Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience
Increases in Arctic temperatures have accelerated melting of the Greenland icesheet, exposing intertidal organisms, such as the blue mussel Mytilus edulis, to high air temperatures and low salinities in summer. However, the interaction of these combined stressors is poorly described at the transcrip...
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ftdoajarticles:oai:doaj.org/article:1955ad7366394693ac254261a11c2e84 2023-05-15T14:58:40+02:00 Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience Nicholas J. Barrett Jakob Thyrring Elizabeth M. Harper Mikael K. Sejr Jesper G. Sørensen Lloyd S. Peck Melody S. Clark 2022-01-01T00:00:00Z https://doi.org/10.3390/genes13010155 https://doaj.org/article/1955ad7366394693ac254261a11c2e84 EN eng MDPI AG https://www.mdpi.com/2073-4425/13/1/155 https://doaj.org/toc/2073-4425 doi:10.3390/genes13010155 2073-4425 https://doaj.org/article/1955ad7366394693ac254261a11c2e84 Genes, Vol 13, Iss 155, p 155 (2022) blue mussel cellular stress response salinity thermal tolerance transcriptome acclimation Genetics QH426-470 article 2022 ftdoajarticles https://doi.org/10.3390/genes13010155 2022-12-31T07:39:39Z Increases in Arctic temperatures have accelerated melting of the Greenland icesheet, exposing intertidal organisms, such as the blue mussel Mytilus edulis, to high air temperatures and low salinities in summer. However, the interaction of these combined stressors is poorly described at the transcriptional level. Comparing expression profiles of M. edulis from experimentally warmed (30 °C and 33 °C) animals kept at control (23‰) and low salinities (15‰) revealed a significant lack of enrichment for Gene Ontology terms (GO), indicating that similar processes were active under all conditions. However, there was a progressive increase in the abundance of upregulated genes as each stressor was applied, with synergistic increases at 33 °C and 15‰, suggesting combined stressors push the animal towards their tolerance thresholds. Further analyses comparing the effects of salinity alone (23‰, 15‰ and 5‰) showed high expression of stress and osmoregulatory marker genes at the lowest salinity, implying that the cell is carrying out intracellular osmoregulation to maintain the cytosol as hyperosmotic. Identification of aquaporins and vacuolar-type ATPase transcripts suggested the cell may use fluid-filled cavities to excrete excess intracellular water, as previously identified in embryonic freshwater mussels. These results indicate that M. edulis has considerable resilience to heat stress and highly efficient mechanisms to acclimatise to lowered salinity in a changing world. Article in Journal/Newspaper Arctic Greenland Directory of Open Access Journals: DOAJ Articles Arctic Greenland Genes 13 1 155 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
blue mussel cellular stress response salinity thermal tolerance transcriptome acclimation Genetics QH426-470 |
spellingShingle |
blue mussel cellular stress response salinity thermal tolerance transcriptome acclimation Genetics QH426-470 Nicholas J. Barrett Jakob Thyrring Elizabeth M. Harper Mikael K. Sejr Jesper G. Sørensen Lloyd S. Peck Melody S. Clark Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience |
topic_facet |
blue mussel cellular stress response salinity thermal tolerance transcriptome acclimation Genetics QH426-470 |
description |
Increases in Arctic temperatures have accelerated melting of the Greenland icesheet, exposing intertidal organisms, such as the blue mussel Mytilus edulis, to high air temperatures and low salinities in summer. However, the interaction of these combined stressors is poorly described at the transcriptional level. Comparing expression profiles of M. edulis from experimentally warmed (30 °C and 33 °C) animals kept at control (23‰) and low salinities (15‰) revealed a significant lack of enrichment for Gene Ontology terms (GO), indicating that similar processes were active under all conditions. However, there was a progressive increase in the abundance of upregulated genes as each stressor was applied, with synergistic increases at 33 °C and 15‰, suggesting combined stressors push the animal towards their tolerance thresholds. Further analyses comparing the effects of salinity alone (23‰, 15‰ and 5‰) showed high expression of stress and osmoregulatory marker genes at the lowest salinity, implying that the cell is carrying out intracellular osmoregulation to maintain the cytosol as hyperosmotic. Identification of aquaporins and vacuolar-type ATPase transcripts suggested the cell may use fluid-filled cavities to excrete excess intracellular water, as previously identified in embryonic freshwater mussels. These results indicate that M. edulis has considerable resilience to heat stress and highly efficient mechanisms to acclimatise to lowered salinity in a changing world. |
format |
Article in Journal/Newspaper |
author |
Nicholas J. Barrett Jakob Thyrring Elizabeth M. Harper Mikael K. Sejr Jesper G. Sørensen Lloyd S. Peck Melody S. Clark |
author_facet |
Nicholas J. Barrett Jakob Thyrring Elizabeth M. Harper Mikael K. Sejr Jesper G. Sørensen Lloyd S. Peck Melody S. Clark |
author_sort |
Nicholas J. Barrett |
title |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience |
title_short |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience |
title_full |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience |
title_fullStr |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience |
title_full_unstemmed |
Molecular Responses to Thermal and Osmotic Stress in Arctic Intertidal Mussels ( Mytilus edulis ): The Limits of Resilience |
title_sort |
molecular responses to thermal and osmotic stress in arctic intertidal mussels ( mytilus edulis ): the limits of resilience |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/genes13010155 https://doaj.org/article/1955ad7366394693ac254261a11c2e84 |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Greenland |
genre_facet |
Arctic Greenland |
op_source |
Genes, Vol 13, Iss 155, p 155 (2022) |
op_relation |
https://www.mdpi.com/2073-4425/13/1/155 https://doaj.org/toc/2073-4425 doi:10.3390/genes13010155 2073-4425 https://doaj.org/article/1955ad7366394693ac254261a11c2e84 |
op_doi |
https://doi.org/10.3390/genes13010155 |
container_title |
Genes |
container_volume |
13 |
container_issue |
1 |
container_start_page |
155 |
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1766330794291232768 |