Key role of mitochondrial mutation Leu107Ser (COX1) in deltamethrin resistance in salmon lice (Lepeophtheirus salmonis)
Abstract The pyrethroid deltamethrin (DTM) is used to treat Atlantic salmon (Salmo salar) against salmon louse (Lepeophtheirus salmonis) infestations. However, DTM resistance has evolved in L. salmonis and is currently common in the North Atlantic. This study aimed to re-assess the association betwe...
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ftdoajarticles:oai:doaj.org/article:19e887d5e8ee4f5392dc214f4dbd0212 2023-05-15T15:32:30+02:00 Key role of mitochondrial mutation Leu107Ser (COX1) in deltamethrin resistance in salmon lice (Lepeophtheirus salmonis) Claudia Tschesche Michaël Bekaert David I. Bassett Sally Boyd James E. Bron Armin Sturm 2022-06-01T00:00:00Z https://doi.org/10.1038/s41598-022-14023-1 https://doaj.org/article/19e887d5e8ee4f5392dc214f4dbd0212 EN eng Nature Portfolio https://doi.org/10.1038/s41598-022-14023-1 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-14023-1 2045-2322 https://doaj.org/article/19e887d5e8ee4f5392dc214f4dbd0212 Scientific Reports, Vol 12, Iss 1, Pp 1-13 (2022) Medicine R Science Q article 2022 ftdoajarticles https://doi.org/10.1038/s41598-022-14023-1 2022-12-31T03:15:55Z Abstract The pyrethroid deltamethrin (DTM) is used to treat Atlantic salmon (Salmo salar) against salmon louse (Lepeophtheirus salmonis) infestations. However, DTM resistance has evolved in L. salmonis and is currently common in the North Atlantic. This study aimed to re-assess the association between DTM resistance and mitochondrial (mtDNA) mutations demonstrated in previous reports. Among 218 L. salmonis collected in Scotland in 2018–2019, 89.4% showed DTM resistance in bioassays, while 93.6% expressed at least one of four mtDNA single nucleotide polymorphisms (SNPs) previously shown to be resistance associated. Genotyping at further 14 SNP loci allowed to define three resistance-associated mtDNA haplotypes, named 2, 3 and 4, occurring in 72.0%, 14.2% and 7.3% of samples, respectively. L. salmonis strains IoA-02 (haplotype 2) and IoA-10 (haplotype 3) both showed high levels (~ 100-fold) of DTM resistance, which was inherited maternally in crossing experiments. MtDNA haplotypes 2 and 3 differed in genotype for 17 of 18 studied SNPs, but shared one mutation that causes an amino acid change (Leu107Ser) in the cytochrome c oxidase subunit 1 (COX1) and was present in all DTM resistant while lacking in all susceptible parasites. We conclude that Leu107Ser (COX1) is a main genetic determinant of DTM resistance in L. salmonis. Article in Journal/Newspaper Atlantic salmon North Atlantic Salmo salar Directory of Open Access Journals: DOAJ Articles Scientific Reports 12 1 |
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Medicine R Science Q Claudia Tschesche Michaël Bekaert David I. Bassett Sally Boyd James E. Bron Armin Sturm Key role of mitochondrial mutation Leu107Ser (COX1) in deltamethrin resistance in salmon lice (Lepeophtheirus salmonis) |
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Medicine R Science Q |
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Abstract The pyrethroid deltamethrin (DTM) is used to treat Atlantic salmon (Salmo salar) against salmon louse (Lepeophtheirus salmonis) infestations. However, DTM resistance has evolved in L. salmonis and is currently common in the North Atlantic. This study aimed to re-assess the association between DTM resistance and mitochondrial (mtDNA) mutations demonstrated in previous reports. Among 218 L. salmonis collected in Scotland in 2018–2019, 89.4% showed DTM resistance in bioassays, while 93.6% expressed at least one of four mtDNA single nucleotide polymorphisms (SNPs) previously shown to be resistance associated. Genotyping at further 14 SNP loci allowed to define three resistance-associated mtDNA haplotypes, named 2, 3 and 4, occurring in 72.0%, 14.2% and 7.3% of samples, respectively. L. salmonis strains IoA-02 (haplotype 2) and IoA-10 (haplotype 3) both showed high levels (~ 100-fold) of DTM resistance, which was inherited maternally in crossing experiments. MtDNA haplotypes 2 and 3 differed in genotype for 17 of 18 studied SNPs, but shared one mutation that causes an amino acid change (Leu107Ser) in the cytochrome c oxidase subunit 1 (COX1) and was present in all DTM resistant while lacking in all susceptible parasites. We conclude that Leu107Ser (COX1) is a main genetic determinant of DTM resistance in L. salmonis. |
format |
Article in Journal/Newspaper |
author |
Claudia Tschesche Michaël Bekaert David I. Bassett Sally Boyd James E. Bron Armin Sturm |
author_facet |
Claudia Tschesche Michaël Bekaert David I. Bassett Sally Boyd James E. Bron Armin Sturm |
author_sort |
Claudia Tschesche |
title |
Key role of mitochondrial mutation Leu107Ser (COX1) in deltamethrin resistance in salmon lice (Lepeophtheirus salmonis) |
title_short |
Key role of mitochondrial mutation Leu107Ser (COX1) in deltamethrin resistance in salmon lice (Lepeophtheirus salmonis) |
title_full |
Key role of mitochondrial mutation Leu107Ser (COX1) in deltamethrin resistance in salmon lice (Lepeophtheirus salmonis) |
title_fullStr |
Key role of mitochondrial mutation Leu107Ser (COX1) in deltamethrin resistance in salmon lice (Lepeophtheirus salmonis) |
title_full_unstemmed |
Key role of mitochondrial mutation Leu107Ser (COX1) in deltamethrin resistance in salmon lice (Lepeophtheirus salmonis) |
title_sort |
key role of mitochondrial mutation leu107ser (cox1) in deltamethrin resistance in salmon lice (lepeophtheirus salmonis) |
publisher |
Nature Portfolio |
publishDate |
2022 |
url |
https://doi.org/10.1038/s41598-022-14023-1 https://doaj.org/article/19e887d5e8ee4f5392dc214f4dbd0212 |
genre |
Atlantic salmon North Atlantic Salmo salar |
genre_facet |
Atlantic salmon North Atlantic Salmo salar |
op_source |
Scientific Reports, Vol 12, Iss 1, Pp 1-13 (2022) |
op_relation |
https://doi.org/10.1038/s41598-022-14023-1 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-14023-1 2045-2322 https://doaj.org/article/19e887d5e8ee4f5392dc214f4dbd0212 |
op_doi |
https://doi.org/10.1038/s41598-022-14023-1 |
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Scientific Reports |
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12 |
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