Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette.
Parasitic helminths such as schistosomes, as well as filarial and soil-transmitted nematodes, are estimated to infect at least a billion people worldwide, with devastating impacts on human health and economic development. Diagnosis and monitoring of infection dynamics and efficacy of treatment depen...
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ftdoajarticles:oai:doaj.org/article:18b96819f37248ada2a77dcbab01f106 2023-05-15T15:09:51+02:00 Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette. Jinzhao Song Changchun Liu Swarna Bais Michael G Mauk Haim H Bau Robert M Greenberg 2015-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004318 https://doaj.org/article/18b96819f37248ada2a77dcbab01f106 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4700990?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004318 https://doaj.org/article/18b96819f37248ada2a77dcbab01f106 PLoS Neglected Tropical Diseases, Vol 9, Iss 12, p e0004318 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004318 2022-12-31T15:00:28Z Parasitic helminths such as schistosomes, as well as filarial and soil-transmitted nematodes, are estimated to infect at least a billion people worldwide, with devastating impacts on human health and economic development. Diagnosis and monitoring of infection dynamics and efficacy of treatment depend almost entirely on methods that are inaccurate, labor-intensive, and unreliable. These shortcomings are amplified and take on added significance in mass drug administration programs, where measures of effectiveness depend on accurate monitoring of treatment success (or failure), changes in disease transmission rates, and emergence of possible drug resistance. Here, we adapt isothermal molecular assays such as loop-mediated isothermal amplification (LAMP) to a simple, hand-held, custom-made field-ready microfluidic device that allows sensitive and specific detection of schistosome cell-free nucleic acids in serum and plasma (separated with a point-of-care plasma separator) from Schistosoma mansoni-infected mice. Cell-free S. mansoni DNA was detected with our device without prior extraction from blood. Our chip exhibits high sensitivity (~2 x 10(-17) g/μL), with a positive signal for S. mansoni DNA detectable as early as one week post infection, several weeks before parasite egg production commences. These results indicate that incorporation of isothermal amplification strategies with our chips could represent a strategy for rapid, simple, low-cost diagnosis of both pre-patent and chronic schistosome infections as well as potential monitoring of treatment efficacy. Article in Journal/Newspaper Arctic Human health Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 12 e0004318 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jinzhao Song Changchun Liu Swarna Bais Michael G Mauk Haim H Bau Robert M Greenberg Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Parasitic helminths such as schistosomes, as well as filarial and soil-transmitted nematodes, are estimated to infect at least a billion people worldwide, with devastating impacts on human health and economic development. Diagnosis and monitoring of infection dynamics and efficacy of treatment depend almost entirely on methods that are inaccurate, labor-intensive, and unreliable. These shortcomings are amplified and take on added significance in mass drug administration programs, where measures of effectiveness depend on accurate monitoring of treatment success (or failure), changes in disease transmission rates, and emergence of possible drug resistance. Here, we adapt isothermal molecular assays such as loop-mediated isothermal amplification (LAMP) to a simple, hand-held, custom-made field-ready microfluidic device that allows sensitive and specific detection of schistosome cell-free nucleic acids in serum and plasma (separated with a point-of-care plasma separator) from Schistosoma mansoni-infected mice. Cell-free S. mansoni DNA was detected with our device without prior extraction from blood. Our chip exhibits high sensitivity (~2 x 10(-17) g/μL), with a positive signal for S. mansoni DNA detectable as early as one week post infection, several weeks before parasite egg production commences. These results indicate that incorporation of isothermal amplification strategies with our chips could represent a strategy for rapid, simple, low-cost diagnosis of both pre-patent and chronic schistosome infections as well as potential monitoring of treatment efficacy. |
format |
Article in Journal/Newspaper |
author |
Jinzhao Song Changchun Liu Swarna Bais Michael G Mauk Haim H Bau Robert M Greenberg |
author_facet |
Jinzhao Song Changchun Liu Swarna Bais Michael G Mauk Haim H Bau Robert M Greenberg |
author_sort |
Jinzhao Song |
title |
Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette. |
title_short |
Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette. |
title_full |
Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette. |
title_fullStr |
Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette. |
title_full_unstemmed |
Molecular Detection of Schistosome Infections with a Disposable Microfluidic Cassette. |
title_sort |
molecular detection of schistosome infections with a disposable microfluidic cassette. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0004318 https://doaj.org/article/18b96819f37248ada2a77dcbab01f106 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Human health |
genre_facet |
Arctic Human health |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 12, p e0004318 (2015) |
op_relation |
http://europepmc.org/articles/PMC4700990?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004318 https://doaj.org/article/18b96819f37248ada2a77dcbab01f106 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004318 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
12 |
container_start_page |
e0004318 |
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1766340960228212736 |