Metabolomics analyses of serum metabolites perturbations associated with Naja atra bite.
Naja atra bite is one of the most common severe snakebites in emergency departments. Unfortunately, the pathophysiological changes caused by Naja atra bite are unclear due to the lack of good animal models. In this study, an animal model of Naja atra bite in Guangxi Bama miniature pigs was establish...
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ftdoajarticles:oai:doaj.org/article:1978ed51e8b94fd49486c3e50eedd9a2 2023-10-09T21:49:13+02:00 Metabolomics analyses of serum metabolites perturbations associated with Naja atra bite. Dongling He Shaocong Hu Zhi Huang Caifeng Mo Xiaoyang Cheng Pengshu Song Yalan Li Tianlin Song Zhezhe Guan Yi Zhou Xuerong Zhang Ming Liao 2023-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011507 https://doaj.org/article/1978ed51e8b94fd49486c3e50eedd9a2 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011507 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011507 https://doaj.org/article/1978ed51e8b94fd49486c3e50eedd9a2 PLoS Neglected Tropical Diseases, Vol 17, Iss 8, p e0011507 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011507 2023-09-10T00:39:13Z Naja atra bite is one of the most common severe snakebites in emergency departments. Unfortunately, the pathophysiological changes caused by Naja atra bite are unclear due to the lack of good animal models. In this study, an animal model of Naja atra bite in Guangxi Bama miniature pigs was established by intramuscular injection at 2 mg/kg of Naja atra venom, and serum metabolites were systematically analyzed using untargeted metabolomic and targeted metabolomic approaches. Untargeted metabolomic analysis revealed that 5045 chromatographic peaks were obtained in ESI+ and 3871 chromatographic peaks were obtained in ESI-. Screening in ESI+ modes and ESI- modes identified 22 and 36 differential metabolites compared to controls. The presence of 8 core metabolites of glutamine, arginine, proline, leucine, phenylalanine, inosine, thymidine and hippuric acid in the process of Naja atra bite was verified by targeted metabolomics significant difference (P<0.05). At the same time, during the verification process of the serum clinical samples with Naja atra bite, we found that the contents of three metabolites of proline, phenylalanine and inosine in the serum of the patients were significantly different from those of the normal human serum (P<0.05). By conducting functional analysis of core and metabolic pathway analysis, we revealed a potential correlation between changes in key metabolites after the Naja atra bite and the resulting pathophysiological alterations, and our research aims to establish a theoretical foundation for the prompt diagnosis and treatment of Naja atra bite. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 8 e0011507 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Dongling He Shaocong Hu Zhi Huang Caifeng Mo Xiaoyang Cheng Pengshu Song Yalan Li Tianlin Song Zhezhe Guan Yi Zhou Xuerong Zhang Ming Liao Metabolomics analyses of serum metabolites perturbations associated with Naja atra bite. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Naja atra bite is one of the most common severe snakebites in emergency departments. Unfortunately, the pathophysiological changes caused by Naja atra bite are unclear due to the lack of good animal models. In this study, an animal model of Naja atra bite in Guangxi Bama miniature pigs was established by intramuscular injection at 2 mg/kg of Naja atra venom, and serum metabolites were systematically analyzed using untargeted metabolomic and targeted metabolomic approaches. Untargeted metabolomic analysis revealed that 5045 chromatographic peaks were obtained in ESI+ and 3871 chromatographic peaks were obtained in ESI-. Screening in ESI+ modes and ESI- modes identified 22 and 36 differential metabolites compared to controls. The presence of 8 core metabolites of glutamine, arginine, proline, leucine, phenylalanine, inosine, thymidine and hippuric acid in the process of Naja atra bite was verified by targeted metabolomics significant difference (P<0.05). At the same time, during the verification process of the serum clinical samples with Naja atra bite, we found that the contents of three metabolites of proline, phenylalanine and inosine in the serum of the patients were significantly different from those of the normal human serum (P<0.05). By conducting functional analysis of core and metabolic pathway analysis, we revealed a potential correlation between changes in key metabolites after the Naja atra bite and the resulting pathophysiological alterations, and our research aims to establish a theoretical foundation for the prompt diagnosis and treatment of Naja atra bite. |
format |
Article in Journal/Newspaper |
author |
Dongling He Shaocong Hu Zhi Huang Caifeng Mo Xiaoyang Cheng Pengshu Song Yalan Li Tianlin Song Zhezhe Guan Yi Zhou Xuerong Zhang Ming Liao |
author_facet |
Dongling He Shaocong Hu Zhi Huang Caifeng Mo Xiaoyang Cheng Pengshu Song Yalan Li Tianlin Song Zhezhe Guan Yi Zhou Xuerong Zhang Ming Liao |
author_sort |
Dongling He |
title |
Metabolomics analyses of serum metabolites perturbations associated with Naja atra bite. |
title_short |
Metabolomics analyses of serum metabolites perturbations associated with Naja atra bite. |
title_full |
Metabolomics analyses of serum metabolites perturbations associated with Naja atra bite. |
title_fullStr |
Metabolomics analyses of serum metabolites perturbations associated with Naja atra bite. |
title_full_unstemmed |
Metabolomics analyses of serum metabolites perturbations associated with Naja atra bite. |
title_sort |
metabolomics analyses of serum metabolites perturbations associated with naja atra bite. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011507 https://doaj.org/article/1978ed51e8b94fd49486c3e50eedd9a2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 8, p e0011507 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011507 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011507 https://doaj.org/article/1978ed51e8b94fd49486c3e50eedd9a2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0011507 |
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PLOS Neglected Tropical Diseases |
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17 |
container_issue |
8 |
container_start_page |
e0011507 |
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1779312229679628288 |