The characterization of Helicobacter pylori DNA associated with ancient human remains recovered from a Canadian glacier.
Helicobacter pylori is a gram-negative bacterium that colonizes the stomach of nearly half of the world's population. Genotypic characterization of H. pylori strains involves the analysis of virulence-associated genes, such as vacA, which has multiple alleles. Previous phylogenetic analyses hav...
Published in: | PLoS ONE |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2011
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0016864 https://doaj.org/article/19b84f4ee4504194ba5a74a7b21657f9 |
id |
ftdoajarticles:oai:doaj.org/article:19b84f4ee4504194ba5a74a7b21657f9 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:19b84f4ee4504194ba5a74a7b21657f9 2023-05-15T16:16:48+02:00 The characterization of Helicobacter pylori DNA associated with ancient human remains recovered from a Canadian glacier. Treena Swanston Monique Haakensen Harry Deneer Ernest G Walker 2011-02-01T00:00:00Z https://doi.org/10.1371/journal.pone.0016864 https://doaj.org/article/19b84f4ee4504194ba5a74a7b21657f9 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3040200?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0016864 https://doaj.org/article/19b84f4ee4504194ba5a74a7b21657f9 PLoS ONE, Vol 6, Iss 2, p e16864 (2011) Medicine R Science Q article 2011 ftdoajarticles https://doi.org/10.1371/journal.pone.0016864 2022-12-31T10:03:54Z Helicobacter pylori is a gram-negative bacterium that colonizes the stomach of nearly half of the world's population. Genotypic characterization of H. pylori strains involves the analysis of virulence-associated genes, such as vacA, which has multiple alleles. Previous phylogenetic analyses have revealed a connection between modern H. pylori strains and the movement of ancient human populations. In this study, H. pylori DNA was amplified from the stomach tissue of the Kwäday Dän Ts'ìnchi individual. This ancient individual was recovered from the Samuel Glacier in Tatshenshini-Alsek Park, British Columbia, Canada on the traditional territory of the Champagne and Aishihik First Nations and radiocarbon dated to a timeframe of approximately AD 1670 to 1850. This is the first ancient H. pylori strain to be characterized with vacA sequence data. The Tatshenshini H. pylori strain has a potential hybrid vacA m2a/m1d middle (m) region allele and a vacA s2 signal (s) region allele. A vacA s2 allele is more commonly identified with Western strains, and this suggests that European strains were present in northwestern Canada during the ancient individual's time. Phylogenetic analysis indicated that the vacA m1d region of the ancient strain clusters with previously published novel Native American strains that are closely related to Asian strains. This indicates a past connection between the Kwäday Dän Ts'ìnchi individual and the ancestors who arrived in the New World thousands of years ago. Article in Journal/Newspaper First Nations Directory of Open Access Journals: DOAJ Articles Canada British Columbia ENVELOPE(-125.003,-125.003,54.000,54.000) Champagne ENVELOPE(-136.483,-136.483,60.788,60.788) Aishihik ENVELOPE(-137.512,-137.512,61.598,61.598) Tatshenshini-Alsek Park ENVELOPE(-137.454,-137.454,59.599,59.599) Samuel Glacier ENVELOPE(-136.804,-136.804,59.699,59.699) PLoS ONE 6 2 e16864 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Treena Swanston Monique Haakensen Harry Deneer Ernest G Walker The characterization of Helicobacter pylori DNA associated with ancient human remains recovered from a Canadian glacier. |
topic_facet |
Medicine R Science Q |
description |
Helicobacter pylori is a gram-negative bacterium that colonizes the stomach of nearly half of the world's population. Genotypic characterization of H. pylori strains involves the analysis of virulence-associated genes, such as vacA, which has multiple alleles. Previous phylogenetic analyses have revealed a connection between modern H. pylori strains and the movement of ancient human populations. In this study, H. pylori DNA was amplified from the stomach tissue of the Kwäday Dän Ts'ìnchi individual. This ancient individual was recovered from the Samuel Glacier in Tatshenshini-Alsek Park, British Columbia, Canada on the traditional territory of the Champagne and Aishihik First Nations and radiocarbon dated to a timeframe of approximately AD 1670 to 1850. This is the first ancient H. pylori strain to be characterized with vacA sequence data. The Tatshenshini H. pylori strain has a potential hybrid vacA m2a/m1d middle (m) region allele and a vacA s2 signal (s) region allele. A vacA s2 allele is more commonly identified with Western strains, and this suggests that European strains were present in northwestern Canada during the ancient individual's time. Phylogenetic analysis indicated that the vacA m1d region of the ancient strain clusters with previously published novel Native American strains that are closely related to Asian strains. This indicates a past connection between the Kwäday Dän Ts'ìnchi individual and the ancestors who arrived in the New World thousands of years ago. |
format |
Article in Journal/Newspaper |
author |
Treena Swanston Monique Haakensen Harry Deneer Ernest G Walker |
author_facet |
Treena Swanston Monique Haakensen Harry Deneer Ernest G Walker |
author_sort |
Treena Swanston |
title |
The characterization of Helicobacter pylori DNA associated with ancient human remains recovered from a Canadian glacier. |
title_short |
The characterization of Helicobacter pylori DNA associated with ancient human remains recovered from a Canadian glacier. |
title_full |
The characterization of Helicobacter pylori DNA associated with ancient human remains recovered from a Canadian glacier. |
title_fullStr |
The characterization of Helicobacter pylori DNA associated with ancient human remains recovered from a Canadian glacier. |
title_full_unstemmed |
The characterization of Helicobacter pylori DNA associated with ancient human remains recovered from a Canadian glacier. |
title_sort |
characterization of helicobacter pylori dna associated with ancient human remains recovered from a canadian glacier. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pone.0016864 https://doaj.org/article/19b84f4ee4504194ba5a74a7b21657f9 |
long_lat |
ENVELOPE(-125.003,-125.003,54.000,54.000) ENVELOPE(-136.483,-136.483,60.788,60.788) ENVELOPE(-137.512,-137.512,61.598,61.598) ENVELOPE(-137.454,-137.454,59.599,59.599) ENVELOPE(-136.804,-136.804,59.699,59.699) |
geographic |
Canada British Columbia Champagne Aishihik Tatshenshini-Alsek Park Samuel Glacier |
geographic_facet |
Canada British Columbia Champagne Aishihik Tatshenshini-Alsek Park Samuel Glacier |
genre |
First Nations |
genre_facet |
First Nations |
op_source |
PLoS ONE, Vol 6, Iss 2, p e16864 (2011) |
op_relation |
http://europepmc.org/articles/PMC3040200?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0016864 https://doaj.org/article/19b84f4ee4504194ba5a74a7b21657f9 |
op_doi |
https://doi.org/10.1371/journal.pone.0016864 |
container_title |
PLoS ONE |
container_volume |
6 |
container_issue |
2 |
container_start_page |
e16864 |
_version_ |
1766002652153380864 |