Triterpenoid of avocado (Persea americana) seed and its cytotoxic activity toward breast MCF-7 and liver HepG2 cancer cells
Objective: To determine the structure of triterpenoid isolated from avocado seeds and the cytotoxic effect on MCF-7 and HepG2 cells. Methods: The powder sample was macerated with ethanol, followed with separation of the extract by column chromatography. The target compound was monitored on thin laye...
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Wolters Kluwer Medknow Publications
2017
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ftdoajarticles:oai:doaj.org/article:195b3af17cbc4f20be6c066bf4a90c84 2023-05-15T15:07:54+02:00 Triterpenoid of avocado (Persea americana) seed and its cytotoxic activity toward breast MCF-7 and liver HepG2 cancer cells Andi Nur Fitriani Abubakar Suminar Setiati Achmadi Irma Herawati Suparto 2017-05-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2017.01.010 https://doaj.org/article/195b3af17cbc4f20be6c066bf4a90c84 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S222116911630627X https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.01.010 https://doaj.org/article/195b3af17cbc4f20be6c066bf4a90c84 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 5, Pp 397-400 (2017) Persea americana Triterpenoid MCF-7 HepG2 Cancer cells Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2017.01.010 2022-12-30T21:45:41Z Objective: To determine the structure of triterpenoid isolated from avocado seeds and the cytotoxic effect on MCF-7 and HepG2 cells. Methods: The powder sample was macerated with ethanol, followed with separation of the extract by column chromatography. The target compound was monitored on thin layer chromatography plate and reagent Lieberman–Buchard. The isolated compound was characterized by spectral analysis, mainly ultraviolet, infrared, and liquid chromatography-mass spectroscopy and their spectroscopic data with those reported in literature were compared. In vitro cytotoxic activity was investigated against Vero, MCF-7, and HepG2 cell lines using MTT assay. Results: A triterpenoid compound was isolated from ethanol extract. The extracts, fraction (F3), and the isolated compound showed a significant cytotoxic activity against all investigated cell lines. MTT assay showed that the triterpenoid isolate inhibited cell proliferation of MCF-7 and HepG2 cell line with the IC50 values of 62 μg/mL and 12 μg/mL, respectively, and was safe to normal cells. Conclusions: The results of the present study reveal that triterpenoid from avocado seeds have the potential for further development as anticancer agents. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 5 397 400 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Persea americana Triterpenoid MCF-7 HepG2 Cancer cells Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Persea americana Triterpenoid MCF-7 HepG2 Cancer cells Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Andi Nur Fitriani Abubakar Suminar Setiati Achmadi Irma Herawati Suparto Triterpenoid of avocado (Persea americana) seed and its cytotoxic activity toward breast MCF-7 and liver HepG2 cancer cells |
topic_facet |
Persea americana Triterpenoid MCF-7 HepG2 Cancer cells Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To determine the structure of triterpenoid isolated from avocado seeds and the cytotoxic effect on MCF-7 and HepG2 cells. Methods: The powder sample was macerated with ethanol, followed with separation of the extract by column chromatography. The target compound was monitored on thin layer chromatography plate and reagent Lieberman–Buchard. The isolated compound was characterized by spectral analysis, mainly ultraviolet, infrared, and liquid chromatography-mass spectroscopy and their spectroscopic data with those reported in literature were compared. In vitro cytotoxic activity was investigated against Vero, MCF-7, and HepG2 cell lines using MTT assay. Results: A triterpenoid compound was isolated from ethanol extract. The extracts, fraction (F3), and the isolated compound showed a significant cytotoxic activity against all investigated cell lines. MTT assay showed that the triterpenoid isolate inhibited cell proliferation of MCF-7 and HepG2 cell line with the IC50 values of 62 μg/mL and 12 μg/mL, respectively, and was safe to normal cells. Conclusions: The results of the present study reveal that triterpenoid from avocado seeds have the potential for further development as anticancer agents. |
format |
Article in Journal/Newspaper |
author |
Andi Nur Fitriani Abubakar Suminar Setiati Achmadi Irma Herawati Suparto |
author_facet |
Andi Nur Fitriani Abubakar Suminar Setiati Achmadi Irma Herawati Suparto |
author_sort |
Andi Nur Fitriani Abubakar |
title |
Triterpenoid of avocado (Persea americana) seed and its cytotoxic activity toward breast MCF-7 and liver HepG2 cancer cells |
title_short |
Triterpenoid of avocado (Persea americana) seed and its cytotoxic activity toward breast MCF-7 and liver HepG2 cancer cells |
title_full |
Triterpenoid of avocado (Persea americana) seed and its cytotoxic activity toward breast MCF-7 and liver HepG2 cancer cells |
title_fullStr |
Triterpenoid of avocado (Persea americana) seed and its cytotoxic activity toward breast MCF-7 and liver HepG2 cancer cells |
title_full_unstemmed |
Triterpenoid of avocado (Persea americana) seed and its cytotoxic activity toward breast MCF-7 and liver HepG2 cancer cells |
title_sort |
triterpenoid of avocado (persea americana) seed and its cytotoxic activity toward breast mcf-7 and liver hepg2 cancer cells |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2017.01.010 https://doaj.org/article/195b3af17cbc4f20be6c066bf4a90c84 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 5, Pp 397-400 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S222116911630627X https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2017.01.010 https://doaj.org/article/195b3af17cbc4f20be6c066bf4a90c84 |
op_doi |
https://doi.org/10.1016/j.apjtb.2017.01.010 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
5 |
container_start_page |
397 |
op_container_end_page |
400 |
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1766339331695312896 |