High glucose induces myocardial cell injury through increasing reactive oxygen species production
Objective: To study the injury effect and molecular mechanism of high glucose on myocardial cells. Methods: Myocardial cells H9c2 were cultured and divided into the control group treated with DMEM containing 5.5 mmol/L glucose, the high glucose group treated with DMEM containing 35 mmol/L glucose, a...
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ftdoajarticles:oai:doaj.org/article:188b08266312409593050fb1cbe1a04d 2023-05-15T15:11:49+02:00 High glucose induces myocardial cell injury through increasing reactive oxygen species production Yu-Jun Wang Xiao-Yu Lyu Li Yu 2018-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.223575 https://doaj.org/article/188b08266312409593050fb1cbe1a04d EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=1;spage=63;epage=67;aulast=Wang https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.223575 https://doaj.org/article/188b08266312409593050fb1cbe1a04d Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 1, Pp 63-67 (2018) diabetic cardiomyopathy high glucose myocardial cells reactive oxygen species apoptosis collagen Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.4103/1995-7645.223575 2022-12-31T11:26:17Z Objective: To study the injury effect and molecular mechanism of high glucose on myocardial cells. Methods: Myocardial cells H9c2 were cultured and divided into the control group treated with DMEM containing 5.5 mmol/L glucose, the high glucose group treated with DMEM containing 35 mmol/L glucose, and the N-acetylcysteine (NAC) group pre-treated with 1 000 μmol/L NAC and treated with DMEM containing 1 000 μmol/L NAC and 35 mmol/L glucose. The production of ROS and the expression of mitochondria pathway apoptosis molecules in cells as well as the contents of collagen and collagen metabolism molecules were measured. Results: After 8 h, 16 h and 24 h of treatment, ROS RFU as well as Bax, CytC, Caspase-3 and Caspase-9 protein expression in cells and Col-I, Col-III, PINP and PIIINP protein levels in culture medium of high glucose group were higher than those of control group, Bcl-2 protein expression were lower than those of control group, but CTX-I protein levels in culture medium were not significantly different from those of control group; after 24 h of treatment, Bax, CytC, Caspase-3 and Caspase-9 protein expression in cells as well as Col-I, Col-III, PINP and PIIINP protein levels in culture medium of NAC group were lower than those of high glucose group whereas Bcl-2 protein expression was higher than that of high glucose group. Conclusions: High glucose can induce myocardial cell apoptosis, increase collagen synthesis and accelerate interstitial fibrosis by increasing the production of reactive oxygen species. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Medicine 11 1 63 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
diabetic cardiomyopathy high glucose myocardial cells reactive oxygen species apoptosis collagen Arctic medicine. Tropical medicine RC955-962 |
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diabetic cardiomyopathy high glucose myocardial cells reactive oxygen species apoptosis collagen Arctic medicine. Tropical medicine RC955-962 Yu-Jun Wang Xiao-Yu Lyu Li Yu High glucose induces myocardial cell injury through increasing reactive oxygen species production |
topic_facet |
diabetic cardiomyopathy high glucose myocardial cells reactive oxygen species apoptosis collagen Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To study the injury effect and molecular mechanism of high glucose on myocardial cells. Methods: Myocardial cells H9c2 were cultured and divided into the control group treated with DMEM containing 5.5 mmol/L glucose, the high glucose group treated with DMEM containing 35 mmol/L glucose, and the N-acetylcysteine (NAC) group pre-treated with 1 000 μmol/L NAC and treated with DMEM containing 1 000 μmol/L NAC and 35 mmol/L glucose. The production of ROS and the expression of mitochondria pathway apoptosis molecules in cells as well as the contents of collagen and collagen metabolism molecules were measured. Results: After 8 h, 16 h and 24 h of treatment, ROS RFU as well as Bax, CytC, Caspase-3 and Caspase-9 protein expression in cells and Col-I, Col-III, PINP and PIIINP protein levels in culture medium of high glucose group were higher than those of control group, Bcl-2 protein expression were lower than those of control group, but CTX-I protein levels in culture medium were not significantly different from those of control group; after 24 h of treatment, Bax, CytC, Caspase-3 and Caspase-9 protein expression in cells as well as Col-I, Col-III, PINP and PIIINP protein levels in culture medium of NAC group were lower than those of high glucose group whereas Bcl-2 protein expression was higher than that of high glucose group. Conclusions: High glucose can induce myocardial cell apoptosis, increase collagen synthesis and accelerate interstitial fibrosis by increasing the production of reactive oxygen species. |
format |
Article in Journal/Newspaper |
author |
Yu-Jun Wang Xiao-Yu Lyu Li Yu |
author_facet |
Yu-Jun Wang Xiao-Yu Lyu Li Yu |
author_sort |
Yu-Jun Wang |
title |
High glucose induces myocardial cell injury through increasing reactive oxygen species production |
title_short |
High glucose induces myocardial cell injury through increasing reactive oxygen species production |
title_full |
High glucose induces myocardial cell injury through increasing reactive oxygen species production |
title_fullStr |
High glucose induces myocardial cell injury through increasing reactive oxygen species production |
title_full_unstemmed |
High glucose induces myocardial cell injury through increasing reactive oxygen species production |
title_sort |
high glucose induces myocardial cell injury through increasing reactive oxygen species production |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/1995-7645.223575 https://doaj.org/article/188b08266312409593050fb1cbe1a04d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 1, Pp 63-67 (2018) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=1;spage=63;epage=67;aulast=Wang https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.223575 https://doaj.org/article/188b08266312409593050fb1cbe1a04d |
op_doi |
https://doi.org/10.4103/1995-7645.223575 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
11 |
container_issue |
1 |
container_start_page |
63 |
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1766342608070639616 |