Anti-obesity effect and UHPLC-QTOF-MS/MS based metabolite profiling of Solanum nigrum leaf extract
Objective: To evaluate the antioxidant potential and pancreatic lipase inhibitory action of optimized hydroethanolic extracts of Solanum nigrum. Methods: Optimized extraction for maximum recovery of metabolites was performed using a combination of freeze-drying and ultrasonication followed by determ...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2022
|
Subjects: | |
Online Access: | https://doi.org/10.4103/2221-1691.340561 https://doaj.org/article/19ebd25d99174328b060807a701f56a5 |
id |
ftdoajarticles:oai:doaj.org/article:19ebd25d99174328b060807a701f56a5 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:19ebd25d99174328b060807a701f56a5 2023-05-15T15:10:17+02:00 Anti-obesity effect and UHPLC-QTOF-MS/MS based metabolite profiling of Solanum nigrum leaf extract Zain Ul Aabideen Muhammad Waseem Mumtaz Muhammad Tayyab Akhtar Muhammad Asam Raza Hamid Mukhtar Ahmad Irfan Syed Ali Raza Muhammad Nadeem Yee Soon Ling 2022-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.340561 https://doaj.org/article/19ebd25d99174328b060807a701f56a5 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=4;spage=164;epage=174;aulast=Aabideen https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.340561 https://doaj.org/article/19ebd25d99174328b060807a701f56a5 Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 4, Pp 164-174 (2022) solanum nigrum ultrasonication metabolite profiling total phenolic contents total flavonoid content antioxidant dpph total antioxidant power pancreatic lipase uhplc-qtof-ms/ms antiobesity mice hypolipidemic molecular docking Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.4103/2221-1691.340561 2022-12-31T03:28:27Z Objective: To evaluate the antioxidant potential and pancreatic lipase inhibitory action of optimized hydroethanolic extracts of Solanum nigrum. Methods: Optimized extraction for maximum recovery of metabolites was performed using a combination of freeze-drying and ultrasonication followed by determination of antioxidant and antiobesity properties. The ultra-high performance liquid chromatography equipped with mass spectrometry was used to analyze metabolite profiling of Solanum nigrum. Computational studies were performed using molecular docking and electrostatic potential analysis for individual compounds. The hypolipidemic potential of the most potent extract was assessed in the obese mice fed on fat rich diet. Results: The 80% hydroethanolic extract exhibited the highest extract yield, total phenolic contents, total flavonoid contents along with the strongest 2,2-diphenyl-1-picrylhydrazyl scavenging activity, total antioxidant power, and pancreatic lipase inhibitory properties. The 80% hydroethanolic extract not only regulated the lipid profile of obese mice but also restricted the weight gain in the liver, kidney, and heart. The 80% hydroethanolic extract also reduced alanine transaminase and aspartate transaminase concentrations in serum. The effects of plant extract at 300 mg/kg body weight were quite comparable with the standard drug orlistat. Conclusions: Solanum nigrum is proved as an excellent and potent source of secondary metabolites that might be responsible for obesity mitigation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 12 4 164 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
solanum nigrum ultrasonication metabolite profiling total phenolic contents total flavonoid content antioxidant dpph total antioxidant power pancreatic lipase uhplc-qtof-ms/ms antiobesity mice hypolipidemic molecular docking Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
solanum nigrum ultrasonication metabolite profiling total phenolic contents total flavonoid content antioxidant dpph total antioxidant power pancreatic lipase uhplc-qtof-ms/ms antiobesity mice hypolipidemic molecular docking Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Zain Ul Aabideen Muhammad Waseem Mumtaz Muhammad Tayyab Akhtar Muhammad Asam Raza Hamid Mukhtar Ahmad Irfan Syed Ali Raza Muhammad Nadeem Yee Soon Ling Anti-obesity effect and UHPLC-QTOF-MS/MS based metabolite profiling of Solanum nigrum leaf extract |
topic_facet |
solanum nigrum ultrasonication metabolite profiling total phenolic contents total flavonoid content antioxidant dpph total antioxidant power pancreatic lipase uhplc-qtof-ms/ms antiobesity mice hypolipidemic molecular docking Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the antioxidant potential and pancreatic lipase inhibitory action of optimized hydroethanolic extracts of Solanum nigrum. Methods: Optimized extraction for maximum recovery of metabolites was performed using a combination of freeze-drying and ultrasonication followed by determination of antioxidant and antiobesity properties. The ultra-high performance liquid chromatography equipped with mass spectrometry was used to analyze metabolite profiling of Solanum nigrum. Computational studies were performed using molecular docking and electrostatic potential analysis for individual compounds. The hypolipidemic potential of the most potent extract was assessed in the obese mice fed on fat rich diet. Results: The 80% hydroethanolic extract exhibited the highest extract yield, total phenolic contents, total flavonoid contents along with the strongest 2,2-diphenyl-1-picrylhydrazyl scavenging activity, total antioxidant power, and pancreatic lipase inhibitory properties. The 80% hydroethanolic extract not only regulated the lipid profile of obese mice but also restricted the weight gain in the liver, kidney, and heart. The 80% hydroethanolic extract also reduced alanine transaminase and aspartate transaminase concentrations in serum. The effects of plant extract at 300 mg/kg body weight were quite comparable with the standard drug orlistat. Conclusions: Solanum nigrum is proved as an excellent and potent source of secondary metabolites that might be responsible for obesity mitigation. |
format |
Article in Journal/Newspaper |
author |
Zain Ul Aabideen Muhammad Waseem Mumtaz Muhammad Tayyab Akhtar Muhammad Asam Raza Hamid Mukhtar Ahmad Irfan Syed Ali Raza Muhammad Nadeem Yee Soon Ling |
author_facet |
Zain Ul Aabideen Muhammad Waseem Mumtaz Muhammad Tayyab Akhtar Muhammad Asam Raza Hamid Mukhtar Ahmad Irfan Syed Ali Raza Muhammad Nadeem Yee Soon Ling |
author_sort |
Zain Ul Aabideen |
title |
Anti-obesity effect and UHPLC-QTOF-MS/MS based metabolite profiling of Solanum nigrum leaf extract |
title_short |
Anti-obesity effect and UHPLC-QTOF-MS/MS based metabolite profiling of Solanum nigrum leaf extract |
title_full |
Anti-obesity effect and UHPLC-QTOF-MS/MS based metabolite profiling of Solanum nigrum leaf extract |
title_fullStr |
Anti-obesity effect and UHPLC-QTOF-MS/MS based metabolite profiling of Solanum nigrum leaf extract |
title_full_unstemmed |
Anti-obesity effect and UHPLC-QTOF-MS/MS based metabolite profiling of Solanum nigrum leaf extract |
title_sort |
anti-obesity effect and uhplc-qtof-ms/ms based metabolite profiling of solanum nigrum leaf extract |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2022 |
url |
https://doi.org/10.4103/2221-1691.340561 https://doaj.org/article/19ebd25d99174328b060807a701f56a5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 12, Iss 4, Pp 164-174 (2022) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2022;volume=12;issue=4;spage=164;epage=174;aulast=Aabideen https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.340561 https://doaj.org/article/19ebd25d99174328b060807a701f56a5 |
op_doi |
https://doi.org/10.4103/2221-1691.340561 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
12 |
container_issue |
4 |
container_start_page |
164 |
_version_ |
1766341327890415616 |