Changes in total and differential leukocyte counts during the clinically silent liver phase in a controlled human malaria infection in malaria-naïve Dutch volunteers
Abstract Background Both in endemic countries and in imported malaria, changes in total and differential leukocyte count during Plasmodium falciparum infection have been described. To study the exact dynamics of differential leukocyte counts and their ratios, they were monitored in a group of health...
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ftdoajarticles:oai:doaj.org/article:19e8edf6d68942dabecdb36cd4a1044a 2023-05-15T15:17:45+02:00 Changes in total and differential leukocyte counts during the clinically silent liver phase in a controlled human malaria infection in malaria-naïve Dutch volunteers Marlies E. van Wolfswinkel Marijke C. C. Langenberg Linda J. Wammes Robert W. Sauerwein Rob Koelewijn Cornelus C. Hermsen Jaap J. van Hellemond Perry J. van Genderen 2017-11-01T00:00:00Z https://doi.org/10.1186/s12936-017-2108-1 https://doaj.org/article/19e8edf6d68942dabecdb36cd4a1044a EN eng BMC http://link.springer.com/article/10.1186/s12936-017-2108-1 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-2108-1 1475-2875 https://doaj.org/article/19e8edf6d68942dabecdb36cd4a1044a Malaria Journal, Vol 16, Iss 1, Pp 1-8 (2017) Controlled Human Malaria Infection Plasmodium falciparum Leukocyte count Lymphocyte count Lymphocytopenia Neutropenia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-2108-1 2022-12-30T21:29:04Z Abstract Background Both in endemic countries and in imported malaria, changes in total and differential leukocyte count during Plasmodium falciparum infection have been described. To study the exact dynamics of differential leukocyte counts and their ratios, they were monitored in a group of healthy non-immune volunteers in two separate Controlled Human Malaria Infection (CHMI) studies. Methods In two CHMI trials, CHMI-a and CHMI-b, 15 and 24 healthy malaria-naïve volunteers, respectively, were exposed to bites of infected mosquitoes, using the P. falciparum research strain NF54 and the novel clones NF135.C10 and NF166.C8. After mosquito bite exposure, twice-daily blood draws were taken to detect parasitaemia and to monitor the total and differential leukocyte counts. All subjects received a course of atovaquone–proguanil when meeting the treatment criteria. Results A total of 39 volunteers participated in the two trials. Thirty-five participants, all 15 participants in CHMI-a and 20 of the 24 volunteers in CHMI-b, developed parasitaemia. During liver stage development of the parasite, the median total leukocyte count increased from 5.5 to 6.1 × 109 leukocytes/L (p = 0.005), the median lymphocyte count from 1.9 to 2.2 (p = 0.001) and the monocyte count from 0.50 to 0.54 (p = 0.038). During the subsequent blood stage infection, significant changes in total and differential leukocyte counts lead to a leukocytopenia (nadir median 3.3 × 109 leukocytes/L, p = 0.0001), lymphocytopenia (nadir median 0.7 × 109 lymphocytes/L, p = 0.0001) and a borderline neutropenia (nadir median 1.5 × 109 neutrophils/L, p = 0.0001). The neutrophil to lymphocyte count ratio (NLCR) reached a maximum of 4.0. Significant correlations were found between parasite load and absolute lymphocyte count (p < 0.001, correlation coefficient − 0.46) and between parasite load and NLCR (p < 0.001, correlation coefficient 0.50). All parameters normalized after parasite clearance. Conclusions During the clinically silent liver phase of malaria, an ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Controlled Human Malaria Infection Plasmodium falciparum Leukocyte count Lymphocyte count Lymphocytopenia Neutropenia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Controlled Human Malaria Infection Plasmodium falciparum Leukocyte count Lymphocyte count Lymphocytopenia Neutropenia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Marlies E. van Wolfswinkel Marijke C. C. Langenberg Linda J. Wammes Robert W. Sauerwein Rob Koelewijn Cornelus C. Hermsen Jaap J. van Hellemond Perry J. van Genderen Changes in total and differential leukocyte counts during the clinically silent liver phase in a controlled human malaria infection in malaria-naïve Dutch volunteers |
topic_facet |
