Revista do Instituto de Medicina Tropical de São Paulo, Vol 44, Iss 5, Pp 255-259 (2002) Lutzomyia Leishmania PCR Leishmaniasis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2002 ftdoajarticles 2024-08-05T17:49:30Z DNA amplification by the polymerase chain reaction (PCR) was applied in the investigation of the presence of Leishmania (Kinetoplastida: Trypanosomatidae) parasites in single phlebotomine sandflies. Three phlebotomine/parasite pairs were used: Lutzomyia longipalpis/Leishmania chagasi, Lutzomyia migonei/Leishmania amazonensis and Lutzomyia migonei/Leishmania braziliensis, all of them incriminated in the transmission of visceral or cutaneous leishmaniasis. DNA extraction was performed with whole insects, with no need of previous digestive tract dissection or pooling specimens. The presence of either mouse blood in the digestive tract of the sandflies or the digestive tract itself did not interfere in the PCR. Infection by as few as 10 Leishmania sp. per individual were sufficient for DNA amplification with genus-specific primers. Using primers for L. braziliensis and L. mexicana complexes, respectively, it was possible to discriminate between L. braziliensis and L. amazonensis in experimentally infected vectors (L. migonei). Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Lutzomyia Leishmania PCR Leishmaniasis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Lutzomyia Leishmania PCR Leishmaniasis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 MICHALSKY Érika M. FORTES-DIAS Consuelo L. PIMENTA Paulo F.P. SECUNDINO Nágila F.C. DIAS Edelberto S. Assessment of PCR in the detection of Leishmania spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae) |
topic_facet |
Lutzomyia Leishmania PCR Leishmaniasis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
DNA amplification by the polymerase chain reaction (PCR) was applied in the investigation of the presence of Leishmania (Kinetoplastida: Trypanosomatidae) parasites in single phlebotomine sandflies. Three phlebotomine/parasite pairs were used: Lutzomyia longipalpis/Leishmania chagasi, Lutzomyia migonei/Leishmania amazonensis and Lutzomyia migonei/Leishmania braziliensis, all of them incriminated in the transmission of visceral or cutaneous leishmaniasis. DNA extraction was performed with whole insects, with no need of previous digestive tract dissection or pooling specimens. The presence of either mouse blood in the digestive tract of the sandflies or the digestive tract itself did not interfere in the PCR. Infection by as few as 10 Leishmania sp. per individual were sufficient for DNA amplification with genus-specific primers. Using primers for L. braziliensis and L. mexicana complexes, respectively, it was possible to discriminate between L. braziliensis and L. amazonensis in experimentally infected vectors (L. migonei). |
format |
Article in Journal/Newspaper |
author |
MICHALSKY Érika M. FORTES-DIAS Consuelo L. PIMENTA Paulo F.P. SECUNDINO Nágila F.C. DIAS Edelberto S. |
author_facet |
MICHALSKY Érika M. FORTES-DIAS Consuelo L. PIMENTA Paulo F.P. SECUNDINO Nágila F.C. DIAS Edelberto S. |
author_sort |
MICHALSKY Érika M. |
title |
Assessment of PCR in the detection of Leishmania spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae) |
title_short |
Assessment of PCR in the detection of Leishmania spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae) |
title_full |
Assessment of PCR in the detection of Leishmania spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae) |
title_fullStr |
Assessment of PCR in the detection of Leishmania spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae) |
title_full_unstemmed |
Assessment of PCR in the detection of Leishmania spp in experimentally infected individual phlebotomine sandflies (Diptera: Psychodidae: Phlebotominae) |
title_sort |
assessment of pcr in the detection of leishmania spp in experimentally infected individual phlebotomine sandflies (diptera: psychodidae: phlebotominae) |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2002 |
url |
https://doaj.org/article/1a6a5e90e07243e594bcac869201a170 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 44, Iss 5, Pp 255-259 (2002) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652002000500004 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/1a6a5e90e07243e594bcac869201a170 |
_version_ |
1809894997409923072 |