Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro
Methylmercury (MeHg) is a ubiquitous environmental contaminant which bioaccumulates in marine biota. Fish constitute an important part of a balanced human diet contributing with health beneficial nutrients but may also contain contaminants such as MeHg. Interactions between the marine n-3 fatty acid...
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ftdoajarticles:oai:doaj.org/article:1a53c8bb6774496ab83b49f816d02e5e 2023-05-15T15:32:39+02:00 Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro O. J. Nøstbakken I. L. Bredal P. A. Olsvik T. S. Huang B. E. Torstensen 2012-01-01T00:00:00Z https://doi.org/10.1155/2012/417652 https://doaj.org/article/1a53c8bb6774496ab83b49f816d02e5e EN eng Hindawi Limited http://dx.doi.org/10.1155/2012/417652 https://doaj.org/toc/1110-7243 https://doaj.org/toc/1110-7251 1110-7243 1110-7251 doi:10.1155/2012/417652 https://doaj.org/article/1a53c8bb6774496ab83b49f816d02e5e Journal of Biomedicine and Biotechnology, Vol 2012 (2012) Biotechnology TP248.13-248.65 Medicine R article 2012 ftdoajarticles https://doi.org/10.1155/2012/417652 2022-12-31T13:54:19Z Methylmercury (MeHg) is a ubiquitous environmental contaminant which bioaccumulates in marine biota. Fish constitute an important part of a balanced human diet contributing with health beneficial nutrients but may also contain contaminants such as MeHg. Interactions between the marine n-3 fatty acids eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA) with MeHg-induced toxicity were investigated. Different toxic and metabolic responses were studied in Atlantic salmon kidney (ASK) cell line and the mammalian kidney-derived HEK293 cell line. Both cell lines were preincubated with DHA or EPA prior to MeHg-exposure, and cell toxicity was assessed differently in the cell lines by MeHg-uptake in cells (ASK and HEK293), proliferation (HEK293 and ASK), apoptosis (ASK), oxidation of the red-ox probe roGFP (HEK293), and regulation of selected toxicological and metabolic transcriptional markers (ASK). DHA was observed to decrease the uptake of MeHg in HEK293, but not in ASK cells. DHA also increased, while EPA decreased, MeHg-induced apoptosis in ASK. MeHg exposure induced changes in selected metabolic and known MeHg biomarkers in ASK cells. Both DHA and MeHg, but not EPA, oxidized roGFP in HEK293 cells. In conclusion, marine n-3 fatty acids may ameliorate MeHg toxicity, either by decreasing apoptosis (EPA) or by reducing MeHg uptake (DHA). However, DHA can also augment MeHg toxicity by increasing oxidative stress and apoptosis when combined with MeHg. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles Journal of Biomedicine and Biotechnology 2012 1 13 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Biotechnology TP248.13-248.65 Medicine R |
spellingShingle |
Biotechnology TP248.13-248.65 Medicine R O. J. Nøstbakken I. L. Bredal P. A. Olsvik T. S. Huang B. E. Torstensen Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro |
topic_facet |
Biotechnology TP248.13-248.65 Medicine R |
description |
Methylmercury (MeHg) is a ubiquitous environmental contaminant which bioaccumulates in marine biota. Fish constitute an important part of a balanced human diet contributing with health beneficial nutrients but may also contain contaminants such as MeHg. Interactions between the marine n-3 fatty acids eicosapentaenoic acid (20:5n-3, EPA) and docosahexaenoic acid (22:6n-3, DHA) with MeHg-induced toxicity were investigated. Different toxic and metabolic responses were studied in Atlantic salmon kidney (ASK) cell line and the mammalian kidney-derived HEK293 cell line. Both cell lines were preincubated with DHA or EPA prior to MeHg-exposure, and cell toxicity was assessed differently in the cell lines by MeHg-uptake in cells (ASK and HEK293), proliferation (HEK293 and ASK), apoptosis (ASK), oxidation of the red-ox probe roGFP (HEK293), and regulation of selected toxicological and metabolic transcriptional markers (ASK). DHA was observed to decrease the uptake of MeHg in HEK293, but not in ASK cells. DHA also increased, while EPA decreased, MeHg-induced apoptosis in ASK. MeHg exposure induced changes in selected metabolic and known MeHg biomarkers in ASK cells. Both DHA and MeHg, but not EPA, oxidized roGFP in HEK293 cells. In conclusion, marine n-3 fatty acids may ameliorate MeHg toxicity, either by decreasing apoptosis (EPA) or by reducing MeHg uptake (DHA). However, DHA can also augment MeHg toxicity by increasing oxidative stress and apoptosis when combined with MeHg. |
format |
Article in Journal/Newspaper |
author |
O. J. Nøstbakken I. L. Bredal P. A. Olsvik T. S. Huang B. E. Torstensen |
author_facet |
O. J. Nøstbakken I. L. Bredal P. A. Olsvik T. S. Huang B. E. Torstensen |
author_sort |
O. J. Nøstbakken |
title |
Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro |
title_short |
Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro |
title_full |
Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro |
title_fullStr |
Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro |
title_full_unstemmed |
Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro |
title_sort |
effect of marine omega 3 fatty acids on methylmercury-induced toxicity in fish and mammalian cells in vitro |
publisher |
Hindawi Limited |
publishDate |
2012 |
url |
https://doi.org/10.1155/2012/417652 https://doaj.org/article/1a53c8bb6774496ab83b49f816d02e5e |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Biomedicine and Biotechnology, Vol 2012 (2012) |
op_relation |
http://dx.doi.org/10.1155/2012/417652 https://doaj.org/toc/1110-7243 https://doaj.org/toc/1110-7251 1110-7243 1110-7251 doi:10.1155/2012/417652 https://doaj.org/article/1a53c8bb6774496ab83b49f816d02e5e |
op_doi |
https://doi.org/10.1155/2012/417652 |
container_title |
Journal of Biomedicine and Biotechnology |
container_volume |
2012 |
container_start_page |
1 |
op_container_end_page |
13 |
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1766363137536163840 |