Controlled Human Malaria Infection Plasmodium falciparum Leukocyte count Lymphocyte count Lymphocytopenia Neutropenia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Both in endemic countries and in imported malaria, changes in total and differential leukocyte count during Plasmodium falciparum infection have been described. To study the exact dynamics of differential leukocyte counts and their ratios, they were monitored in a group of healthy non-immune volunteers in two separate Controlled Human Malaria Infection (CHMI) studies. Methods In two CHMI trials, CHMI-a and CHMI-b, 15 and 24 healthy malaria-naïve volunteers, respectively, were exposed to bites of infected mosquitoes, using the P. falciparum research strain NF54 and the novel clones NF135.C10 and NF166.C8. After mosquito bite exposure, twice-daily blood draws were taken to detect parasitaemia and to monitor the total and differential leukocyte counts. All subjects received a course of atovaquone–proguanil when meeting the treatment criteria. Results A total of 39 volunteers participated in the two trials. Thirty-five participants, all 15 participants in CHMI-a and 20 of the 24 volunteers in CHMI-b, developed parasitaemia. During liver stage development of the parasite, the median total leukocyte count increased from 5.5 to 6.1 × 109 leukocytes/L (p = 0.005), the median lymphocyte count from 1.9 to 2.2 (p = 0.001) and the monocyte count from 0.50 to 0.54 (p = 0.038). During the subsequent blood stage infection, significant changes in total and differential leukocyte counts lead to a leukocytopenia (nadir median 3.3 × 109 leukocytes/L, p = 0.0001), lymphocytopenia (nadir median 0.7 × 109 lymphocytes/L, p = 0.0001) and a borderline neutropenia (nadir median 1.5 × 109 neutrophils/L, p = 0.0001). The neutrophil to lymphocyte count ratio (NLCR) reached a maximum of 4.0. Significant correlations were found between parasite load and absolute lymphocyte count (p < 0.001, correlation coefficient − 0.46) and between parasite load and NLCR (p < 0.001, correlation coefficient 0.50). All parameters normalized after parasite clearance. Conclusions During the clinically silent liver phase of malaria, an ... |
format |
Article in Journal/Newspaper |
author |
Marlies E. van Wolfswinkel Marijke C. C. Langenberg Linda J. Wammes Robert W. Sauerwein Rob Koelewijn Cornelus C. Hermsen Jaap J. van Hellemond Perry J. van Genderen |
author_facet |
Marlies E. van Wolfswinkel Marijke C. C. Langenberg Linda J. Wammes Robert W. Sauerwein Rob Koelewijn Cornelus C. Hermsen Jaap J. van Hellemond Perry J. van Genderen |
author_sort |
Marlies E. van Wolfswinkel |
title |
Changes in total and differential leukocyte counts during the clinically silent liver phase in a controlled human malaria infection in malaria-naïve Dutch volunteers |
title_short |
Changes in total and differential leukocyte counts during the clinically silent liver phase in a controlled human malaria infection in malaria-naïve Dutch volunteers |
title_full |
Changes in total and differential leukocyte counts during the clinically silent liver phase in a controlled human malaria infection in malaria-naïve Dutch volunteers |
title_fullStr |
Changes in total and differential leukocyte counts during the clinically silent liver phase in a controlled human malaria infection in malaria-naïve Dutch volunteers |
title_full_unstemmed |
Changes in total and differential leukocyte counts during the clinically silent liver phase in a controlled human malaria infection in malaria-naïve Dutch volunteers |
title_sort |
changes in total and differential leukocyte counts during the clinically silent liver phase in a controlled human malaria infection in malaria-naïve dutch volunteers |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-2108-1 https://doaj.org/article/19e8edf6d68942dabecdb36cd4a1044a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-8 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-2108-1 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-2108-1 1475-2875 https://doaj.org/article/19e8edf6d68942dabecdb36cd4a1044a |
op_doi |
https://doi.org/10.1186/s12936-017-2108-1 |
container_title |
Malaria Journal |
container_volume |
16 |
container_issue |
1 |
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1766347993204654080 |