Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens
Objective: To standardize an ELISA protocol to quantify total immunoglobulin A (IgA) from different biological samples. Methods: Two independent experiments were conducted. In Experiment 1, total IgA levels were quantified from the lachrymal fluid, tracheal swab, and cloacal swab at various time poi...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2017
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Online Access: | https://doi.org/10.1016/j.apjtb.2016.11.021 https://doaj.org/article/1962372de9a04a89b0952d584cb252fb |
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ftdoajarticles:oai:doaj.org/article:1962372de9a04a89b0952d584cb252fb 2023-05-15T15:13:39+02:00 Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens Rubén Merino-Guzmán Juan David Latorre Ramiro Delgado Xochitl Hernandez-Velasco Amanda Desha Wolfenden Kyle Dean Teague Lucas Elzie Graham Brittany Danielle Mahaffey Mikayla Francis Ann Baxter Billy Marshall Hargis Guillermo Tellez 2017-02-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.11.021 https://doaj.org/article/1962372de9a04a89b0952d584cb252fb EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116308000 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.11.021 https://doaj.org/article/1962372de9a04a89b0952d584cb252fb Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 2, Pp 116-120 (2017) Immunoglobulin A ELISA Chicken Lachrymal fluid Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.11.021 2022-12-31T11:23:35Z Objective: To standardize an ELISA protocol to quantify total immunoglobulin A (IgA) from different biological samples. Methods: Two independent experiments were conducted. In Experiment 1, total IgA levels were quantified from the lachrymal fluid, tracheal swab, and cloacal swab at various time points from Days 30 to 89 in white Leghorn chickens. Experiment 2 was conducted to evaluate the effect of 50 or 500 ppb of aflatoxin B1 (AFB1) on total IgA quantified in samples from the lachrymal fluid, tracheal swab, gut content and cloacal swab in broiler chickens at 21 days of age. Results: Lachrymal fluid contained the highest level of IgA; however, the sampling procedure was time consuming and stressful to the bird, and the sample volume depends largely on the size of the chicken. Cloacal swabs also contained a high concentration of IgA; this sampling procedure was faster than lachrymal fluid sampling and was not affected by the age of the bird. Tracheal sampling was more difficult than cloacal sampling; the age of the bird limited the sampling, and the IgA concentration was the lowest detected at all sampling ages. 500 ppb of AFB1 significantly reduced total IgA concentration in the gut content compared with control or 50 ppb of AFB1 treated groups. Interestingly, a significant reduction in total IgA was also observed in those chickens that received 50 ppb of AFB1 in gut rinse when compared with cloacal swabs. Conclusions: The results of this study suggest that cloacal swab is an easy and reliable way to evaluate mucosal IgA concentration in both Leghorn and broiler chickens. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 2 116 120 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Immunoglobulin A ELISA Chicken Lachrymal fluid Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Immunoglobulin A ELISA Chicken Lachrymal fluid Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Rubén Merino-Guzmán Juan David Latorre Ramiro Delgado Xochitl Hernandez-Velasco Amanda Desha Wolfenden Kyle Dean Teague Lucas Elzie Graham Brittany Danielle Mahaffey Mikayla Francis Ann Baxter Billy Marshall Hargis Guillermo Tellez Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens |
topic_facet |
Immunoglobulin A ELISA Chicken Lachrymal fluid Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To standardize an ELISA protocol to quantify total immunoglobulin A (IgA) from different biological samples. Methods: Two independent experiments were conducted. In Experiment 1, total IgA levels were quantified from the lachrymal fluid, tracheal swab, and cloacal swab at various time points from Days 30 to 89 in white Leghorn chickens. Experiment 2 was conducted to evaluate the effect of 50 or 500 ppb of aflatoxin B1 (AFB1) on total IgA quantified in samples from the lachrymal fluid, tracheal swab, gut content and cloacal swab in broiler chickens at 21 days of age. Results: Lachrymal fluid contained the highest level of IgA; however, the sampling procedure was time consuming and stressful to the bird, and the sample volume depends largely on the size of the chicken. Cloacal swabs also contained a high concentration of IgA; this sampling procedure was faster than lachrymal fluid sampling and was not affected by the age of the bird. Tracheal sampling was more difficult than cloacal sampling; the age of the bird limited the sampling, and the IgA concentration was the lowest detected at all sampling ages. 500 ppb of AFB1 significantly reduced total IgA concentration in the gut content compared with control or 50 ppb of AFB1 treated groups. Interestingly, a significant reduction in total IgA was also observed in those chickens that received 50 ppb of AFB1 in gut rinse when compared with cloacal swabs. Conclusions: The results of this study suggest that cloacal swab is an easy and reliable way to evaluate mucosal IgA concentration in both Leghorn and broiler chickens. |
format |
Article in Journal/Newspaper |
author |
Rubén Merino-Guzmán Juan David Latorre Ramiro Delgado Xochitl Hernandez-Velasco Amanda Desha Wolfenden Kyle Dean Teague Lucas Elzie Graham Brittany Danielle Mahaffey Mikayla Francis Ann Baxter Billy Marshall Hargis Guillermo Tellez |
author_facet |
Rubén Merino-Guzmán Juan David Latorre Ramiro Delgado Xochitl Hernandez-Velasco Amanda Desha Wolfenden Kyle Dean Teague Lucas Elzie Graham Brittany Danielle Mahaffey Mikayla Francis Ann Baxter Billy Marshall Hargis Guillermo Tellez |
author_sort |
Rubén Merino-Guzmán |
title |
Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens |
title_short |
Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens |
title_full |
Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens |
title_fullStr |
Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens |
title_full_unstemmed |
Comparison of total immunoglobulin A levels in different samples in Leghorn and broiler chickens |
title_sort |
comparison of total immunoglobulin a levels in different samples in leghorn and broiler chickens |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2016.11.021 https://doaj.org/article/1962372de9a04a89b0952d584cb252fb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 2, Pp 116-120 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116308000 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.11.021 https://doaj.org/article/1962372de9a04a89b0952d584cb252fb |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.11.021 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
2 |
container_start_page |
116 |
op_container_end_page |
120 |
_version_ |
1766344185239044096 |