In vitro antihistamine-releasing activity of a peptide derived from wasp venom of Vespa orientalis
Objective: To investigate the antihistamine-releasing effect of a peptide isolated from wasp venom of Vespa orientalis. Methods: This peptide was separated from crude venom by chromatography methods and mass spectrometry. Then various concentrations (2, 4, 8, 16, 32, 64, 128 and 256 μmol/L) of the p...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2016
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Online Access: | https://doi.org/10.1016/j.apjtb.2015.12.001 https://doaj.org/article/192561ed38734f07901b448b7332f36c |
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ftdoajarticles:oai:doaj.org/article:192561ed38734f07901b448b7332f36c 2023-05-15T15:05:17+02:00 In vitro antihistamine-releasing activity of a peptide derived from wasp venom of Vespa orientalis Jafar Jalaei Mehdi Fazeli Hamid Rajaian Somayeh Layeghi Ghalehsoukhteh Alireza Dehghani Dominic Winter 2016-03-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.12.001 https://doaj.org/article/192561ed38734f07901b448b7332f36c EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115303828 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.12.001 https://doaj.org/article/192561ed38734f07901b448b7332f36c Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 3, Pp 259-264 (2016) Vespa orientalis Antihistamine release Lactate dehydrogenase assay Peptide Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.12.001 2022-12-31T13:48:05Z Objective: To investigate the antihistamine-releasing effect of a peptide isolated from wasp venom of Vespa orientalis. Methods: This peptide was separated from crude venom by chromatography methods and mass spectrometry. Then various concentrations (2, 4, 8, 16, 32, 64, 128 and 256 μmol/L) of the peptide were incubated with mast cells and lactate dehydrogenase assay was performed. Results: No significant effect was observed in lactate dehydrogenase absorbance under 128 μmol/L concentration. This implied that the peptide did not cause cell death in mast cells and consequently, histamine release did not happen. Moreover, the results showed the IC50 of mast cells degranulation at 126 μmol/L, which was approximately high implying that this peptide had high selectivity for normal cells and did not cause histamine release from these cells. Conclusions: This would be a great aim in new drug development, in which an agent acts potentially on its target tissue without activating the immune system. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 3 259 264 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Vespa orientalis Antihistamine release Lactate dehydrogenase assay Peptide Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Vespa orientalis Antihistamine release Lactate dehydrogenase assay Peptide Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Jafar Jalaei Mehdi Fazeli Hamid Rajaian Somayeh Layeghi Ghalehsoukhteh Alireza Dehghani Dominic Winter In vitro antihistamine-releasing activity of a peptide derived from wasp venom of Vespa orientalis |
topic_facet |
Vespa orientalis Antihistamine release Lactate dehydrogenase assay Peptide Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the antihistamine-releasing effect of a peptide isolated from wasp venom of Vespa orientalis. Methods: This peptide was separated from crude venom by chromatography methods and mass spectrometry. Then various concentrations (2, 4, 8, 16, 32, 64, 128 and 256 μmol/L) of the peptide were incubated with mast cells and lactate dehydrogenase assay was performed. Results: No significant effect was observed in lactate dehydrogenase absorbance under 128 μmol/L concentration. This implied that the peptide did not cause cell death in mast cells and consequently, histamine release did not happen. Moreover, the results showed the IC50 of mast cells degranulation at 126 μmol/L, which was approximately high implying that this peptide had high selectivity for normal cells and did not cause histamine release from these cells. Conclusions: This would be a great aim in new drug development, in which an agent acts potentially on its target tissue without activating the immune system. |
format |
Article in Journal/Newspaper |
author |
Jafar Jalaei Mehdi Fazeli Hamid Rajaian Somayeh Layeghi Ghalehsoukhteh Alireza Dehghani Dominic Winter |
author_facet |
Jafar Jalaei Mehdi Fazeli Hamid Rajaian Somayeh Layeghi Ghalehsoukhteh Alireza Dehghani Dominic Winter |
author_sort |
Jafar Jalaei |
title |
In vitro antihistamine-releasing activity of a peptide derived from wasp venom of Vespa orientalis |
title_short |
In vitro antihistamine-releasing activity of a peptide derived from wasp venom of Vespa orientalis |
title_full |
In vitro antihistamine-releasing activity of a peptide derived from wasp venom of Vespa orientalis |
title_fullStr |
In vitro antihistamine-releasing activity of a peptide derived from wasp venom of Vespa orientalis |
title_full_unstemmed |
In vitro antihistamine-releasing activity of a peptide derived from wasp venom of Vespa orientalis |
title_sort |
in vitro antihistamine-releasing activity of a peptide derived from wasp venom of vespa orientalis |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2015.12.001 https://doaj.org/article/192561ed38734f07901b448b7332f36c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 3, Pp 259-264 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115303828 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.12.001 https://doaj.org/article/192561ed38734f07901b448b7332f36c |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.12.001 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
3 |
container_start_page |
259 |
op_container_end_page |
264 |
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1766337021841768448 |