The bivalve Thyasira cf. gouldi hosts chemoautotrophic symbiont populations with strain level diversity
Invertebrates from various marine habitats form nutritional symbioses with chemosynthetic bacteria. In chemosynthetic symbioses, both the mode of symbiont transmission and the site of bacterial housing can affect the composition of the symbiont population. Vertically transmitted symbionts, as well a...
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ftdoajarticles:oai:doaj.org/article:199906fc24a740678657b6113045e17f 2024-01-07T09:44:55+01:00 The bivalve Thyasira cf. gouldi hosts chemoautotrophic symbiont populations with strain level diversity Bonita McCuaig France Liboiron Suzanne C. Dufour 2017-07-01T00:00:00Z https://doi.org/10.7717/peerj.3597 https://doaj.org/article/199906fc24a740678657b6113045e17f EN eng PeerJ Inc. https://peerj.com/articles/3597.pdf https://peerj.com/articles/3597/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.3597 2167-8359 https://doaj.org/article/199906fc24a740678657b6113045e17f PeerJ, Vol 5, p e3597 (2017) Thyasiridae 16S rRNA Phylogeny Chemosymbiosis Rubisco Medicine R Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.7717/peerj.3597 2023-12-10T01:50:54Z Invertebrates from various marine habitats form nutritional symbioses with chemosynthetic bacteria. In chemosynthetic symbioses, both the mode of symbiont transmission and the site of bacterial housing can affect the composition of the symbiont population. Vertically transmitted symbionts, as well as those hosted intracellularly, are more likely to form clonal populations within their host. Conversely, symbiont populations that are environmentally acquired and extracellular may be more likely to be heterogeneous/mixed within host individuals, as observed in some mytilid bivalves. The symbionts of thyasirid bivalves are also extracellular, but limited 16S rRNA sequencing data suggest that thyasirid individuals contain uniform symbiont populations. In a recent study, Thyasira cf. gouldi individuals from Bonne Bay, Newfoundland, Canada were found to host one of three 16S rRNA phylotypes of sulfur-oxidizing gammaproteobacteria, suggesting environmental acquisition of symbionts and some degree of site-specificity. Here, we use Sanger sequencing of both 16S RNA and the more variable ribulose-1,5-bisphosphate carboxylase (RuBisCO) PCR products to further examine Thyasira cf. gouldi symbiont diversity at the scale of host individuals, as well as to elucidate any temporal or spatial patterns in symbiont diversity within Bonne Bay, and relationships with host OTU or size. We obtained symbiont 16S rRNA and RuBisCO Form II sequences from 54 and 50 host individuals, respectively, during nine sampling trips to three locations over four years. Analyses uncovered the same three closely related 16S rRNA phylotypes obtained previously, as well as three divergent RuBisCO phylotypes; these were found in various pair combinations within host individuals, suggesting incidents of horizontal gene transfer during symbiont evolution. While we found no temporal patterns in phylotype distribution or relationships with host OTU or size, some spatial effects were noted, with some phylotypes only found within particular sampling sites. The ... Article in Journal/Newspaper Newfoundland Directory of Open Access Journals: DOAJ Articles Canada PeerJ 5 e3597 |
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op_collection_id |
ftdoajarticles |
language |
English |
topic |
Thyasiridae 16S rRNA Phylogeny Chemosymbiosis Rubisco Medicine R Biology (General) QH301-705.5 |
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Thyasiridae 16S rRNA Phylogeny Chemosymbiosis Rubisco Medicine R Biology (General) QH301-705.5 Bonita McCuaig France Liboiron Suzanne C. Dufour The bivalve Thyasira cf. gouldi hosts chemoautotrophic symbiont populations with strain level diversity |
topic_facet |
Thyasiridae 16S rRNA Phylogeny Chemosymbiosis Rubisco Medicine R Biology (General) QH301-705.5 |
description |
Invertebrates from various marine habitats form nutritional symbioses with chemosynthetic bacteria. In chemosynthetic symbioses, both the mode of symbiont transmission and the site of bacterial housing can affect the composition of the symbiont population. Vertically transmitted symbionts, as well as those hosted intracellularly, are more likely to form clonal populations within their host. Conversely, symbiont populations that are environmentally acquired and extracellular may be more likely to be heterogeneous/mixed within host individuals, as observed in some mytilid bivalves. The symbionts of thyasirid bivalves are also extracellular, but limited 16S rRNA sequencing data suggest that thyasirid individuals contain uniform symbiont populations. In a recent study, Thyasira cf. gouldi individuals from Bonne Bay, Newfoundland, Canada were found to host one of three 16S rRNA phylotypes of sulfur-oxidizing gammaproteobacteria, suggesting environmental acquisition of symbionts and some degree of site-specificity. Here, we use Sanger sequencing of both 16S RNA and the more variable ribulose-1,5-bisphosphate carboxylase (RuBisCO) PCR products to further examine Thyasira cf. gouldi symbiont diversity at the scale of host individuals, as well as to elucidate any temporal or spatial patterns in symbiont diversity within Bonne Bay, and relationships with host OTU or size. We obtained symbiont 16S rRNA and RuBisCO Form II sequences from 54 and 50 host individuals, respectively, during nine sampling trips to three locations over four years. Analyses uncovered the same three closely related 16S rRNA phylotypes obtained previously, as well as three divergent RuBisCO phylotypes; these were found in various pair combinations within host individuals, suggesting incidents of horizontal gene transfer during symbiont evolution. While we found no temporal patterns in phylotype distribution or relationships with host OTU or size, some spatial effects were noted, with some phylotypes only found within particular sampling sites. The ... |
format |
Article in Journal/Newspaper |
author |
Bonita McCuaig France Liboiron Suzanne C. Dufour |
author_facet |
Bonita McCuaig France Liboiron Suzanne C. Dufour |
author_sort |
Bonita McCuaig |
title |
The bivalve Thyasira cf. gouldi hosts chemoautotrophic symbiont populations with strain level diversity |
title_short |
The bivalve Thyasira cf. gouldi hosts chemoautotrophic symbiont populations with strain level diversity |
title_full |
The bivalve Thyasira cf. gouldi hosts chemoautotrophic symbiont populations with strain level diversity |
title_fullStr |
The bivalve Thyasira cf. gouldi hosts chemoautotrophic symbiont populations with strain level diversity |
title_full_unstemmed |
The bivalve Thyasira cf. gouldi hosts chemoautotrophic symbiont populations with strain level diversity |
title_sort |
bivalve thyasira cf. gouldi hosts chemoautotrophic symbiont populations with strain level diversity |
publisher |
PeerJ Inc. |
publishDate |
2017 |
url |
https://doi.org/10.7717/peerj.3597 https://doaj.org/article/199906fc24a740678657b6113045e17f |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_source |
PeerJ, Vol 5, p e3597 (2017) |
op_relation |
https://peerj.com/articles/3597.pdf https://peerj.com/articles/3597/ https://doaj.org/toc/2167-8359 doi:10.7717/peerj.3597 2167-8359 https://doaj.org/article/199906fc24a740678657b6113045e17f |
op_doi |
https://doi.org/10.7717/peerj.3597 |
container_title |
PeerJ |
container_volume |
5 |
container_start_page |
e3597 |
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1787426352041820160 |